Anti-hnRNP A1 antibody [9H10] (ab5832)
Key features and details
- Mouse monoclonal [9H10] to hnRNP A1
- Suitable for: Flow Cyt, IHC-P, ELISA, WB, IP, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG2b
Overview
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Product name
Anti-hnRNP A1 antibody [9H10]
See all hnRNP A1 primary antibodies -
Description
Mouse monoclonal [9H10] to hnRNP A1 -
Host species
Mouse -
Tested applications
Suitable for: Flow Cyt, IHC-P, ELISA, WB, IP, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Full length hnRNPA1 native protein (partially purified) from HeLa cells (Human).
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein A purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
9H10 -
Myeloma
Sp2/0 -
Isotype
IgG2b -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab5832 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt |
Use 1µg for 106 cells.
ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
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IHC-P |
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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ELISA |
Use at an assay dependent concentration.
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WB | (3) |
Use at an assay dependent concentration. Detects a band of approximately 34 kDa (predicted molecular weight: 38 kDa).
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IP |
Use at an assay dependent concentration. This antibody does not IP the hnRNP complex.
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ICC/IF | (2) |
Use at an assay dependent concentration.
See Abreview (April 2, 2007). |
Notes |
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Flow Cyt
Use 1µg for 106 cells. ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
ELISA
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Detects a band of approximately 34 kDa (predicted molecular weight: 38 kDa). |
IP
Use at an assay dependent concentration. This antibody does not IP the hnRNP complex. |
ICC/IF
Use at an assay dependent concentration. See Abreview (April 2, 2007). |
Target
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Function
Involved in the packaging of pre-mRNA into hnRNP particles, transport of poly(A) mRNA from the nucleus to the cytoplasm and may modulate splice site selection. May play a role in HCV RNA replication. -
Sequence similarities
Contains 2 RRM (RNA recognition motif) domains. -
Post-translational
modificationsArg-194, Arg-206 and Arg-225 are dimethylated, probably to asymmetric dimethylarginine.
Sumoylated. -
Cellular localization
Nucleus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Shuttles continuously between the nucleus and the cytoplasm along with mRNA. Component of ribonucleosomes. In the course of viral infection, colocalizes with HCV NS5B at speckles in the cytoplasm in a HCV-replication dependent manner. - Information by UniProt
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Database links
- Entrez Gene: 3178 Human
- Entrez Gene: 15382 Mouse
- Omim: 164017 Human
- SwissProt: P09651 Human
- SwissProt: P49312 Mouse
- Unigene: 546261 Human
- Unigene: 655424 Human
- Unigene: 237064 Mouse
see all -
Alternative names
- HNRNPA 1 antibody
- Helix destabilizing protein antibody
- Helix-destabilizing protein antibody
see all
Images
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All lanes : Anti-hnRNP A1 antibody [9H10] (ab5832) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 38 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?
Additional bands at: 43 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP A1 antibody [9H10] (ab5832)Ab5832 staining human lung. Staining is localized to the nucleus.
Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
Sections were stained using an automated system (Dako PT Link), at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 antigen retrieval buffer, citrate pH 6.0. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required. -
Western blot - Anti-hnRNP A1 antibody [9H10] (ab5832)This image is courtesy of an anonymous Abreview.All lanes : Anti-hnRNP A1 antibody [9H10] (ab5832) at 1/1000 dilution
Lane 1 : Mouse NSC34 whole cell lysate with control siRNA
Lane 2 : Mouse NSC34 whole cell lysate with hnRNP A1 siRNA
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : IRDye® 700DX-conjugated Donkey anti-Mouse IgG at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 38 kDa
Exposure time: 1 minute
Knockdown for 72 hours. -
Overlay histogram showing Jurkat cells stained with ab5832 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab5832, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 4% paraformaldehyde/permeabilized in 0.1% PBS-Tween used under the same conditions.
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hnRNP A1 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Mouse monoclonal to hnRNP A1 (ab5832) and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab5832.
Secondary: Goat polyclonal to mouse IgG light chain specific (HRP) at 1/5000 dilution.
Band: 37kDa: hnRNP A1; 42kDa:We are unsure as to the identity of this extra band.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (44)
ab5832 has been referenced in 44 publications.
- Chen M et al. PRMT5 regulates ovarian follicle development by facilitating Wt1 translation. Elife 10:N/A (2021). PubMed: 34448450
- Möller K et al. Upregulation of the heterogeneous nuclear ribonucleoprotein hnRNPA1 is an independent predictor of early biochemical recurrence in TMPRSS2:ERG fusion-negative prostate cancers. Virchows Arch N/A:N/A (2020). PubMed: 32417965
- Sun G et al. HnRNP A1 - mediated alternative splicing of CCDC50 contributes to cancer progression of clear cell renal cell carcinoma via ZNF395. J Exp Clin Cancer Res 39:116 (2020). PubMed: 32560659
- Zheng Y et al. Heterogeneous Nuclear Ribonucleoprotein A1 (hnRNP A1) and hnRNP A2 Inhibit Splicing to Human Papillomavirus 16 Splice Site SA409 through a UAG-Containing Sequence in the E7 Coding Region. J Virol 94:N/A (2020). PubMed: 32759322
- Noto PB et al. Identification of hnRNP-A1 as a pharmacodynamic biomarker of type I PRMT inhibition in blood and tumor tissues. Sci Rep 10:22155 (2020). PubMed: 33335114