Overview

  • Product name

  • Description

    Goat polyclonal to hnRNP A2B1
  • Host species

    Goat
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow, Dog, Pig, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human hnRNP A2B1 aa 139-149 (internal sequence) (Cysteine residue). NP_002128.1; NP_112533.1.
    Sequence:

    C-DTIEIITDRQS


    Database link: P22626

  • Positive control

    • WB: MCF7 cell lysate; NIH/3T3 nuclear lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 0.5% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab223237 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab223237 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Involved with pre-mRNA processing. Forms complexes (ribonucleosomes) with at least 20 other different hnRNP and heterogeneous nuclear RNA in the nucleous.
  • Sequence similarities

    Contains 2 RRM (RNA recognition motif) domains.
  • Cellular localization

    Nucleus > nucleoplasm. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Component of ribonucleosomes. Predominantly nucleoplasmic, however isoform A2 is also found in the cytoplasm of cells in some tissues. Not found in the nucleolus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Heterogeneous nuclear ribonucleoprotein A2 antibody
    • Heterogeneous nuclear ribonucleoprotein A2/B1 antibody
    • Heterogeneous nuclear ribonucleoprotein B1 antibody
    • Heterogeneous nuclear ribonucleoproteins A2/B1 antibody
    • hnRNP A2 / hnRNP B1 antibody
    • hnRNP A2 antibody
    • hnRNP A2/B1 antibody
    • hnRNP B1 antibody
    • hnRNP-A2 antibody
    • hnRNP-B1 antibody
    • hnRNPA2 antibody
    • Hnrnpa2b1 antibody
    • hnRNPB1 antibody
    • HNRPA2 antibody
    • HNRPA2B1 antibody
    • HNRPB1 antibody
    • Nuclear ribonucleoprotein particle A2 protein antibody
    • RNP A2 antibody
    • RNP B1 antibody
    • RNP-A2 antibody
    • RNP-B1 antibody
    • RNPA2 antibody
    • RNPB1 antibody
    • ROA2_HUMAN antibody
    • SNRPB1 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of human small intestine staining hnRNP with ab223237 at 5 μg/ml

  • Lane 1 : Anti-hnRNP A2B1 antibody (ab223237) at 0.1 µg/ml
    Lanes 2-3 : Anti-hnRNP A2B1 antibody (ab223237) at 0.01 µg/ml

    Lane 1 : NIH3T3 cell lysate (in RIPA buffer)
    Lane 2 : Nuclear NIH3T3 cell lysate (in RIPA buffer)
    Lane 3 : NIH3T3 cell lysate (in RIPA buffer) with Peptide

    Lysates/proteins at 35 µg per lane.

    Predicted band size: 36 kDa



    Detected by chemiluminescence.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of human testies staining hnRNP with ab223237 at 5 μg/ml

  • Anti-hnRNP A2B1 antibody (ab223237) at 0.3 µg/ml + MCF7 (human breast adenocarcinoma cell line) cell lysate (in RIPA buffer) at 35 µg

    Developed using the ECL technique.

    Predicted band size: 36 kDa
    Observed band size: 36 kDa



    Primary incubation was 1 hour.

References

ab223237 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab223237.
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