Overview

  • Product name

  • Description

    Rabbit polyclonal to hnRNP A2B1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Cow, Rhesus monkey
  • Immunogen

    Recombinant fragment within Human hnRNP A2B1 (internal sequence). The exact sequence is proprietary.
    Database link: P22626

  • Positive control

    • WB: HEK-293T whole cell extract; A431 whole cell lysate. ICC/IF: HeLa cells. IHC-P: Human breast cancer tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab227465 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/20000. Predicted molecular weight: 37 kDa.
IHC-P 1/100 - 1/1000.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Involved with pre-mRNA processing. Forms complexes (ribonucleosomes) with at least 20 other different hnRNP and heterogeneous nuclear RNA in the nucleous.
  • Sequence similarities

    Contains 2 RRM (RNA recognition motif) domains.
  • Cellular localization

    Nucleus > nucleoplasm. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Component of ribonucleosomes. Predominantly nucleoplasmic, however isoform A2 is also found in the cytoplasm of cells in some tissues. Not found in the nucleolus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Heterogeneous nuclear ribonucleoprotein A2 antibody
    • Heterogeneous nuclear ribonucleoprotein A2/B1 antibody
    • Heterogeneous nuclear ribonucleoprotein B1 antibody
    • Heterogeneous nuclear ribonucleoproteins A2/B1 antibody
    • hnRNP A2 / hnRNP B1 antibody
    • hnRNP A2 antibody
    • hnRNP A2/B1 antibody
    • hnRNP B1 antibody
    • hnRNP-A2 antibody
    • hnRNP-B1 antibody
    • hnRNPA2 antibody
    • Hnrnpa2b1 antibody
    • hnRNPB1 antibody
    • HNRPA2 antibody
    • HNRPA2B1 antibody
    • HNRPB1 antibody
    • Nuclear ribonucleoprotein particle A2 protein antibody
    • RNP A2 antibody
    • RNP B1 antibody
    • RNP-A2 antibody
    • RNP-B1 antibody
    • RNPA2 antibody
    • RNPB1 antibody
    • ROA2_HUMAN antibody
    • SNRPB1 antibody
    see all

Images

  • All lanes : Anti-hnRNP A2B1 antibody (ab227465) at 1/5000 dilution

    Lane 1 : Non-transfected A431 (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extracts
    Lane 2 : hnRNP A2B1-transfected A431 (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extracts

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 37 kDa



    12% SDS-PAGE gel.

  • Paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for hnRNP A2B1 (green) using ab227465 at 1/500 dilution in ICC/IF.

    Counterstain: alpha tubulin filaments (red).

  • Paraffin-embedded human breast cancer tissue stained for hnRNP A2B1 using ab227465 at 1/250 dilution in immunohistochemical analysis.

  • Anti-hnRNP A2B1 antibody (ab227465) at 1/10000 dilution + A431 (human epidermoid carcinoma cell line) whole cell lysate at 30 µg

    Predicted band size: 37 kDa



    12% SDS-PAGE gel.

References

ab227465 has not yet been referenced specifically in any publications.

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