Overview

  • Product name
    Anti-hnRNP A2B1 antibody [DP3B3]
    See all hnRNP A2B1 primary antibodies
  • Description
    Mouse monoclonal [DP3B3] to hnRNP A2B1
  • Host species
    Mouse
  • Tested applications
    Suitable for: ICC/IF, IHC-Fr, ELISA, WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Chicken, Human, Pig
  • Immunogen

    Bacterially expressed His-hnRNPA2 fusion protein (Human).

Properties

Applications

Our Abpromise guarantee covers the use of ab6102 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
IHC-Fr 1/100. PubMed: 17067748Fix with acetone.
ELISA Use at an assay dependent concentration.
WB 1/1500 - 1/6000. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa).
IP Use at an assay dependent concentration.
IHC-P Use a concentration of 1 µg/ml.

Target

  • Function
    Involved with pre-mRNA processing. Forms complexes (ribonucleosomes) with at least 20 other different hnRNP and heterogeneous nuclear RNA in the nucleous.
  • Sequence similarities
    Contains 2 RRM (RNA recognition motif) domains.
  • Cellular localization
    Nucleus > nucleoplasm. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Component of ribonucleosomes. Predominantly nucleoplasmic, however isoform A2 is also found in the cytoplasm of cells in some tissues. Not found in the nucleolus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Heterogeneous nuclear ribonucleoprotein A2 antibody
    • Heterogeneous nuclear ribonucleoprotein A2/B1 antibody
    • Heterogeneous nuclear ribonucleoprotein B1 antibody
    • Heterogeneous nuclear ribonucleoproteins A2/B1 antibody
    • hnRNP A2 / hnRNP B1 antibody
    • hnRNP A2 antibody
    • hnRNP A2/B1 antibody
    • hnRNP B1 antibody
    • hnRNP-A2 antibody
    • hnRNP-B1 antibody
    • hnRNPA2 antibody
    • Hnrnpa2b1 antibody
    • hnRNPB1 antibody
    • HNRPA2 antibody
    • HNRPA2B1 antibody
    • HNRPB1 antibody
    • Nuclear ribonucleoprotein particle A2 protein antibody
    • RNP A2 antibody
    • RNP B1 antibody
    • RNP-A2 antibody
    • RNP-B1 antibody
    • RNPA2 antibody
    • RNPB1 antibody
    • ROA2_HUMAN antibody
    • SNRPB1 antibody
    see all

Images

  • All lanes : Anti-hnRNP A2B1 antibody [DP3B3] (ab6102) at 1/1000 dilution

    Lane 1 : HeLa (hnRNPA2 shRNA-1) at 1/1000 dilution
    Lane 2 : HeLa (hnRNPA2 shRNA-1)
    Lane 3 : HeLa hnRNPA2 shRNA-2 at 1/1000 dilution
    Lane 4 : HeLa hnRNPA2 shRNA-2
    Lanes 5-6 : HeLa (Control shRNA)
    Lane 7 : Untreated HeLa

    Secondary
    All lanes : HRP-conjugated Goat anti-mouse polyclonal at 1/1000 dilution

    Predicted band size: 37 kDa

    See Abreview

  • ab6102 staining hnRNP A2B1 in human A549 cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed with formaldehyde, permeabilized with Triton X-100 and blocking with 10% serum at 200C for 1 hour was performed. Samples were incubated with primary antibody (1/100: in PBS containing 10% FCS) for 1 hour at 200C. An Alexa Fluor ® 488-conjugated goat polyclonal to mouse IgG was used at dilution at 1/300 as secondary antibody.

    See Abreview

  • ab6102 (1µg/ml) staining hnRNP A2B1 in human liver.
    Sections were stained using an automated system (DAKO Autostainer Plus), at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate pH6.1 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
  • ab6102 (1µg/ml) staining hnRNP A2B1 in human caecum, using an automated system (DAKO Autostainer Plus). Using this protocol there is strong nuclear staining in the mucosal epithelium.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

References

This product has been referenced in:
  • Majewski L  et al. Myosin VI in the nucleus of neurosecretory PC12 cells: Stimulation-dependent nuclear translocation and interaction with nuclear proteins. Nucleus 9:125-141 (2018). Read more (PubMed: 29293066) »
  • Kuljittichanok D  et al. Effect of Derris scandens extract on a human hepatocellular carcinoma cell line. Oncol Lett 16:1943-1952 (2018). Read more (PubMed: 30034552) »
See all 28 Publications for this product

Customer reviews and Q&As

1-10 of 12 Abreviews or Q&A

Answer

Thank you for your enquiry regarding ab6102 and for taking the time to provide some useful details of the experiments. I am very sorry to hear that you are having problems with this antibody. Band at 37 kDa in input sample indicate that the antibody is working fine and further optimizations in antibody dilutions could help getting clean band at right size. Based on the information provided I presume that antibody is unable to IP the protein. So in order to understand the problem I would like to ask few questions; - What type of beads were used for IP? e.g. protein A or G - How the IP was performed? Have you mixed antibody beads and lysates once, or beads and antibody first then lysates or antibody lysates and then beads? -  For how long the mixture was incubated? - Can we define the problem as no bands in IP or multiple bands; because loading 1000 ug protein per well in WB is way too high. If it is not true then I am presuming that this is the amount of protein (lysates) you have used to mix with antibody and beads. - 1/1000 dilution means the antibody was used at 1ug/ml which is very low; I would recommend using ab at 5 ug/ml and 10ug/ml. - Could you send us an image? I hope the suggestion of conc. will help to improve the results. I will also look forward receiving reply to my questions soon.

Read More
Application
Immunocytochemistry
Sample
Mouse Cell (Retina)
Permeabilization
Yes - freeze/thaw
Specification
Retina
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Oct 27 2015

Answer

AB18293; Rabbit anti- NRA52/LRH-1 Lot GR9515-6 = 0.290 mg/ml;  Lot: GR34910-1 = 0.220 mg/ml AB10685; Mouse anti- hnRNPA1 [4B10] Lot: GR14303-5 =  1 mg/ml AB6102; Mouse anti- hnRNPA2/B1 [DP3B3] Lot: GR21753-3 = 1 mg/ml AB16667 Rabbit anti- Ki67  Lot: GR40326-2 & Lot:GR17359-1. This product is supplied as a tissue culture supernatent. As there will be other proteins within this supernatent, specific antibody concentration can not be measured. Typically the concentration of tissue culture supernatants is between 10-50 ug/ml.

Read More

Question
Answer

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number 979027 with ab31645. To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I wish you the best of luck with your research.  

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Answer

For dispatching the requested product, we would need the order number of original purchase. Could you please send this asap? Looking forward to hearing from you soon.  

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Answer

Thank you very much for sending the image and all the details. I acknowledge the time you have spent trying this antibody and convinced that the observed band size is not at 37 kDa. The antibody should be detecting the band at right size; the rat protein is approx 99% similar to human protein. I unfortunately can‘t compare the results with our own lab data because ab6102 was never tested with rat lysates. It may be worth going through the literature and comparing the results with other scientist’s findings. In order to proceed further in this case I can either send you a free of charge replacement of ab6102 from different lot or can also dispatch a different antibody e.g. ab31645 or ab24409 or ab64800; although these antibodies are not tested in IP however I don’t see a reason why these antibodies can’t be used in IP. If you are interested in trying these let me know I will then send you a vial of ab.  I also want to point out the background in IgG IP which kind of show similar pattern as IP of A2; I am not sure why this is, the IgG IP should be clean; one reason could be the high protein conc. as compare to the antibody. I will look forward to receiving your reply soon. Please do not hesitate to contact me if you have any question.  

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Human Bladder Cancer)
Specification
Human Bladder Cancer
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated
Permeabilization
No

Abcam user community

Verified customer

Submitted May 11 2011

Application
Western blot
Sample
Mouse Cell lysate - whole cell (NSC34)
Loading amount
10 µg
Specification
NSC34
Gel Running Conditions
Reduced Denaturing (4-12% nupage gel)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Nov 12 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa)
Loading amount
30 µg
Specification
HeLa
Gel Running Conditions
Reduced Denaturing (12.5%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Nov 26 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (A549)
Specification
A549
Fixative
Formaldehyde
Permeabilization
Yes - tx 100
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Jun 05 2009

1-10 of 12 Abreviews or Q&A

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