Anti-hnRNP A1 antibody (ab137780)

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Overview

  • Product name

    Anti-hnRNP A1 antibody
    See all hnRNP A1 primary antibodies

  • Description

    Rabbit polyclonal to hnRNP A1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IF, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow, Monkey

  • Immunogen

    Recombinant fragment, corresponding to amino acids 1-214 of Human hnRNP A1 (UniProt ID: P09651).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • Mouse brain and 293T, A431, H1299, HeLaS3, Hela, HepG2, Molt4 and Raji cell lysates; HeLa cells; Ca922 xenograft

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab137780 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 39 kDa.
ICC/IF 1/100 - 1/1000.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Involved in the packaging of pre-mRNA into hnRNP particles, transport of poly(A) mRNA from the nucleus to the cytoplasm and may modulate splice site selection. May play a role in HCV RNA replication.

  • Sequence similarities

    Contains 2 RRM (RNA recognition motif) domains.

  • Post-translational
    modifications

    Arg-194, Arg-206 and Arg-225 are dimethylated, probably to asymmetric dimethylarginine.
    Sumoylated.

  • Cellular localization

    Nucleus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Shuttles continuously between the nucleus and the cytoplasm along with mRNA. Component of ribonucleosomes. In the course of viral infection, colocalizes with HCV NS5B at speckles in the cytoplasm in a HCV-replication dependent manner.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • HNRNPA 1 antibody
    • Helix destabilizing protein antibody
    • Helix-destabilizing protein antibody
    • Heterogeneous nuclear ribonucleoprotein A1 antibody
    • Heterogeneous nuclear ribonucleoprotein A1B protein antibody
    • Heterogeneous nuclear ribonucleoprotein B2 protein antibody
    • Heterogeneous nuclear ribonucleoprotein core protein A1 antibody
    • hnRNP A1 antibody
    • hnRNP core protein A1 antibody
    • HNRNPA1 antibody
    • HNRPA1 antibody
    • MGC102835 antibody
    • Nuclear ribonucleoprotein particle A1 protein antibody
    • ROA1_HUMAN antibody
    • Single strand DNA binding protein UP1 antibody
    • Single strand RNA binding protein antibody
    • Single-strand RNA-binding protein antibody
    see all

Images

  • Western blot - Anti-hnRNP A1 antibody (ab137780)
    Western blot - Anti-hnRNP A1 antibody (ab137780)
    All lanes : Anti-hnRNP A1 antibody (ab137780) at 1/3000 dilution

    Lane 1 : WT 293T cell extracts
    Lane 2 : hnRNP A1 Knockout 293T cell extracts

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Predicted band size: 39 kDa

  • Western blot - Anti-hnRNP A1 antibody (ab137780)
    Western blot - Anti-hnRNP A1 antibody (ab137780)
    All lanes : Anti-hnRNP A1 antibody (ab137780) at 1/500 dilution

    Lane 1 : H1299 whole cell lysate
    Lane 2 : HelaS3 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 39 kDa



    12% SDS PAGE
  • Western blot - Anti-hnRNP A1 antibody (ab137780)
    Western blot - Anti-hnRNP A1 antibody (ab137780)
    Anti-hnRNP A1 antibody (ab137780) at 1/1000 dilution + Mouse brain cell lysate at 50 µg

    Predicted band size: 39 kDa



    10% SDS PAGE
  • Immunocytochemistry/ Immunofluorescence - Anti-hnRNP A1 antibody (ab137780)
    Immunocytochemistry/ Immunofluorescence - Anti-hnRNP A1 antibody (ab137780)
    Immunofluorescence analysis of paraformaldehyde fixed HeLa cells labeling hnRNP A1 with ab137780 at a 1/200 dilution. The lower image (labeled '2') was merged with DNA probe.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP A1 antibody (ab137780)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP A1 antibody (ab137780)
    Immunohistochemical analysis of paraffin embedded Ca922 xenograft, labelling hnRNP A1 using ab137780 at a 1/100 dilution.

References

This product has been referenced in:

  • Hasan AA  et al. Mechanisms of GLP-1 receptor-independent renoprotective effects of the dipeptidyl peptidase type 4 inhibitor linagliptin in GLP-1 receptor knockout mice with 5/6 nephrectomy. Kidney Int 95:1373-1388 (2019). Read more (PubMed: 30979564) »
  • Lin YJ  et al. P-coumaric acid regulates exon 12 splicing of the ATP7B gene by modulating hnRNP A1 protein expressions. Biomedicine (Taipei) 5:10 (2015). Read more (PubMed: 26048696) »
See all 2 Publications for this product

Publishing research using ab137780? Please let us know so that we can cite the reference in this datasheet.

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