Key features and details
- Rabbit polyclonal to hnRNP A1
- Suitable for: WB, IHC-P
- Reacts with: Human, Pig
- Isotype: IgG
Product nameAnti-hnRNP A1 antibody
See all hnRNP A1 primary antibodies
DescriptionRabbit polyclonal to hnRNP A1
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human, Pig
Recombinant full length protein (His-T7-tag) corresponding to Human hnRNP A1 aa 2-372. Expressed in E.coli. N-terminal tags.
SKSESPKEPEQLRKLFIGGLSFETTDESLRSHFEQWGTLTDCVVMRDPNT KRSRGFGFVTYATVEEVDAAMNARPHKVDGRVVEPKRAVSREDSQRPGAH LTVKKIFVGGIKEDTEEHHLRDYFEQYGKIEVIEIMTDRGSGKKRGFAFV TFDDHDSVDKIVIQKYHTVNGHNCEVRKALSKQEMASASSSQRGRSGSGN FGGGRGGGFGGNDNFGRGGNFSGRGGFGGSRGGGGYGGSGDGYNGFGNDG GYGGGGPGYSGGSRGYGSGGQGYGNQGSGYGGSGSYDSYNNGGGGGFGGG SGSNFGGGGSYNDFGNYNNQSSNFGPMKGGNFGGRSSGPYGGGGQYFAKP RNQGGYGGSSSSSSYGSGRRF
Database link: P09651-1
- WB: Recombinant human hnRNP A1 protein. HeLa, Jurkat and MCF7 cell lysates. Pig intestine tissue lysate. IHC-P: Human prostate cancer, skin cancer, glioma, brain, breast cancer and lung cancer tissue.
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, PBS
Concentration information loading...
PurityProtein A purified
Purification notesAntigen-specific affinity chromatography followed by Protein A affinity chromatography.
Our Abpromise guarantee covers the use of ab232824 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 39 kDa.|
|IHC-P||Use a concentration of 5 - 20 µg/ml.|
FunctionInvolved in the packaging of pre-mRNA into hnRNP particles, transport of poly(A) mRNA from the nucleus to the cytoplasm and may modulate splice site selection. May play a role in HCV RNA replication.
Sequence similaritiesContains 2 RRM (RNA recognition motif) domains.
modificationsArg-194, Arg-206 and Arg-225 are dimethylated, probably to asymmetric dimethylarginine.
Cellular localizationNucleus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Shuttles continuously between the nucleus and the cytoplasm along with mRNA. Component of ribonucleosomes. In the course of viral infection, colocalizes with HCV NS5B at speckles in the cytoplasm in a HCV-replication dependent manner.
- Information by UniProt
- HNRNPA 1 antibody
- Helix destabilizing protein antibody
- Helix-destabilizing protein antibody
All lanes : Anti-hnRNP A1 antibody (ab232824) at 3 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 2 : Pig intestine tissue lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate
Lane 4 : MCF7 (human breast adenocarcinoma cell line) cell lysate
All lanes : HRP-linked Guinea pig anti-Rabbit at 1/2000 dilution
Predicted band size: 39 kDa
Formalin-fixed, paraffin-embedded human skin cancer tissue stained for hnRNP A1 with ab232824 at 10 µg/ml in immunohistochemical analysis. DAB staining.
Formalin-fixed, paraffin-embedded human breast cancer tissue stained for hnRNP A1 with ab232824 at 10 µg/ml in immunohistochemical analysis. DAB staining.
Formalin-fixed, paraffin-embedded human prostate cancer tissue stained for hnRNP A1 with ab232824 at 10 µg/ml in immunohistochemical analysis. DAB staining.
Formalin-fixed, paraffin-embedded human brain tissue stained for hnRNP A1 with ab232824 at 10 µg/ml in immunohistochemical analysis. DAB staining.
Formalin-fixed, paraffin-embedded human glioma tissue stained for hnRNP A1 with ab232824 at 10 µg/ml in immunohistochemical analysis. DAB staining.
Formalin-fixed, paraffin-embedded human lung cancer tissue stained for hnRNP A1 with ab232824 at 10 µg/ml in immunohistochemical analysis. DAB staining.
Anti-hnRNP A1 antibody (ab232824) at 2 µg/ml + Recombinant human hnRNP A1 protein
Predicted band size: 39 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab232824 has not yet been referenced specifically in any publications.