Anti-hnRNP A1 antibody (ab137780)
Key features and details
- Rabbit polyclonal to hnRNP A1
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-hnRNP A1 antibody
See all hnRNP A1 primary antibodies -
Description
Rabbit polyclonal to hnRNP A1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P HumanWB MouseHuman
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Immunogen
Recombinant fragment, corresponding to amino acids 1-214 of Human hnRNP A1 (UniProt ID: P09651).
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Positive control
- Mouse brain and 293T, A431, H1299, HeLaS3, Hela, HepG2, Molt4 and Raji cell lysates; HeLa cells; Ca922 xenograft
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab137780 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| ICC/IF |
Human
|
| IHC-P |
Human
|
| WB |
Mouse
Human
|
| All applications |
Rat
Cow
Monkey
|
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 39 kDa.
|
|
| ICC/IF |
1/100 - 1/1000.
|
|
| IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| Notes |
|---|
|
WB
1/500 - 1/3000. Predicted molecular weight: 39 kDa. |
|
ICC/IF
1/100 - 1/1000. |
|
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Involved in the packaging of pre-mRNA into hnRNP particles, transport of poly(A) mRNA from the nucleus to the cytoplasm and may modulate splice site selection. May play a role in HCV RNA replication. -
Sequence similarities
Contains 2 RRM (RNA recognition motif) domains. -
Post-translational
modificationsArg-194, Arg-206 and Arg-225 are dimethylated, probably to asymmetric dimethylarginine.
Sumoylated. -
Cellular localization
Nucleus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Shuttles continuously between the nucleus and the cytoplasm along with mRNA. Component of ribonucleosomes. In the course of viral infection, colocalizes with HCV NS5B at speckles in the cytoplasm in a HCV-replication dependent manner. - Information by UniProt
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Database links
- Entrez Gene: 3178 Human
- Entrez Gene: 15382 Mouse
- Entrez Gene: 29578 Rat
- Omim: 164017 Human
- SwissProt: P09867 Cow
- SwissProt: P09651 Human
- SwissProt: P49312 Mouse
- SwissProt: P04256 Rat
see all -
Alternative names
- HNRNPA 1 antibody
- Helix destabilizing protein antibody
- Helix-destabilizing protein antibody
see all
Images
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Western blot - Anti-hnRNP A1 antibody (ab137780)All lanes : Anti-hnRNP A1 antibody (ab137780) at 1/3000 dilution
Lane 1 : WT 293T cell extracts
Lane 2 : hnRNP A1 Knockout 293T cell extracts
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 39 kDa -
Western blot - Anti-hnRNP A1 antibody (ab137780)All lanes : Anti-hnRNP A1 antibody (ab137780) at 1/500 dilution
Lane 1 : H1299 whole cell lysate
Lane 2 : HelaS3 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 39 kDa
12% SDS PAGE -
Western blot - Anti-hnRNP A1 antibody (ab137780)Anti-hnRNP A1 antibody (ab137780) at 1/1000 dilution + Mouse brain cell lysate at 50 µg
Predicted band size: 39 kDa
10% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-hnRNP A1 antibody (ab137780)Immunofluorescence analysis of paraformaldehyde fixed HeLa cells labeling hnRNP A1 with ab137780 at a 1/200 dilution. The lower image (labeled '2') was merged with DNA probe. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP A1 antibody (ab137780)Immunohistochemical analysis of paraffin embedded Ca922 xenograft, labelling hnRNP A1 using ab137780 at a 1/100 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab137780 has been referenced in 2 publications.
- Hasan AA et al. Mechanisms of GLP-1 receptor-independent renoprotective effects of the dipeptidyl peptidase type 4 inhibitor linagliptin in GLP-1 receptor knockout mice with 5/6 nephrectomy. Kidney Int 95:1373-1388 (2019). PubMed: 30979564
- Lin YJ et al. P-coumaric acid regulates exon 12 splicing of the ATP7B gene by modulating hnRNP A1 protein expressions. Biomedicine (Taipei) 5:10 (2015). PubMed: 26048696