Anti-hnRNP A2B1 antibody (ab223237)
Key features and details
- Goat polyclonal to hnRNP A2B1
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-hnRNP A2B1 antibody
See all hnRNP A2B1 primary antibodies -
Description
Goat polyclonal to hnRNP A2B1 -
Host species
Goat -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Cow, Dog, Pig, Orangutan -
Immunogen
Synthetic peptide corresponding to Human hnRNP A2B1 aa 100-200 (internal sequence) (Cysteine residue). NP_002128.1; NP_112533.1.
Database link: P22626 -
Positive control
- WB: MCF7 cell lysate; NIH/3T3 nuclear lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab223237 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab223237 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).
1 hour primary incubation is recommended for this product. |
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IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa). 1 hour primary incubation is recommended for this product. |
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Involved with pre-mRNA processing. Forms complexes (ribonucleosomes) with at least 20 other different hnRNP and heterogeneous nuclear RNA in the nucleous. -
Sequence similarities
Contains 2 RRM (RNA recognition motif) domains. -
Cellular localization
Nucleus > nucleoplasm. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Component of ribonucleosomes. Predominantly nucleoplasmic, however isoform A2 is also found in the cytoplasm of cells in some tissues. Not found in the nucleolus. - Information by UniProt
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Database links
- Entrez Gene: 507564 Cow
- Entrez Gene: 3181 Human
- Entrez Gene: 100045191 Mouse
- Entrez Gene: 53379 Mouse
- Entrez Gene: 100174392 Orangutan
- Entrez Gene: 362361 Rat
- Omim: 600124 Human
- SwissProt: Q2HJ60 Cow
see all -
Alternative names
- Heterogeneous nuclear ribonucleoprotein A2 antibody
- Heterogeneous nuclear ribonucleoprotein A2/B1 antibody
- Heterogeneous nuclear ribonucleoprotein B1 antibody
see all
Images
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Lane 1 : Anti-hnRNP A2B1 antibody (ab223237) at 0.1 µg/ml
Lanes 2-3 : Anti-hnRNP A2B1 antibody (ab223237) at 0.01 µg/ml
Lane 1 : NIH3T3 cell lysate (in RIPA buffer)
Lane 2 : Nuclear NIH3T3 cell lysate (in RIPA buffer)
Lane 3 : NIH3T3 cell lysate (in RIPA buffer) with Peptide
Lysates/proteins at 35 µg per lane.
Predicted band size: 36 kDaDetected by chemiluminescence.
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Anti-hnRNP A2B1 antibody (ab223237) at 0.3 µg/ml + MCF7 (human breast adenocarcinoma cell line) cell lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 36 kDa
Observed band size: 36 kDaPrimary incubation was 1 hour.
Protocols
Datasheets and documents
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Datasheet download
References (0)
ab223237 has not yet been referenced specifically in any publications.