Overview

  • Product name

    Anti-hnRNP A1 (isoform A1-A) antibody
  • Description

    Rabbit polyclonal to hnRNP A1 (isoform A1-A)
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide:

    SSSQR

    , corresponding to a region around amino acids 190-194 of Human hnRNP A1 (isoform A1-A) (P09651).

  • Positive control

    • Recombinant Human hnRNP A1 (isoform A1-A) protein (ab91691) can be used as a positive control in WB. 293 cell extract

Properties

Applications

Our Abpromise guarantee covers the use of ab123378 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 34, 39 kDa (predicted molecular weight: 39 kDa). Incubate membrane with diluted antibody in 5% nonfat milk, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking over night.

Target

  • Function

    Involved in the packaging of pre-mRNA into hnRNP particles, transport of poly(A) mRNA from the nucleus to the cytoplasm and may modulate splice site selection. May play a role in HCV RNA replication.
  • Sequence similarities

    Contains 2 RRM (RNA recognition motif) domains.
  • Post-translational
    modifications

    Arg-194, Arg-206 and Arg-225 are dimethylated, probably to asymmetric dimethylarginine.
    Sumoylated.
  • Cellular localization

    Nucleus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Shuttles continuously between the nucleus and the cytoplasm along with mRNA. Component of ribonucleosomes. In the course of viral infection, colocalizes with HCV NS5B at speckles in the cytoplasm in a HCV-replication dependent manner.
  • Information by UniProt
  • Database links

  • Alternative names

    • Helix-destabilizing protein antibody
    • Heterogeneous nuclear ribonucleoprotein A1 antibody
    • HN RPA1L3 antibody
    • hnRNP A1 antibody
    • hnRNP core protein A1 antibody
    • HNRNPA1 antibody
    • M MGC102835 antibody
    • ROA1_HUMAN antibody
    • Single-strand RNA-binding protein antibody
    see all

Images

  • Anti-hnRNP A1 (isoform A1-A) antibody (ab123378) at 1/500 dilution + 293 cell extract

    Predicted band size: 39 kDa

References

This product has been referenced in:

  • Yuan T  et al. Low-density lipoprotein receptor-related protein 6 regulates alternative pre-mRNA splicing. J Cell Mol Med 22:4653-4663 (2018). Read more (PubMed: 30070011) »
See 1 Publication for this product

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