Product nameAnti-HNF6 antibody [EPR14976]
See all HNF6 primary antibodies
DescriptionRabbit monoclonal [EPR14976] to HNF6
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Recombinant fragment within Human HNF6 aa 1-100. The exact sequence is proprietary.
Database link: Q9UBC0
- WB: Human fetal liver, human and rat liver tissue lysates; HepG2 and Hepa1-6 whole cell lysates.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab186743 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa).|
FunctionTranscriptional activator. Binds the consensus sequence 5'-DHWATTGAYTWWD-3' on a variety of gene promoters such as those of HNF3B and TTR. Important for liver genes transcription.
Tissue specificityHighly expressed in liver; lower expression in testis and skin.
Sequence similaritiesBelongs to the CUT homeobox family.
Contains 1 CUT DNA-binding domain.
Contains 1 homeobox DNA-binding domain.
- Information by UniProt
- hepatocyte nuclear factor 6 alpha antibody
- Hepatocyte nuclear factor 6 antibody
- HNF 6 antibody
All lanes : Anti-HNF6 antibody [EPR14976] (ab186743) at 1/200 dilution
Lane 1 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates at 20 µg
Lane 2 : Hepa1-6 (Mouse hepatoma epithelial cell) whole cell lysates at 15 µg
Lane 3 : Rat liver tissue lysates at 15 µg
Lane 4 : Human liver tissue lysates at 15 µg
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 51 kDa
Observed band size: 51 kDa
Exposure time: 100 seconds
Blocking/Diluting buffer and concentration 5% NFDM/TBST
This antibody shows low affinity on liver samples which should be positive according to PMID 27445394.
ab186743 has not yet been referenced specifically in any publications.