Recombinant Anti-hnRNP A1 (citrulline R140) antibody [EPR20178] - BSA and Azide free (ab251488)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20178] to hnRNP A1 (citrulline R140) - BSA and Azide free
- Suitable for: Dot blot, WB
- Reacts with: Recombinant fragment
Related conjugates and formulations
Overview
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Product name
Anti-hnRNP A1 (citrulline R140) antibody [EPR20178] - BSA and Azide free
See all hnRNP A1 primary antibodies -
Description
Rabbit monoclonal [EPR20178] to hnRNP A1 (citrulline R140) - BSA and Azide free -
Host species
Rabbit -
Specificity
Based on sequence homology, we predict that this antibody may react with HNRNPA1L2.
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Tested applications
Suitable for: Dot blot, WBmore details -
Species reactivity
Reacts with: Recombinant fragment -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab251488 is the carrier-free version of ab208030.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Clonality
Monoclonal -
Clone number
EPR20178 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab251488 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Dot blot |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration. Detects a band of approximately 34 kDa (predicted molecular weight: 39 kDa).
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Notes |
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Dot blot
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Detects a band of approximately 34 kDa (predicted molecular weight: 39 kDa). |
Target
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Function
Involved in the packaging of pre-mRNA into hnRNP particles, transport of poly(A) mRNA from the nucleus to the cytoplasm and may modulate splice site selection. May play a role in HCV RNA replication. -
Sequence similarities
Contains 2 RRM (RNA recognition motif) domains. -
Post-translational
modificationsArg-194, Arg-206 and Arg-225 are dimethylated, probably to asymmetric dimethylarginine.
Sumoylated. -
Cellular localization
Nucleus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Shuttles continuously between the nucleus and the cytoplasm along with mRNA. Component of ribonucleosomes. In the course of viral infection, colocalizes with HCV NS5B at speckles in the cytoplasm in a HCV-replication dependent manner. - Information by UniProt
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Alternative names
- HNRNPA 1 antibody
- Helix destabilizing protein antibody
- Helix-destabilizing protein antibody
see all
Images
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This data was developed using ab208030, the same antibody clone in a different buffer formulation.Dot blot analysis of hnRNP A1 (citrulline R140) labeled with ab208030 at 1/1000 dilution. Lane 1: hnRNP A1 (citrulline R140) peptide. Lane 2: hnRNP A1 (citrulline R140) noncitrulline peptide.
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution was used as secondary antibody. Blocking and dilution buffer: 5% NFDM/TBST. Exposure time : 3 minutes. -
All lanes : Anti-hnRNP A1 (citrulline R140) antibody [EPR20178] (ab208030) at 1/1000 dilution
Lane 1 : Empty vector (vector control) transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : HEK-293T whole cell lysate transfected with GFP tagged PADI2 and treated with 10mM CaCl2 for 2h, then 10uM Ionomycin for 2h
Lane 3 : Empty vector (vector control) transfected HEK-293T whole cell lysate
Lane 4 : HEK-293T whole cell lysate transfected with GFP tagged PADI4 and treated with 10mM CaCl2 for 2h, then 10uM Ionomycin for 2h
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 39 kDa
Observed band size: 34 kDa why is the actual band size different from the predicted?
Exposure time: 5 secondsThis data was developed using ab208030, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab251488 has not yet been referenced specifically in any publications.