Overview

  • Product name
    Anti-hnRNP A1 antibody [EPR12768]
    See all hnRNP A1 primary antibodies
  • Description
    Rabbit monoclonal [EPR12768] to hnRNP A1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, WB, ICC/IF, Flow Cytmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human hnRNP A1 aa 300 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: P09651

  • Positive control
    • MCF-7, HepG2, K562, HeLa and Jurkat cell lysates. Human breast carcinoma and clear cell carcinoma tissue. HepG2 cells. Permeabilized Jurkat cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.5% BSA
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    EPR12768
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab177152 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB 1/1000 - 1/10000. Detects a band of approximately 34 kDa (predicted molecular weight: 38 kDa).
ICC/IF 1/100 - 1/250.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
  • Application notes
    Is unsuitable for IP.

    • Target

    • Function
      Involved in the packaging of pre-mRNA into hnRNP particles, transport of poly(A) mRNA from the nucleus to the cytoplasm and may modulate splice site selection. May play a role in HCV RNA replication.
    • Sequence similarities
      Contains 2 RRM (RNA recognition motif) domains.
    • Post-translational
      modifications
      Arg-194, Arg-206 and Arg-225 are dimethylated, probably to asymmetric dimethylarginine.
      Sumoylated.
    • Cellular localization
      Nucleus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Shuttles continuously between the nucleus and the cytoplasm along with mRNA. Component of ribonucleosomes. In the course of viral infection, colocalizes with HCV NS5B at speckles in the cytoplasm in a HCV-replication dependent manner.
    • Information by UniProt
    • Database links
      see all
    • Alternative names
      • HNRNPA 1 antibody
      • Helix destabilizing protein antibody
      • Helix-destabilizing protein antibody
      • Heterogeneous nuclear ribonucleoprotein A1 antibody
      • Heterogeneous nuclear ribonucleoprotein A1B protein antibody
      • Heterogeneous nuclear ribonucleoprotein B2 protein antibody
      • Heterogeneous nuclear ribonucleoprotein core protein A1 antibody
      • hnRNP A1 antibody
      • hnRNP core protein A1 antibody
      • HNRNPA1 antibody
      • HNRPA1 antibody
      • MGC102835 antibody
      • Nuclear ribonucleoprotein particle A1 protein antibody
      • ROA1_HUMAN antibody
      • Single strand DNA binding protein UP1 antibody
      • Single strand RNA binding protein antibody
      • Single-strand RNA-binding protein antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP A1 antibody [EPR12768] (ab177152)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP A1 antibody [EPR12768] (ab177152)

      Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling hnRNP A1 with ab177152 at 1/50 dilution.

    • Immunocytochemistry/ Immunofluorescence - Anti-hnRNP A1 antibody [EPR12768] (ab177152)
      Immunocytochemistry/ Immunofluorescence - Anti-hnRNP A1 antibody [EPR12768] (ab177152)

      Immunofluorescent analysis of HepG2 cells labeling hnRNP A1 with ab177152 at 1/100 dilution.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP A1 antibody [EPR12768] (ab177152)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP A1 antibody [EPR12768] (ab177152)

      Immunohistochemical analysis of paraffin-embedded Human clear cell carcinoma tissue labeling hnRNP A1 with ab177152 at 1/50 dilution.

    • Flow Cytometry - Anti-hnRNP A1 antibody [EPR12768] (ab177152)
      Flow Cytometry - Anti-hnRNP A1 antibody [EPR12768] (ab177152)

      Flow cytometric analysis of permeabilized Jurkat cells labeling hnRNP A1 with ab177152 at 1/10 dilution (red), or a rabbit IgG (negative) (green).

    • Western blot - Anti-hnRNP A1 antibody [EPR12768] (ab177152)
      Western blot - Anti-hnRNP A1 antibody [EPR12768] (ab177152)
      All lanes : Anti-hnRNP A1 antibody [EPR12768] (ab177152) at 1/1000 dilution

      Lane 1 : MCF7 cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : K562 cell lysate
      Lane 4 : HeLa cell lysate
      Lane 5 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 38 kDa

    References

    This product has been referenced in:
    • Leong SY  et al. The role of Misshapen NCK-related kinase (MINK), a novel Ste20 family kinase, in the IRES-mediated protein translation of human enterovirus 71. PLoS Pathog 11:e1004686 (2015). Read more (PubMed: 25747578) »

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