Validated using a knockout cell line
Recombinant
RabMAb

Recombinant Anti-hnRNP A1 antibody [EPR12768] (ab177152)

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Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12768] to hnRNP A1
  • Suitable for: IHC-P, WB, ICC/IF, Flow Cyt
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-hnRNP A1 antibody [EPR12768]
    See all hnRNP A1 primary antibodies

  • Description

    Rabbit monoclonal [EPR12768] to hnRNP A1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WB, ICC/IF, Flow Cytmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human hnRNP A1 aa 300 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: P09651

  • Positive control

    • MCF-7, HepG2, K562, HEK-293T, HeLa and Jurkat whole cell lysate (ab7899). Human breast carcinoma and clear cell carcinoma tissue. HepG2 cells. Permeabilized Jurkat cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.5% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR12768

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab177152 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB 1/1000 - 1/10000. Detects a band of approximately 34 kDa (predicted molecular weight: 38 kDa).
ICC/IF 1/100 - 1/250.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • Application notes
    Is unsuitable for IP.

    • Target

    • Function

      Involved in the packaging of pre-mRNA into hnRNP particles, transport of poly(A) mRNA from the nucleus to the cytoplasm and may modulate splice site selection. May play a role in HCV RNA replication.

    • Sequence similarities

      Contains 2 RRM (RNA recognition motif) domains.

    • Post-translational
      modifications

      Arg-194, Arg-206 and Arg-225 are dimethylated, probably to asymmetric dimethylarginine.
      Sumoylated.

    • Cellular localization

      Nucleus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Shuttles continuously between the nucleus and the cytoplasm along with mRNA. Component of ribonucleosomes. In the course of viral infection, colocalizes with HCV NS5B at speckles in the cytoplasm in a HCV-replication dependent manner.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • HNRNPA 1 antibody
      • Helix destabilizing protein antibody
      • Helix-destabilizing protein antibody
      • Heterogeneous nuclear ribonucleoprotein A1 antibody
      • Heterogeneous nuclear ribonucleoprotein A1B protein antibody
      • Heterogeneous nuclear ribonucleoprotein B2 protein antibody
      • Heterogeneous nuclear ribonucleoprotein core protein A1 antibody
      • hnRNP A1 antibody
      • hnRNP core protein A1 antibody
      • HNRNPA1 antibody
      • HNRPA1 antibody
      • MGC102835 antibody
      • Nuclear ribonucleoprotein particle A1 protein antibody
      • ROA1_HUMAN antibody
      • Single strand DNA binding protein UP1 antibody
      • Single strand RNA binding protein antibody
      • Single-strand RNA-binding protein antibody
      see all

    Images

    • Western blot - Anti-hnRNP A1 antibody [EPR12768] (ab177152)
      Western blot - Anti-hnRNP A1 antibody [EPR12768] (ab177152)
      All lanes : Anti-hnRNP A1 antibody [EPR12768] (ab177152) at 1/1000 dilution

      Lane 1 : Wild-type HEK293T cell lysate
      Lane 2 : hnRNP A1 knockout HEK293T cell lysate
      Lane 3 : HeLa cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

      Predicted band size: 38 kDa
      Observed band size: 37 kDa
      why is the actual band size different from the predicted?



      Lanes 1-3: Merged signal (red and green). Green - ab177152 observed at 37 kDa. Red - loading control ab8245 observed at 36 kDa.

      ab177152 Anti-hnRNP A1 antibody [EPR12768] was shown to specifically react with hnRNP A1 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266193 (knockout cell lysate ab256942) was used. Wild-type and hnRNP A1 knockout samples were subjected to SDS-PAGE. ab177152 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

       

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP A1 antibody [EPR12768] (ab177152)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP A1 antibody [EPR12768] (ab177152)

      Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling hnRNP A1 with ab177152 at 1/50 dilution.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Western blot - Anti-hnRNP A1 antibody [EPR12768] (ab177152)
      Western blot - Anti-hnRNP A1 antibody [EPR12768] (ab177152)
      All lanes : Anti-hnRNP A1 antibody [EPR12768] (ab177152) at 1/1000 dilution

      Lane 1 : MCF7 cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : K562 cell lysate
      Lane 4 : HeLa cell lysate
      Lane 5 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 38 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-hnRNP A1 antibody [EPR12768] (ab177152)
      Immunocytochemistry/ Immunofluorescence - Anti-hnRNP A1 antibody [EPR12768] (ab177152)

      Immunofluorescent analysis of HepG2 cells labeling hnRNP A1 with ab177152 at 1/100 dilution.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP A1 antibody [EPR12768] (ab177152)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP A1 antibody [EPR12768] (ab177152)

      Immunohistochemical analysis of paraffin-embedded Human clear cell carcinoma tissue labeling hnRNP A1 with ab177152 at 1/50 dilution.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Flow Cytometry - Anti-hnRNP A1 antibody [EPR12768] (ab177152)
      Flow Cytometry - Anti-hnRNP A1 antibody [EPR12768] (ab177152)

      Flow cytometric analysis of permeabilized Jurkat cells labeling hnRNP A1 with ab177152 at 1/10 dilution (red), or a rabbit IgG (negative) (green).

    References (4)

    Publishing research using ab177152? Please let us know so that we can cite the reference in this datasheet.

    ab177152 has been referenced in 4 publications.

    • Ke RS  et al. Prognostic value and oncogene function of heterogeneous nuclear ribonucleoprotein A1 overexpression in HBV-related hepatocellular carcinoma. Int J Biol Macromol 129:140-151 (2019). PubMed: 30731163
    • Jia Q  et al. HNRNPA1-mediated 3' UTR length changes of HN1 contributes to cancer- and senescence-associated phenotypes. Aging (Albany NY) 11:4407-4437 (2019). PubMed: 31257225
    • Ke R  et al. Prognostic value of heterogeneous ribonucleoprotein A1 expression and inflammatory indicators for patients with surgically resected hepatocellular carcinoma: Perspectives from a high occurrence area of hepatocellular carcinoma in China. Oncol Lett 16:3746-3756 (2018). PubMed: 30127985
    • Leong SY  et al. The role of Misshapen NCK-related kinase (MINK), a novel Ste20 family kinase, in the IRES-mediated protein translation of human enterovirus 71. PLoS Pathog 11:e1004686 (2015). PubMed: 25747578

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