Recombinant
RabMAb

Recombinant Anti-hnRNP A1 (citrulline R92) antibody [EPR20174] (ab208026)

Overview

  • Product name

    Anti-hnRNP A1 (citrulline R92) antibody [EPR20174]
    See all hnRNP A1 primary antibodies
  • Description

    Rabbit monoclonal [EPR20174] to hnRNP A1 (citrulline R92)
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Dot blot, Flow Cyt, IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human hnRNP A1 (citrulline R92). The exact sequence is proprietary.
    Database link: P09651

  • Positive control

    • WB: HEK-293T transfected with GFP-tagged PADI2 and PADI4, respectively, treated with 10 mM calcium chloride and 10 µM Ionomycin, whole cell lysates. Flow Cyt: HEK-293T transfected with GFP-tagged PADI4, treated with 10 mM calcium chloride and 10 µM Ionomycin, cells. IP: HEK-293T transfected with GFP-tagged PADI4, treated with 10 mM calcium chloride and 10 µM Ionomycin, whole cell lysate.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 0.05% BSA, 40% Glycerol, PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR20174
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab208026 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Predicted molecular weight: 39 kDa.
Dot blot 1/1000.
Flow Cyt 1/600.
IP 1/30.

Target

  • Function

    Involved in the packaging of pre-mRNA into hnRNP particles, transport of poly(A) mRNA from the nucleus to the cytoplasm and may modulate splice site selection. May play a role in HCV RNA replication.
  • Sequence similarities

    Contains 2 RRM (RNA recognition motif) domains.
  • Post-translational
    modifications

    Arg-194, Arg-206 and Arg-225 are dimethylated, probably to asymmetric dimethylarginine.
    Sumoylated.
  • Cellular localization

    Nucleus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Shuttles continuously between the nucleus and the cytoplasm along with mRNA. Component of ribonucleosomes. In the course of viral infection, colocalizes with HCV NS5B at speckles in the cytoplasm in a HCV-replication dependent manner.
  • Information by UniProt
  • Database links

  • Alternative names

    • HNRNPA 1 antibody
    • Helix destabilizing protein antibody
    • Helix-destabilizing protein antibody
    • Heterogeneous nuclear ribonucleoprotein A1 antibody
    • Heterogeneous nuclear ribonucleoprotein A1B protein antibody
    • Heterogeneous nuclear ribonucleoprotein B2 protein antibody
    • Heterogeneous nuclear ribonucleoprotein core protein A1 antibody
    • hnRNP A1 antibody
    • hnRNP core protein A1 antibody
    • HNRNPA1 antibody
    • HNRPA1 antibody
    • MGC102835 antibody
    • Nuclear ribonucleoprotein particle A1 protein antibody
    • ROA1_HUMAN antibody
    • Single strand DNA binding protein UP1 antibody
    • Single strand RNA binding protein antibody
    • Single-strand RNA-binding protein antibody
    see all

Images

  • All lanes : Anti-hnRNP A1 (citrulline R92) antibody [EPR20174] (ab208026) at 1/2000 dilution

    Lanes 1 & 3 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with a control vector containing GFP tag, treated with 10 mM calcium chloride and 10 µM Ionomycin for 2 hours, whole cell lysate
    Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with GFP-tagged PADI2 (WT) expression vector, treated with 10 mM calcium chloride and 10 µM Ionomycin for 2 hours, whole cell lysate
    Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with GFP-tagged PADI4 (WT) expression vector, treated with 10 mM calcium chloride and 10 µM Ionomycin for 2 hours, whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 39 kDa


    Exposure time: 5 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    According to Uniprot annotation for HNRNPA1, isoform A1-A (34 kDa) is twenty times more abundant than isoform A1-B (39 kDa). The MW of HNRNPA1L2 is also 34 kD.

    In WB this antibody does not cross react with CCDC51 (expected MW 45 kDa).

  • Dot blot analysis of hnRNP A1 (citrulline R92) labeled with ab208026 at 1/1000 dilution.

    Lane 1: hnRNP A1 (citrulline R92) peptide.

    Lane 2: hnRNP A1 non-citrulline  peptide.

    Lane 3: hnRNP A3 (citrulline R113) peptide.

    Lane 4: hnRNP A0 (citrulline R85) peptide.

    Lane 5: CCDC51(citrulline R142) peptide.

    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution was used as secondary antibody.

    Based on sequence homology this antibody cross reacts with CCDC51 (citrulline R142) peptide (detected by dot blot only) and hnRNP A1L2 (citrulline R92) protein.

     

    Exposure time: 3 minutes.

    Blocking and dilution buffer: 5% NFDM/TBST.

     

  • Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell line transfected with GFP-tagged PADI4 (WT) expression vector, treated with 10 mM calcium chloride and 10 µM Ionomycin for 2 hours, labeling hnRNP A1 (citrulline R92) with ab208026 at 1/600 dilution (right panel) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (left panel). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) (150079) at 1/2000 dilution was used as the secondary antibody.

  • hnRNP A1 (citrulline R92) was immunoprecipitated from 0.35 mg of HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with GFP-tagged PADI4 (WT) expression vector, treated with 10 mM calcium chloride and 10 µM Ionomycin for 2 hours, whole cell lysate with ab208026 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab208026 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: HEK-293T transfected with GFP-tagged PADI4 (WT) expression vector, treated with 10 mM calcium chloride and 10 µM Ionomycin for 2 hours, whole cell lysate 10 µg (Input). 

    Lane 2: ab208026 IP in HEK-293T transfected with GFP-tagged PADI4 (WT) expression vector, treated with 10 mM calcium chloride and 10 µM Ionomycin for 2 hours, whole cell lysate. 

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab208026 in HEK-293T transfected with GFP-tagged PADI4 (WT) expression vector, treated with 10 mM calcium chloride and 10 µM Ionomycin for 2 hours, whole cell lysate.

     

    Exposure time: Less than 1 second.

    Blocking and dilution buffer: 5% NFDM/TBST.

     

References

ab208026 has not yet been referenced specifically in any publications.

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