Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20176] to hnRNP A1 (citrulline R97)
- Suitable for: WB, IP, Dot blot
- Reacts with: Human
Product nameAnti-hnRNP A1 (citrulline R97) antibody [EPR20176]
See all hnRNP A1 primary antibodies
DescriptionRabbit monoclonal [EPR20176] to hnRNP A1 (citrulline R97)
Based on sequence homology we predict that this antibody may react with hnRNP A1L2.
Tested applicationsSuitable for: WB, IP, Dot blotmore details
Species reactivityReacts with: Human
Synthetic peptide within Human hnRNP A1 aa 50-150 (citrulline R97). The exact sequence is proprietary.
Database link: P09651
- WB: HEK-293T transfected with PADI2 and PADI4, respectively, treated with CaCl2 and Ionomycin, whole cell lysates. IP: HEK-293T transfected with PADI4, treated with CaCl2 and Ionomycin, whole cell lysate.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab208028 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 38 kDa.|
FunctionInvolved in the packaging of pre-mRNA into hnRNP particles, transport of poly(A) mRNA from the nucleus to the cytoplasm and may modulate splice site selection. May play a role in HCV RNA replication.
Sequence similaritiesContains 2 RRM (RNA recognition motif) domains.
modificationsArg-194, Arg-206 and Arg-225 are dimethylated, probably to asymmetric dimethylarginine.
Cellular localizationNucleus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Shuttles continuously between the nucleus and the cytoplasm along with mRNA. Component of ribonucleosomes. In the course of viral infection, colocalizes with HCV NS5B at speckles in the cytoplasm in a HCV-replication dependent manner.
- Information by UniProt
- HNRNPA 1 antibody
- Helix destabilizing protein antibody
- Helix-destabilizing protein antibody
Lanes 1-2 & 4 : Anti-hnRNP A1 (citrulline R97) antibody [EPR20176] (ab208028) at 1/5000 dilution
Lane 3 : Anti-hnRNP A1 (citrulline R97) antibody [EPR20176] (ab208028) at 1/1000 dilution
Lanes 1 & 3 : Empty vector (vector control) transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen), whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate transfected with GFP-tagged PADI2 and treated with 10 mM CaCl2 for 2 hours, followed by 10 uM Ionomycin for 2 hours
Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney tranformed with large T antigen) whole cell lysate transfected with GFP- tagged PADI4 and treated with 10 mM CaCl2 for 2 hours, followed by 10 uM Ionomycin for 2 hours
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 38 kDa
Observed band size: 34 kDa why is the actual band size different from the predicted?
Exposure time: 4 seconds
Blocking/Dilution buffer: 5% NFDM/TBST.
According to Uniprot annotation, isoform A1-A (34kD) is twenty times more abundant than isoform A1-B (39kD). PADs (including PADI2 and PADI4) are the enzymes that hydrolyze arginine to form citrulline, and this catalytic reaction also needs Ca2+ and ionomycin. Please refer to PMID: 26360112.
Dot blot analysis of hnRNP A1 (citrulline R97) labeled with ab208028 at 1/1000 dilution.
Lane 1: hnRNP A1 (citrulline R97) peptide.
Lane 2:hnRNP A1 (citrulline R97) non-citrulline peptide.
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution was used as secondary antibody.
Blocking and dilution buffer: 5% NFDM/TBST.
hnRNP A1 (citrulline R97) was immunoprecipitated from 0.35 mg of HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with GFP tagged PADI4 and treated with 10 mM CaCl2 for 2 hours, then 10 µM Ionomycin for 2 hours whole cell lysate with ab208028 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab208028 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1: HEK-293T transfected with GFP tagged PADI4 and treated with 10 mM CaCl2 for 2 hours, then 10 µM Ionomycin for 2 hours whole cell lysate 10 µg (Input).
Lane 2: ab208028 IP in HEK-293T transfected with GFP tagged PADI4 and treated with 10 mM CaCl2 for 2 hours, then 10 µM Ionomycin for 2 hours whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab208028 in HEK-293T transfected with GFP tagged PADI4 and treated with 10 mM CaCl2 for 2 hours, then 10 µM Ionomycin for 2 hours whole cell lysate.
Exposure time: 10 seconds.
Blocking and dilution buffer: 5% NFDM/TBST.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab208028 has not yet been referenced specifically in any publications.