Overview

  • Product name

    Anti-hnRNP C1 + C2/HNRNPC antibody [55.1_3A9]
    See all hnRNP C1 + C2/HNRNPC primary antibodies
  • Description

    Mouse monoclonal [55.1_3A9] to hnRNP C1 + C2/HNRNPC
  • Host species

    Mouse
  • Specificity

    ab128049 specificity has been determined by protein microarray.
  • Tested applications

    Suitable for: WB, IP, ChIP, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Tissue, cells or virus corresponding to Human hnRNP C1 + C2/HNRNPC.

  • Positive control

    • HeLa cells.
  • General notes

     This product was previously labelled as hnRNP C1 + C2

     

Properties

Applications

Our Abpromise guarantee covers the use of ab128049 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 33 kDa.
IP Use a concentration of 4 µg/ml.
ChIP Use at an assay dependent dilution.
ICC/IF 1/100. 4% paraformaldehyde fixed, 0.1% Triton X-100 permeabilized

Target

  • Function

    Binds pre-mRNA and nucleates the assembly of 40S hnRNP particles (PubMed:8264621). Specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs, a modification present at internal sites of mRNAs that affects mRNA splicing, processing and stability. M6A alters the local structure in mRNAs and long non-coding RNAs (lncRNAs) via a mechanism named 'm(6)A-switch' to facilitate binding of HNRNPC, leading to regulation of mRNA splicing (PubMed:25719671). Single HNRNPC tetramers bind 230-240 nucleotides. Trimers of HNRNPC tetramers bind 700 nucleotides. May play a role in the early steps of spliceosome assembly and pre-mRNA splicing. Interacts with poly-U tracts in the 3'-UTR or 5'-UTR of mRNA and modulates the stability and the level of translation of bound mRNA molecules (PubMed:12509468, PubMed:16010978, PubMed:7567451, PubMed:8264621).
  • Sequence similarities

    Belongs to the RRM HNRPC family. RALY subfamily.
    Contains 1 RRM (RNA recognition motif) domain.
  • Post-translational
    modifications

    Phosphorylated on Ser-260 and Ser-299 in resting cells. Phosphorylated on Ser-253 and on 1 serine residue in the poly-Ser stretch at position 238 in response to hydrogen peroxide.
    Sumoylated. Sumoylation reduces affinity for mRNA.
  • Cellular localization

    Nucleus. Component of ribonucleosomes.
  • Information by UniProt
  • Database links

  • Alternative names

    • C1 antibody
    • C2 antibody
    • Heterogeneous nuclear ribonucleoprotein C (C1/C2) antibody
    • Heterogeneous nuclear ribonucleoprotein C antibody
    • Heterogeneous nuclear ribonucleoproteins C1/C2 antibody
    • HNRNP antibody
    • hnRNP C1 / hnRNP C2 antibody
    • hnRNP C1/C2 antibody
    • Hnrnpc antibody
    • HNRPC antibody
    • HNRPC_HUMAN antibody
    • MGC104306 antibody
    • MGC105117 antibody
    • MGC117353 antibody
    • MGC131677 antibody
    • Nuclear ribonucleoprotein particle C1 protein antibody
    • Nuclear ribonucleoprotein particle C2 protein antibody
    • SNRPC antibody
    see all

Images

  • ab128049 at 1/100 staining hnRNP C1+C2/HNRNPC in 4% paraformaldehyde fixed, 0.1% Triton X-100 permeabilized HeLa cells by Immunocytochemistry.

  • 2 µg of ab128049 was added to aliquots of cleared HeLa cell lysates from transfected Human cell lines and incubated for 2 hr at 4°C. Protein-G Dynabeads were then added, and lysates were incubated for additional 2 hr at 4°C to capture the IgG-protein complex. Anti-V5 antibody was used in parallel as a positive control. As a negative control, primary antibody was excluded.
    Lane 1: Cell Lysate control
    Lane 2: Cell lysate with ab128049
    Lane 3: Cell lysate without ab128049
    Lane 4: V5 tag control
  • ChIP was successfully performed using ab128049.
    Lane 1: Input
    Lane 2: IgG
    Lane 3: First elution
    Lane 4: Second elution
  • Quantitative analysis of ChIPd DNA species.
    Left: Distribution and abundance of ChIPd DNAs.
    Right: Computer-regenerated image of negative control and anti-HNRPC-ChIPd DNA.

References

ab128049 has not yet been referenced specifically in any publications.

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