Anti-HNF6 antibody (ab89623)
Key features and details
- Rabbit polyclonal to HNF6
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-HNF6 antibody
See all HNF6 primary antibodies -
Description
Rabbit polyclonal to HNF6 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Zebrafish
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Immunogen
Synthetic peptide corresponding to Human HNF6 aa 1-100 (N terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab90166) -
Positive control
- Purchase matching WB positive control:Recombinant Human HNF6 protein
- This antibody gave a positive signal in human liver tissue lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab89623 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa).
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| Notes |
|---|
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa). |
Target
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Function
Transcriptional activator. Binds the consensus sequence 5'-DHWATTGAYTWWD-3' on a variety of gene promoters such as those of HNF3B and TTR. Important for liver genes transcription. -
Tissue specificity
Highly expressed in liver; lower expression in testis and skin. -
Sequence similarities
Belongs to the CUT homeobox family.
Contains 1 CUT DNA-binding domain.
Contains 1 homeobox DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 3175 Human
- Entrez Gene: 15379 Mouse
- Entrez Gene: 25231 Rat
- Omim: 604164 Human
- SwissProt: Q9UBC0 Human
- SwissProt: O08755 Mouse
- SwissProt: P70512 Rat
- Unigene: 658573 Human
see all -
Alternative names
- hepatocyte nuclear factor 6 alpha antibody
- Hepatocyte nuclear factor 6 antibody
- HNF 6 antibody
see all
Images
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Western blot - Anti-HNF6 antibody (ab89623)Anti-HNF6 antibody (ab89623) at 1 µg/ml + Human liver tissue lysate - total protein (ab29889) at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 51 kDa
Observed band size: 51 kDa
Additional bands at: 29 kDa, 38 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 4 minutes
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab89623 has not yet been referenced specifically in any publications.