• Product name

  • Description

    Rabbit polyclonal to HNF1
  • Host species

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Pig
  • Immunogen

    Recombinant fragment corresponding to Human HNF1 aa 35-238.
    Database link: P20823

  • Positive control

    • WB: HepG2 whole cell lysate (ab7900) IHC-P: A549 Xenograft



Our Abpromise guarantee covers the use of ab96777 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 67 kDa.
IHC-P 1/100 - 1/250.
ICC/IF 1/100 - 1/1000.
IP 1/100 - 1/500.


  • Function

    Transcriptional activator that regulates the tissue specific expression of multiple genes, especially in pancreatic islet cells and in liver. Required for the expression of several liver specific genes. Binds to the inverted palindrome 5'-GTTAATNATTAAC-3'.
  • Tissue specificity

  • Involvement in disease

    Defects in HNF1A are a cause of hepatic adenomas familial (HEPAF) [MIM:142330]. Hepatic adenomas are rare benign liver tumors of presumable epithelial origin that develop in an otherwise normal liver. Hepatic adenomas may be single or multiple. They consist of sheets of well-differentiated hepatocytes that contain fat and glycogen and can produce bile. Bile ducts or portal areas are absent. Kupffer cells, if present, are reduced in number and are non-functional. Conditions associated with adenomas are insulin-dependent diabetes mellitus and glycogen storage diseases (types 1 and 3). Note=Bi-allelic inactivation of HNF1A, whether sporadic or associated with MODY3, may be an early step in the developmant of some hepatocellular carcinomas.
    Defects in HNF1A are the cause of maturity-onset diabetes of the young type 3 (MODY3) [MIM:600496]; also symbolized MODY-3. MODY is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease.
    Defects in HNF1A are the cause of susceptibility to diabetes mellitus insulin-dependent type 20 (IDDM20) [MIM:612520]. IDDM20 is a multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical fetaures are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These features can result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels.
  • Sequence similarities

    Belongs to the HNF1 homeobox family.
    Contains 1 homeobox DNA-binding domain.
  • Cellular localization

  • Information by UniProt
  • Database links

  • Alternative names

    • Albumin proximal factor antibody
    • Hepatic nuclear factor 1 alpha antibody
    • Hepatic nuclear factor 1 antibody
    • Hepatic transcription factor 1 alpha antibody
    • Hepatic transcription factor 1 antibody
    • Hepatocyte nuclear factor 1-alpha antibody
    • HNF 1 antibody
    • HNF 1A antibody
    • HNF-1-alpha antibody
    • HNF-1A antibody
    • hnf1a antibody
    • HNF1A_HUMAN antibody
    • Interferon production regulator factor antibody
    • LF B1 antibody
    • LF B1 hepatic nuclear factor antibody
    • LFB 1 antibody
    • LFB1 antibody
    • LFB1 hepatic nuclear factor antibody
    • Liver specific transcription factor LF B1 antibody
    • Liver specific transcription factor LFB1 antibody
    • Liver-specific transcription factor LF-B1 antibody
    • Maturity onset diabetes of the young 3 antibody
    • MODY 3 antibody
    • MODY3 antibody
    • TCF 1 antibody
    • TCF-1 antibody
    • TCF1 antibody
    • Transcription factor 1 antibody
    • Transcription factor 1 hepatic antibody
    see all


  • Anti-HNF1 antibody (ab96777) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg

    Predicted band size: 67 kDa

    7.5% SDS PAGE
  • ab96777, at a 1/100 dilution, staining HNF1 in paraffin embedded A549 Xenograft by Immunohistochemistry.
  • ab96777 staining HNF1 in HeLa cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% paraformaldehyde at room temperature for 15 minutes. Samples were incubated with primary antibody (1/500) and stained green, alpha tubulin stained red and Hoecsht 33342 stained blue


This product has been referenced in:

  • Hiratsuka K  et al. Induction of human pluripotent stem cells into kidney tissues by synthetic mRNAs encoding transcription factors. Sci Rep 9:913 (2019). Read more (PubMed: 30696889) »
  • Ni Q  et al. Deletion of HNF1a in hepatocytes results in fatty liver-related hepatocellular carcinoma in mice. FEBS Lett 591:1947-1957 (2017). Read more (PubMed: 28547778) »
See all 5 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A


Vielen Dank für Ihre Anfrage.

In der Tat bezieht sich das angegebene Molekulargewicht von 67 kDa auf den SwissProt-Eintrag von HNF1 (P20823), der nur den vorhandenen Proteinanteil und keine Modifikationen berücksichtigt. Das unprozessierte HNF1 ergibt jedoch eine Bande bei ca. 95 kDa, und das vollständig post-translational modifizierte Protein läuft im Western blot bei etwa 116 kDa oder sogar mehr, wie auch Testreihen im Labor bestätigen. Die genauen Ursachen für die Größenunterschiede sind nicht bekannt, jedoch wird die 95 kDa-Bande vielfach in der Literatur zitiert.

Wir versuchen nach Möglichkeit alle wichtigen Daten über unsere Produkte an unsere Kunden weiterzugeben und ich möchte mich dafür entschuldigen, dass dies in diesem Fall nicht möglich ist. Ich hoffe, diese Informationen und eine weitere Literatursuche helfen Ihnen dennoch weiter. Bitte zögern Sie nicht, sich wieder bei uns zu melden, falls Sie weitere Fragen haben.

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La secuencia del inmunógeno para este anticuerpo (ab96777) muestra un porcentaje de homología del 62% con la unidad HNF1 beta, por lo que es improbable que haya reactividad cruzada con esta isoforma. Espero que esta información os sea de utilidad. No dudéis en contactarnos para cualquier otra duda o sugerencia.

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