Product nameAnti-HNF6 antibody
See all HNF6 primary antibodies
DescriptionRabbit polyclonal to HNF6
Tested applicationsSuitable for: IHC-P, WB, ELISAmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Horse, Cow, Cat, Dog, Pig
- Purchase matching WB positive control:Recombinant Human HNF6 protein
- Jurkat cell lysate
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab83564 is purified by peptide affinity chromatography method.
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
Our Abpromise guarantee covers the use of ab83564 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 µg/ml.|
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 51 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|ELISA||Use a concentration of 0.2 - 1 µg/ml.|
FunctionTranscriptional activator. Binds the consensus sequence 5'-DHWATTGAYTWWD-3' on a variety of gene promoters such as those of HNF3B and TTR. Important for liver genes transcription.
Tissue specificityHighly expressed in liver; lower expression in testis and skin.
Sequence similaritiesBelongs to the CUT homeobox family.
Contains 1 CUT DNA-binding domain.
Contains 1 homeobox DNA-binding domain.
- Information by UniProt
- hepatocyte nuclear factor 6 alpha antibody
- Hepatocyte nuclear factor 6 antibody
- HNF 6 antibody
Anti-HNF6 antibody (ab83564) at 1 µg/ml (in 5% skim milk / PBS buffer) + Jurkat cell lysate at 10 µg
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 51 kDa
Observed band size: 51 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human brain, cerebellum tissue labelling HNF6 with ab83564 at 5.0µg/ml.
This product has been referenced in:
- Yuan XW et al. Hepatocyte Nuclear Factor 6 Suppresses the Migration and Invasive Growth of Lung Cancer Cells through p53 and the Inhibition of Epithelial-Mesenchymal Transition. J Biol Chem 288:31206-16 (2013). WB ; Human . Read more (PubMed: 24022481) »