Product nameAnti-hnRNP C1 + C2/HNRNPC antibody [55.1_3A9]
See all hnRNP C1 + C2/HNRNPC primary antibodies
DescriptionMouse monoclonal [55.1_3A9] to hnRNP C1 + C2/HNRNPC
Specificityab128049 specificity has been determined by protein microarray.
Tested applicationsSuitable for: WB, IP, ChIP, ICC/IFmore details
Species reactivityReacts with: Human
Tissue, cells or virus corresponding to Human hnRNP C1 + C2/HNRNPC.
- HeLa cells.
This product was previously labelled as hnRNP C1 + C2
Storage instructionsShipped at 4°C. Store at -20ºC.
Storage bufferPreservative: 0.02% Sodium azide
Constituent: 99% PBS
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab128049 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 33 kDa.|
|IP||Use a concentration of 4 µg/ml.|
|ChIP||Use at an assay dependent dilution.|
|ICC/IF||1/100. 4% paraformaldehyde fixed, 0.1% Triton X-100 permeabilized|
FunctionBinds pre-mRNA and nucleates the assembly of 40S hnRNP particles (PubMed:8264621). Specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs, a modification present at internal sites of mRNAs that affects mRNA splicing, processing and stability. M6A alters the local structure in mRNAs and long non-coding RNAs (lncRNAs) via a mechanism named 'm(6)A-switch' to facilitate binding of HNRNPC, leading to regulation of mRNA splicing (PubMed:25719671). Single HNRNPC tetramers bind 230-240 nucleotides. Trimers of HNRNPC tetramers bind 700 nucleotides. May play a role in the early steps of spliceosome assembly and pre-mRNA splicing. Interacts with poly-U tracts in the 3'-UTR or 5'-UTR of mRNA and modulates the stability and the level of translation of bound mRNA molecules (PubMed:12509468, PubMed:16010978, PubMed:7567451, PubMed:8264621).
Sequence similaritiesBelongs to the RRM HNRPC family. RALY subfamily.
Contains 1 RRM (RNA recognition motif) domain.
modificationsPhosphorylated on Ser-260 and Ser-299 in resting cells. Phosphorylated on Ser-253 and on 1 serine residue in the poly-Ser stretch at position 238 in response to hydrogen peroxide.
Sumoylated. Sumoylation reduces affinity for mRNA.
Cellular localizationNucleus. Component of ribonucleosomes.
- Information by UniProt
- C1 antibody
- C2 antibody
- Heterogeneous nuclear ribonucleoprotein C (C1/C2) antibody
ab128049 at 1/100 staining hnRNP C1+C2/HNRNPC in 4% paraformaldehyde fixed, 0.1% Triton X-100 permeabilized HeLa cells by Immunocytochemistry.
2 µg of ab128049 was added to aliquots of cleared HeLa cell lysates from transfected Human cell lines and incubated for 2 hr at 4°C. Protein-G Dynabeads were then added, and lysates were incubated for additional 2 hr at 4°C to capture the IgG-protein complex. Anti-V5 antibody was used in parallel as a positive control. As a negative control, primary antibody was excluded.
Lane 1: Cell Lysate control
Lane 2: Cell lysate with ab128049
Lane 3: Cell lysate without ab128049
Lane 4: V5 tag control
ChIP was successfully performed using ab128049.
Lane 1: Input
Lane 2: IgG
Lane 3: First elution
Lane 4: Second elution
Quantitative analysis of ChIPd DNA species.
Left: Distribution and abundance of ChIPd DNAs.
Right: Computer-regenerated image of negative control and anti-HNRPC-ChIPd DNA.
ab128049 has not yet been referenced specifically in any publications.