Key features and details
- Rabbit polyclonal to hnRNP A1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-hnRNP A1 antibody
See all hnRNP A1 primary antibodies
DescriptionRabbit polyclonal to hnRNP A1
SpecificityIt detects endogenous levels of total hnRNP A1 protein.
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide from internal sequence of Human hnRNP A1
- Human Small Intestine tissue. 293, Jurkat and COLO205 cell extracts. HeLa cells.
Storage instructionsShipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Store undiluted.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 0.88% Sodium chloride, 50% Glycerol
PBS is without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab131138 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 39 kDa.|
|IHC-P||Use a concentration of 5 µg/ml.|
|ICC/IF||1/100 - 1/500.|
FunctionInvolved in the packaging of pre-mRNA into hnRNP particles, transport of poly(A) mRNA from the nucleus to the cytoplasm and may modulate splice site selection. May play a role in HCV RNA replication.
Sequence similaritiesContains 2 RRM (RNA recognition motif) domains.
modificationsArg-194, Arg-206 and Arg-225 are dimethylated, probably to asymmetric dimethylarginine.
Cellular localizationNucleus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Shuttles continuously between the nucleus and the cytoplasm along with mRNA. Component of ribonucleosomes. In the course of viral infection, colocalizes with HCV NS5B at speckles in the cytoplasm in a HCV-replication dependent manner.
- Information by UniProt
- HNRNPA 1 antibody
- Helix destabilizing protein antibody
- Helix-destabilizing protein antibody
All lanes : Anti-hnRNP A1 antibody (ab131138) at 1/500 dilution
Lane 1 : 293 cell extract
Lane 2 : Jurkat cell extract
Lane 3 : COLO205 cell extract
Lane 4 : 293 cell extract with synthesized peptide
Predicted band size: 39 kDa
Immunohistochemistry of Formalin-Fixed, Paraffin-Embedded Human small intestine tissue labelling hnRNP A1 (isoform A1-A) with ab131138 at 5 µg/ml.
Immunofluorescence analysis of HeLa cells labelling hnRNP A1 (isoform A1-A) with ab131138 at 1/100 dilution. The picture on the right is treated with the synthesized peptide
ab131138 has not yet been referenced specifically in any publications.