Overview

  • Product name

    Anti-hnRNP C1 + C2/HNRNPC antibody
    See all hnRNP C1 + C2/HNRNPC primary antibodies
  • Description

    Rabbit polyclonal to hnRNP C1 + C2/HNRNPC
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Cow
  • Immunogen

    Recombinant fragment corresponding to Human hnRNP C1 + C2/HNRNPC aa 1-152. (AAH03394)
    Database link: P07910

  • Positive control

    • WB: 293T, A431, HeLa, HepG2, MOLT4 or Raji lysates ICC/IF: HeLa cells IHC-P: Cal27 Xenograft
  • General notes

     This product was previously labelled as hnRNP C1 + C2

     

Properties

Applications

Our Abpromise guarantee covers the use of ab97541 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 32 kDa.
IHC-P 1/100 - 1/250.
ICC/IF 1/100 - 1/200.

Target

  • Function

    Binds pre-mRNA and nucleates the assembly of 40S hnRNP particles (PubMed:8264621). Specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs, a modification present at internal sites of mRNAs that affects mRNA splicing, processing and stability. M6A alters the local structure in mRNAs and long non-coding RNAs (lncRNAs) via a mechanism named 'm(6)A-switch' to facilitate binding of HNRNPC, leading to regulation of mRNA splicing (PubMed:25719671). Single HNRNPC tetramers bind 230-240 nucleotides. Trimers of HNRNPC tetramers bind 700 nucleotides. May play a role in the early steps of spliceosome assembly and pre-mRNA splicing. Interacts with poly-U tracts in the 3'-UTR or 5'-UTR of mRNA and modulates the stability and the level of translation of bound mRNA molecules (PubMed:12509468, PubMed:16010978, PubMed:7567451, PubMed:8264621).
  • Sequence similarities

    Belongs to the RRM HNRPC family. RALY subfamily.
    Contains 1 RRM (RNA recognition motif) domain.
  • Post-translational
    modifications

    Phosphorylated on Ser-260 and Ser-299 in resting cells. Phosphorylated on Ser-253 and on 1 serine residue in the poly-Ser stretch at position 238 in response to hydrogen peroxide.
    Sumoylated. Sumoylation reduces affinity for mRNA.
  • Cellular localization

    Nucleus. Component of ribonucleosomes.
  • Information by UniProt
  • Database links

  • Alternative names

    • C1 antibody
    • C2 antibody
    • Heterogeneous nuclear ribonucleoprotein C (C1/C2) antibody
    • Heterogeneous nuclear ribonucleoprotein C antibody
    • Heterogeneous nuclear ribonucleoproteins C1/C2 antibody
    • HNRNP antibody
    • hnRNP C1 / hnRNP C2 antibody
    • hnRNP C1/C2 antibody
    • Hnrnpc antibody
    • HNRPC antibody
    • HNRPC_HUMAN antibody
    • MGC104306 antibody
    • MGC105117 antibody
    • MGC117353 antibody
    • MGC131677 antibody
    • Nuclear ribonucleoprotein particle C1 protein antibody
    • Nuclear ribonucleoprotein particle C2 protein antibody
    • SNRPC antibody
    see all

Images

  • Anti-hnRNP C1 + C2/HNRNPC antibody (ab97541) at 1/1000 dilution + Raji whole cell lysate at 30 µg

    Predicted band size: 32 kDa



    12% SDS PAGE
  • Immunohistochemical analysis of hnRNP C1 + C2/HNRNPC in paraffin embedded Cal27 Xenograft, using ab97541 at a 1/100 dilution.

  • Immunofluorescence analysis of hnRNP C1 + C2/HNRNPC in paraformaldehyde fixed HeLa, using ab97541 at a 1/200 dilution.
    Lower image is merged with DNA probe.

References

This product has been referenced in:

  • Varjak M  et al. Magnetic fractionation and proteomic dissection of cellular organelles occupied by the late replication complexes of Semliki Forest virus. J Virol 87:10295-312 (2013). Read more (PubMed: 23864636) »
See 1 Publication for this product

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