- Datasheet
- Specific References
- Protocols
Overview
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Product nameAnti-HNF6 antibody
See all HNF6 primary antibodies -
DescriptionRabbit polyclonal to HNF6
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Host speciesRabbit
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Tested applicationsSuitable for: WBmore details
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Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Zebrafish
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Immunogen
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human HNF6.
(Peptide available as ab90166.)
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Positive control
- Purchase matching WB positive control:Recombinant Human HNF6 protein
- This antibody gave a positive signal in human liver tissue lysate.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab89623 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa). |
Target
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FunctionTranscriptional activator. Binds the consensus sequence 5'-DHWATTGAYTWWD-3' on a variety of gene promoters such as those of HNF3B and TTR. Important for liver genes transcription.
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Tissue specificityHighly expressed in liver; lower expression in testis and skin.
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Sequence similaritiesBelongs to the CUT homeobox family.
Contains 1 CUT DNA-binding domain.
Contains 1 homeobox DNA-binding domain. -
Cellular localizationNucleus.
- Information by UniProt
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Database links
- Entrez Gene: 3175 Human
- Entrez Gene: 15379 Mouse
- Entrez Gene: 25231 Rat
- Omim: 604164 Human
- SwissProt: Q9UBC0 Human
- SwissProt: O08755 Mouse
- SwissProt: P70512 Rat
- Unigene: 658573 Human
see all -
Alternative names
- hepatocyte nuclear factor 6 alpha antibody
- Hepatocyte nuclear factor 6 antibody
- HNF 6 antibody
see all
Images
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Western blot - Anti-HNF6 antibody (ab89623)Anti-HNF6 antibody (ab89623) at 1 µg/ml + Human liver tissue lysate - total protein (ab29889) at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 51 kDa
Observed band size: 51 kDa
Additional bands at: 29 kDa, 38 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 4 minutes
Protocols
References
ab89623 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"