Overview

  • Product name

    Anti-HNF1 alpha antibody [EPR23054-142]
    See all HNF1 alpha primary antibodies
  • Description

    Rabbit monoclonal [EPR23054-142] to HNF1 alpha
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
    Unsuitable for: ICC/IF or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human HNF1 alpha aa 300 to the C-terminus. The exact sequence is proprietary.
    Database link: P20823

  • Positive control

    • WB: HepG2, HeLa and Caco-2 lysates. IHC-P: Human pancreas and Human liver tissues.

Properties

Applications

Our Abpromise guarantee covers the use of ab242140 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/2000.
WB 1/1000. Predicted molecular weight: 67 kDa.
  • Application notes
    Is unsuitable for ICC/IF or IP.
  • Target

    • Function

      Transcriptional activator that regulates the tissue specific expression of multiple genes, especially in pancreatic islet cells and in liver. Required for the expression of several liver specific genes. Binds to the inverted palindrome 5'-GTTAATNATTAAC-3'.
    • Tissue specificity

      Liver.
    • Involvement in disease

      Defects in HNF1A are a cause of hepatic adenomas familial (HEPAF) [MIM:142330]. Hepatic adenomas are rare benign liver tumors of presumable epithelial origin that develop in an otherwise normal liver. Hepatic adenomas may be single or multiple. They consist of sheets of well-differentiated hepatocytes that contain fat and glycogen and can produce bile. Bile ducts or portal areas are absent. Kupffer cells, if present, are reduced in number and are non-functional. Conditions associated with adenomas are insulin-dependent diabetes mellitus and glycogen storage diseases (types 1 and 3). Note=Bi-allelic inactivation of HNF1A, whether sporadic or associated with MODY3, may be an early step in the developmant of some hepatocellular carcinomas.
      Defects in HNF1A are the cause of maturity-onset diabetes of the young type 3 (MODY3) [MIM:600496]; also symbolized MODY-3. MODY is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease.
      Defects in HNF1A are the cause of susceptibility to diabetes mellitus insulin-dependent type 20 (IDDM20) [MIM:612520]. IDDM20 is a multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical fetaures are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These features can result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels.
    • Sequence similarities

      Belongs to the HNF1 homeobox family.
      Contains 1 homeobox DNA-binding domain.
    • Cellular localization

      Nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • Albumin proximal factor antibody
      • Hepatic nuclear factor 1 alpha antibody
      • Hepatic nuclear factor 1 antibody
      • Hepatic transcription factor 1 alpha antibody
      • Hepatic transcription factor 1 antibody
      • Hepatocyte nuclear factor 1-alpha antibody
      • HNF 1 antibody
      • HNF 1A antibody
      • HNF-1-alpha antibody
      • HNF-1A antibody
      • hnf1a antibody
      • HNF1A_HUMAN antibody
      • Interferon production regulator factor antibody
      • LF B1 antibody
      • LF B1 hepatic nuclear factor antibody
      • LFB 1 antibody
      • LFB1 antibody
      • LFB1 hepatic nuclear factor antibody
      • Liver specific transcription factor LF B1 antibody
      • Liver specific transcription factor LFB1 antibody
      • Liver-specific transcription factor LF-B1 antibody
      • Maturity onset diabetes of the young 3 antibody
      • MODY 3 antibody
      • MODY3 antibody
      • TCF 1 antibody
      • TCF-1 antibody
      • TCF1 antibody
      • Transcription factor 1 antibody
      • Transcription factor 1 hepatic antibody
      see all

    Images

    • Anti-HNF1 alpha antibody [EPR23054-142] (ab242140) at 1/1000 dilution + Caco-2 (human colorectal adenocarcinoma epithelial cell), whole cell lysate at 10 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

      Predicted band size: 67 kDa
      Observed band size: 81 kDa
      why is the actual band size different from the predicted?



      Blocking and diluting buffer and concentration: 5% NFDM/TBST Lysates should be made freshly and used in WB immediately to minimize protein degradation.  The molecular weight observed is consistent with what has been described in the literature (PMID:31145732).  Exposure time: 6 seconds

    • All lanes : Anti-HNF1 alpha antibody [EPR23054-142] (ab242140) at 1/1000 dilution

      Lane 1 : HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate
      Lane 2 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

      Predicted band size: 67 kDa
      Observed band size: 81 kDa why is the actual band size different from the predicted?



      Blocking and diluting buffer and concentration: 5% NFDM/TBST

      Lysates should be made freshly and used in WB immediately to minimize protein degradation.  The molecular weight observed is consistent with what has been described in the literature (PMID:31145732). 

      Negative control: HeLa (PMID: 12488960) 

      Exposure time: 5.5 seconds

    • Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling HNF1A with ab242140 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on human liver (PMID: 26169608) is observed. The section was incubated with ab242140 for 15 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    • Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling HNF1A with ab242140 at 1/2000 followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human pancreas (PMID: 25793983) is observed. The section was incubated with ab242140 for 15 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    References

    ab242140 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab242140.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Sign up