Recombinant Anti-hnRNP A1 antibody [EPR12768] (ab177152)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12768] to hnRNP A1
- Suitable for: IHC-P, WB, ICC/IF, Flow Cyt
- Knockout validated
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-hnRNP A1 antibody [EPR12768]
See all hnRNP A1 primary antibodies -
Description
Rabbit monoclonal [EPR12768] to hnRNP A1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IF, Flow Cytmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human hnRNP A1 aa 300 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: P09651 -
Positive control
- MCF-7, HepG2, K562, HEK-293T, HeLa and Jurkat whole cell lysate (ab7899). Human breast carcinoma and clear cell carcinoma tissue. HepG2 cells. Permeabilized Jurkat cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR12768 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab177152 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P | 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
WB | 1/1000 - 1/10000. Detects a band of approximately 34 kDa (predicted molecular weight: 38 kDa). | |
ICC/IF | 1/100 - 1/250. | |
Flow Cyt | 1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Target
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Function
Involved in the packaging of pre-mRNA into hnRNP particles, transport of poly(A) mRNA from the nucleus to the cytoplasm and may modulate splice site selection. May play a role in HCV RNA replication. -
Sequence similarities
Contains 2 RRM (RNA recognition motif) domains. -
Post-translational
modificationsArg-194, Arg-206 and Arg-225 are dimethylated, probably to asymmetric dimethylarginine.
Sumoylated. -
Cellular localization
Nucleus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Shuttles continuously between the nucleus and the cytoplasm along with mRNA. Component of ribonucleosomes. In the course of viral infection, colocalizes with HCV NS5B at speckles in the cytoplasm in a HCV-replication dependent manner. - Information by UniProt
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Database links
- Entrez Gene: 3178 Human
- Entrez Gene: 15382 Mouse
- Entrez Gene: 29578 Rat
- Omim: 164017 Human
- SwissProt: P09651 Human
- SwissProt: P49312 Mouse
- SwissProt: P04256 Rat
- Unigene: 546261 Human
see all -
Alternative names
- HNRNPA 1 antibody
- Helix destabilizing protein antibody
- Helix-destabilizing protein antibody
see all
Images
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All lanes : Anti-hnRNP A1 antibody [EPR12768] (ab177152) at 1/1000 dilution
Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : hnRNP A1 knockout HEK293T cell lysate
Lane 3 : HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 38 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?Lanes 1-3: Merged signal (red and green). Green - ab177152 observed at 37 kDa. Red - loading control ab8245 observed at 36 kDa.
ab177152 Anti-hnRNP A1 antibody [EPR12768] was shown to specifically react with hnRNP A1 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266193 (knockout cell lysate ab256942) was used. Wild-type and hnRNP A1 knockout samples were subjected to SDS-PAGE. ab177152 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP A1 antibody [EPR12768] (ab177152)
Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling hnRNP A1 with ab177152 at 1/50 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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All lanes : Anti-hnRNP A1 antibody [EPR12768] (ab177152) at 1/1000 dilution
Lane 1 : MCF7 cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : K562 cell lysate
Lane 4 : HeLa cell lysate
Lane 5 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 38 kDa -
Immunofluorescent analysis of HepG2 cells labeling hnRNP A1 with ab177152 at 1/100 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP A1 antibody [EPR12768] (ab177152)
Immunohistochemical analysis of paraffin-embedded Human clear cell carcinoma tissue labeling hnRNP A1 with ab177152 at 1/50 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Flow cytometric analysis of permeabilized Jurkat cells labeling hnRNP A1 with ab177152 at 1/10 dilution (red), or a rabbit IgG (negative) (green).
Protocols
Datasheets and documents
References (5)
ab177152 has been referenced in 5 publications.
- Ke RS et al. Prognostic value and oncogene function of heterogeneous nuclear ribonucleoprotein A1 overexpression in HBV-related hepatocellular carcinoma. Int J Biol Macromol 129:140-151 (2019). PubMed: 30731163
- Jia Q et al. HNRNPA1-mediated 3' UTR length changes of HN1 contributes to cancer- and senescence-associated phenotypes. Aging (Albany NY) 11:4407-4437 (2019). PubMed: 31257225
- Williams AL et al. HIF1 mediates a switch in pyruvate kinase isoforms after myocardial infarction. Physiol Genomics 50:479-494 (2018). PubMed: 29652636
- Ke R et al. Prognostic value of heterogeneous ribonucleoprotein A1 expression and inflammatory indicators for patients with surgically resected hepatocellular carcinoma: Perspectives from a high occurrence area of hepatocellular carcinoma in China. Oncol Lett 16:3746-3756 (2018). PubMed: 30127985
- Leong SY et al. The role of Misshapen NCK-related kinase (MINK), a novel Ste20 family kinase, in the IRES-mediated protein translation of human enterovirus 71. PLoS Pathog 11:e1004686 (2015). PubMed: 25747578