Key features and details
- Goat polyclonal to hnRNP A2B1
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-hnRNP A2B1 antibody
See all hnRNP A2B1 primary antibodies
DescriptionGoat polyclonal to hnRNP A2B1
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Cow, Dog, Pig, Orangutan
- WB: MCF7 cell lysate; NIH/3T3 nuclear lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab223237 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab223237 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).
1 hour primary incubation is recommended for this product.
|IHC-P||Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionInvolved with pre-mRNA processing. Forms complexes (ribonucleosomes) with at least 20 other different hnRNP and heterogeneous nuclear RNA in the nucleous.
Sequence similaritiesContains 2 RRM (RNA recognition motif) domains.
Cellular localizationNucleus > nucleoplasm. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Component of ribonucleosomes. Predominantly nucleoplasmic, however isoform A2 is also found in the cytoplasm of cells in some tissues. Not found in the nucleolus.
- Information by UniProt
- Heterogeneous nuclear ribonucleoprotein A2 antibody
- Heterogeneous nuclear ribonucleoprotein A2/B1 antibody
- Heterogeneous nuclear ribonucleoprotein B1 antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of human small intestine staining hnRNP with ab223237 at 5 μg/ml
Lane 1 : Anti-hnRNP A2B1 antibody (ab223237) at 0.1 µg/ml
Lanes 2-3 : Anti-hnRNP A2B1 antibody (ab223237) at 0.01 µg/ml
Lane 1 : NIH3T3 cell lysate (in RIPA buffer)
Lane 2 : Nuclear NIH3T3 cell lysate (in RIPA buffer)
Lane 3 : NIH3T3 cell lysate (in RIPA buffer) with Peptide
Lysates/proteins at 35 µg per lane.
Predicted band size: 36 kDa
Detected by chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of human testies staining hnRNP with ab223237 at 5 μg/ml
Anti-hnRNP A2B1 antibody (ab223237) at 0.3 µg/ml + MCF7 (human breast adenocarcinoma cell line) cell lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 36 kDa
Observed band size: 36 kDa
Primary incubation was 1 hour.
ab223237 has not yet been referenced specifically in any publications.