Product nameAnti-hnRNP A2B1 antibody [DP3B3]
See all hnRNP A2B1 primary antibodies
DescriptionMouse monoclonal [DP3B3] to hnRNP A2B1
Tested applicationsSuitable for: ICC/IF, IHC-Fr, ELISA, WB, IP, IHC-Pmore details
Species reactivityReacts with: Chicken, Human, Pig
Bacterially expressed His-hnRNPA2 fusion protein (Human).
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.1% Sodium azide
Concentration information loading...
PurityProtein A purified
Purification notesPurified from supernatant.
Our Abpromise guarantee covers the use of ab6102 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|IHC-Fr||1/100. PubMed: 17067748Fix with acetone.|
|ELISA||Use at an assay dependent concentration.|
|WB||1/1500 - 1/6000. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa).|
|IP||Use at an assay dependent concentration.|
|IHC-P||Use a concentration of 1 µg/ml.|
FunctionInvolved with pre-mRNA processing. Forms complexes (ribonucleosomes) with at least 20 other different hnRNP and heterogeneous nuclear RNA in the nucleous.
Sequence similaritiesContains 2 RRM (RNA recognition motif) domains.
Cellular localizationNucleus > nucleoplasm. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Component of ribonucleosomes. Predominantly nucleoplasmic, however isoform A2 is also found in the cytoplasm of cells in some tissues. Not found in the nucleolus.
- Information by UniProt
- Heterogeneous nuclear ribonucleoprotein A2 antibody
- Heterogeneous nuclear ribonucleoprotein A2/B1 antibody
- Heterogeneous nuclear ribonucleoprotein B1 antibody
All lanes : Anti-hnRNP A2B1 antibody [DP3B3] (ab6102) at 1/1000 dilution
Lane 1 : HeLa (hnRNPA2 shRNA-1) at 1/1000 dilution
Lane 2 : HeLa (hnRNPA2 shRNA-1)
Lane 3 : HeLa hnRNPA2 shRNA-2 at 1/1000 dilution
Lane 4 : HeLa hnRNPA2 shRNA-2
Lanes 5-6 : HeLa (Control shRNA)
Lane 7 : Untreated HeLa
All lanes : HRP-conjugated Goat anti-mouse polyclonal at 1/1000 dilution
Predicted band size: 37 kDa
ab6102 staining hnRNP A2B1 in human A549 cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed with formaldehyde, permeabilized with Triton X-100 and blocking with 10% serum at 200C for 1 hour was performed. Samples were incubated with primary antibody (1/100: in PBS containing 10% FCS) for 1 hour at 200C. An Alexa Fluor ® 488-conjugated goat polyclonal to mouse IgG was used at dilution at 1/300 as secondary antibody.
ab6102 (1µg/ml) staining hnRNP A2B1 in human liver.
Sections were stained using an automated system (DAKO Autostainer Plus), at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate pH6.1 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
ab6102 (1µg/ml) staining hnRNP A2B1 in human caecum, using an automated system (DAKO Autostainer Plus). Using this protocol there is strong nuclear staining in the mucosal epithelium.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
This product has been referenced in:
- Majewski L et al. Myosin VI in the nucleus of neurosecretory PC12 cells: Stimulation-dependent nuclear translocation and interaction with nuclear proteins. Nucleus 9:125-141 (2018). Read more (PubMed: 29293066) »
- Kuljittichanok D et al. Effect of Derris scandens extract on a human hepatocellular carcinoma cell line. Oncol Lett 16:1943-1952 (2018). Read more (PubMed: 30034552) »