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    hnrnp-a2b1-antibody-dp3b3-ab6102.pdf

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Epigenetics and Nuclear Signaling DNA / RNA RNA Processing
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Anti-hnRNP A2B1 antibody [DP3B3] (ab6102)

  • Datasheet
  • SDS
Reviews (7)Q&A (5)References (39)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP A2B1 antibody [DP3B3] (ab6102)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP A2B1 antibody [DP3B3] (ab6102)

Key features and details

  • Mouse monoclonal [DP3B3] to hnRNP A2B1
  • Suitable for: IHC-P
  • Reacts with: Human
  • Isotype: IgG2a

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Overview

  • Product name

    Anti-hnRNP A2B1 antibody [DP3B3]
    See all hnRNP A2B1 primary antibodies
  • Description

    Mouse monoclonal [DP3B3] to hnRNP A2B1
  • Host species

    Mouse
  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    See all applications and species data
  • Immunogen

    Bacterially expressed His-hnRNPA2 fusion protein (Human).

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.1% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Purification notes

    Purified from supernatant.
  • Clonality

    Monoclonal
  • Clone number

    DP3B3
  • Myeloma

    Sp2/0
  • Isotype

    IgG2a
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • RNA Processing
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • RNA Processing
    • Other

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG2a, kappa monoclonal [MG2a-53] - Isotype control (ab18415)
  • Recombinant Protein

    • Recombinant Human hnRNP A2B1 protein (ab224866)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab6102 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IHC-P
Human
Application Abreviews Notes
IHC-P (1)
Use a concentration of 1 µg/ml.
Notes
IHC-P
Use a concentration of 1 µg/ml.

Target

  • Function

    Involved with pre-mRNA processing. Forms complexes (ribonucleosomes) with at least 20 other different hnRNP and heterogeneous nuclear RNA in the nucleous.
  • Sequence similarities

    Contains 2 RRM (RNA recognition motif) domains.
  • Cellular localization

    Nucleus > nucleoplasm. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Component of ribonucleosomes. Predominantly nucleoplasmic, however isoform A2 is also found in the cytoplasm of cells in some tissues. Not found in the nucleolus.
  • Target information above from: UniProt accession P22626 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 3181 Human
    • Omim: 600124 Human
    • SwissProt: P22626 Human
    • Unigene: 487774 Human
    • Alternative names

      • Heterogeneous nuclear ribonucleoprotein A2 antibody
      • Heterogeneous nuclear ribonucleoprotein A2/B1 antibody
      • Heterogeneous nuclear ribonucleoprotein B1 antibody
      • Heterogeneous nuclear ribonucleoproteins A2/B1 antibody
      • hnRNP A2 / hnRNP B1 antibody
      • hnRNP A2 antibody
      • hnRNP A2/B1 antibody
      • hnRNP B1 antibody
      • hnRNP-A2 antibody
      • hnRNP-B1 antibody
      • hnRNPA2 antibody
      • Hnrnpa2b1 antibody
      • hnRNPB1 antibody
      • HNRPA2 antibody
      • HNRPA2B1 antibody
      • HNRPB1 antibody
      • Nuclear ribonucleoprotein particle A2 protein antibody
      • RNP A2 antibody
      • RNP B1 antibody
      • RNP-A2 antibody
      • RNP-B1 antibody
      • RNPA2 antibody
      • RNPB1 antibody
      • ROA2_HUMAN antibody
      • SNRPB1 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP A2B1 antibody [DP3B3] (ab6102)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP A2B1 antibody [DP3B3] (ab6102)
      ab6102 (1µg/ml) staining hnRNP A2B1 in human liver.
      Sections were stained using an automated system (DAKO Autostainer Plus), at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate pH6.1 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP A2B1 antibody [DP3B3] (ab6102)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP A2B1 antibody [DP3B3] (ab6102)
      ab6102 (1µg/ml) staining hnRNP A2B1 in human caecum, using an automated system (DAKO Autostainer Plus). Using this protocol there is strong nuclear staining in the mucosal epithelium.
      Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

    Protocols

    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (39)

    Publishing research using ab6102? Please let us know so that we can cite the reference in this datasheet.

    ab6102 has been referenced in 39 publications.

    • Zhang Y  et al. Long non-coding RNA H19 promotes colorectal cancer metastasis via binding to hnRNPA2B1. J Exp Clin Cancer Res 39:141 (2020). PubMed: 32698890
    • Coppola A  et al. Anti-Inflammatory Action of Heterogeneous Nuclear Ribonucleoprotein A2/B1 in Patients with Autoimmune Endocrine Disorders. J Clin Med 9:N/A (2019). PubMed: 31861546
    • Kwon J  et al. Functional roles of hnRNPA2/B1 regulated by METTL3 in mammalian embryonic development. Sci Rep 9:8640 (2019). PubMed: 31201338
    • Val S  et al. Nontypeable Haemophilus influenzae lysates increase heterogeneous nuclear ribonucleoprotein secretion and exosome release in human middle-ear epithelial cells. FASEB J 32:1855-1867 (2018). PubMed: 29191962
    • Morita M  et al. PKM1 Confers Metabolic Advantages and Promotes Cell-Autonomous Tumor Cell Growth. Cancer Cell 33:355-367.e7 (2018). PubMed: 29533781
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-10 of 12 Abreviews or Q&A

    Question

    LOT NUMBER GR21753-2 ORDER NUMBER -- NOT SPECIFIED -- DESCRIPTION OF THE PROBLEM Non-specific band WB from IP samples show no band for 37kDa zone also, the input lane shows two bands (strong one at 50kDa and a weak band at 37kDa) SAMPLE Rat primary hippocampal cell culture PRIMARY ANTIBODY 1:1000 diluted in 0,5% milk TBS-T Washed 3x 10min with TBS-T DETECTION METHOD ECF, 5min max ANTIBODY STORAGE CONDITIONS 4ºC (short-term) -20ºC (aliquots) SAMPLE PREPARATION RIPA supplemented with proteases inhibitors (CLAP and PMSF), phoshpatase inhibitor (NaF), DTT and RNase inhibitor Samples from IP were dissolved with sample buffer 2x and heated at 95ºC for 5min AMOUNT OF PROTEIN LOADED 50ug for Input 1000ug for IP ELECTROPHORESIS/GEL CONDITIONS SDS-PAGE, tris-bicine, 12,5% polyacrylamide TRANSFER AND BLOCKING CONDITIONS CAPS-methanol, 40V o.n. 4ºC blocked with 5% milk TBS-Tween SECONDARY ANTIBODY Anti-Mouse 1:20000 diluted in 0,5% milk TBS-T Washed 3x 10min with TBS-T HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 2 HAVE YOU RUN A "NO PRIMARY" CONTROL? No DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes WHAT STEPS HAVE YOU ALTERED? Adding the flow-through samples to the WB

    Read More

    Abcam community

    Verified customer

    Asked on Oct 28 2011

    Answer

    Thank you for your enquiry regarding ab6102 and for taking the time to provide some useful details of the experiments. I am very sorry to hear that you are having problems with this antibody. Band at 37 kDa in input sample indicate that the antibody is working fine and further optimizations in antibody dilutions could help getting clean band at right size. Based on the information provided I presume that antibody is unable to IP the protein. So in order to understand the problem I would like to ask few questions; - What type of beads were used for IP? e.g. protein A or G - How the IP was performed? Have you mixed antibody beads and lysates once, or beads and antibody first then lysates or antibody lysates and then beads? -  For how long the mixture was incubated? - Can we define the problem as no bands in IP or multiple bands; because loading 1000 ug protein per well in WB is way too high. If it is not true then I am presuming that this is the amount of protein (lysates) you have used to mix with antibody and beads. - 1/1000 dilution means the antibody was used at 1ug/ml which is very low; I would recommend using ab at 5 ug/ml and 10ug/ml. - Could you send us an image? I hope the suggestion of conc. will help to improve the results. I will also look forward receiving reply to my questions soon.

    Read More

    Abcam Scientific Support

    Answered on Oct 28 2011

    Immunocytochemistry abreview for Anti-hnRNP A2B1 antibody [DP3B3]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry
    Sample
    Mouse Cell (Retina)
    Permeabilization
    Yes - freeze/thaw
    Specification
    Retina
    Blocking step
    BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Oct 27 2015

    Question

    we currently using mouse IgG and Ran IgG for -ve control, however, the -ve staining comes faster and stronger than +ve control, we already dilute the IgG same concentration as those antibodies. but it still comes stronger. I would like to know the actual concentration for those antibodies to see whether there is an concentration unequally cause the strong -ve staining.

    Read More

    Abcam community

    Verified customer

    Asked on Dec 20 2012

    Answer

    AB18293; Rabbit anti- NRA52/LRH-1 Lot GR9515-6 = 0.290 mg/ml;  Lot: GR34910-1 = 0.220 mg/ml AB10685; Mouse anti- hnRNPA1 [4B10] Lot: GR14303-5 =  1 mg/ml AB6102; Mouse anti- hnRNPA2/B1 [DP3B3] Lot: GR21753-3 = 1 mg/ml AB16667 Rabbit anti- Ki67  Lot: GR40326-2 & Lot:GR17359-1. This product is supplied as a tissue culture supernatent. As there will be other proteins within this supernatent, specific antibody concentration can not be measured. Typically the concentration of tissue culture supernatants is between 10-50 ug/ml.

    Read More

    Abcam Scientific Support

    Answered on Dec 20 2012

    Question

    The order number is 15032.1.32-ET-MG.

    Read More

    Abcam community

    Verified customer

    Asked on Nov 04 2011

    Answer

    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number 979027 with ab31645. To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I wish you the best of luck with your research.  

    Read More

    Abcam Scientific Support

    Answered on Nov 04 2011

    Question

    I would prefer a different antibody than a vial from the same (since it is monoclonal, i'm not sure if the results would improve that much). Since the Anti-hnRNP A2B1 antibody (ab31645) works for ICC/IF it might work either with IP experiments. Being so, i would be appreciated if you could dispatch the one that i've refered. Thank you for your support,

    Read More

    Abcam community

    Verified customer

    Asked on Nov 02 2011

    Answer

    For dispatching the requested product, we would need the order number of original purchase. Could you please send this asap? Looking forward to hearing from you soon.  

    Read More

    Abcam Scientific Support

    Answered on Nov 02 2011

    Question

    What type of beads were used for IP? *Protein G-agarose beads* > How the IP was performed? Have you mixed antibody beads and lysates once, or beads and antibody first then lysates or antibody lysates and then beads? *Antibody (6ug) with 100ul of beads o.n., 4ºc in the next day, 1mg of protein was incubated during 1h at 4ºC* >For how long the mixture was incubated? *1h* >Can we define the problem as no bands in IP or multiple bands because loading 1000 ug protein per well in WB is way too high. If it is not true then I am presuming that this is the amount of protein (lysates) you have used to mix with antibody and beads. *1mg was used to the IP for the input, 50ug of protein* > 1/1000 dilution means the antibody was used at 1ug/ml which is very low I would recommend using ab at 5 ug/ml and 10ug/ml. *1/1000 was the dilution used in the 3 abreviews that were availabe together with the datasheet. So the dilution is not the problem at least in the input lane it was supposed to see a stronger band at 37kDa and none at 50kDa.* > Could you send us an image? *Sorry, i attached a file previously, but then the page have reloaded because i've spent more than 20min filling the form. How can i send you the image? I've tried to reply by e-mail but it was not delivered... Here's a link with the image that i wanted to attach: http://imageshack.us/f/31/hnrnpa2.png/

    Read More

    Abcam community

    Verified customer

    Asked on Oct 28 2011

    Answer

    Thank you very much for sending the image and all the details. I acknowledge the time you have spent trying this antibody and convinced that the observed band size is not at 37 kDa. The antibody should be detecting the band at right size; the rat protein is approx 99% similar to human protein. I unfortunately can‘t compare the results with our own lab data because ab6102 was never tested with rat lysates. It may be worth going through the literature and comparing the results with other scientist’s findings. In order to proceed further in this case I can either send you a free of charge replacement of ab6102 from different lot or can also dispatch a different antibody e.g. ab31645 or ab24409 or ab64800; although these antibodies are not tested in IP however I don’t see a reason why these antibodies can’t be used in IP. If you are interested in trying these let me know I will then send you a vial of ab.  I also want to point out the background in IgG IP which kind of show similar pattern as IP of A2; I am not sure why this is, the IgG IP should be clean; one reason could be the high protein conc. as compare to the antibody. I will look forward to receiving your reply soon. Please do not hesitate to contact me if you have any question.  

    Read More

    Abcam Scientific Support

    Answered on Oct 28 2011

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-hnRNP A2B1 antibody [DP3B3]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Human Bladder Cancer)
    Specification
    Human Bladder Cancer
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated
    Permeabilization
    No
    Read More

    Abcam user community

    Verified customer

    Submitted May 11 2011

    Western blot abreview for Anti-hnRNP A2B1 antibody [DP3B3]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (NSC34)
    Loading amount
    10 µg
    Specification
    NSC34
    Gel Running Conditions
    Reduced Denaturing (4-12% nupage gel)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Nov 12 2010

    Western blot abreview for Anti-hnRNP A2B1 antibody [DP3B3]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HeLa)
    Loading amount
    30 µg
    Specification
    HeLa
    Gel Running Conditions
    Reduced Denaturing (12.5%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Nov 26 2009

    Immunocytochemistry/ Immunofluorescence abreview for Anti-hnRNP A2B1 antibody [DP3B3]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (A549)
    Specification
    A549
    Fixative
    Formaldehyde
    Permeabilization
    Yes - tx 100
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
    Read More

    Abcam user community

    Verified customer

    Submitted Jun 05 2009

    1-10 of 12 Abreviews or Q&A

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