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we currently using mouse IgG and Ran IgG for -ve control, however, the -ve staining comes faster and stronger than +ve control, we already dilute the IgG same concentration as those antibodies. but it still comes stronger. I would like to know the actual concentration for those antibodies to see whether there is an concentration unequally cause the strong -ve staining.
Asked on Dec 20 2012
AB18293; Rabbit anti- NRA52/LRH-1 Lot GR9515-6 = 0.290 mg/ml; Lot: GR34910-1 = 0.220 mg/ml AB10685; Mouse anti- hnRNPA1 [4B10] Lot: GR14303-5 = 1 mg/ml AB6102; Mouse anti- hnRNPA2/B1 [DP3B3] Lot: GR21753-3 = 1 mg/ml AB16667 Rabbit anti- Ki67 Lot: GR40326-2 & Lot:GR17359-1. This product is supplied as a tissue culture supernatent. As there will be other proteins within this supernatent, specific antibody concentration can not be measured. Typically the concentration of tissue culture supernatants is between 10-50 ug/ml.
Answered on Dec 20 2012