Product nameAnti-hnRNP C1 + C2/HNRNPC antibody [EP3034Y]
See all hnRNP C1 + C2/HNRNPC primary antibodies
DescriptionRabbit monoclonal [EP3034Y] to hnRNP C1 + C2/HNRNPC
Tested applicationsSuitable for: WB, IHC-P, ICCmore details
Unsuitable for: Flow Cyt or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human hnRNP C1 + C2/HNRNPC (N terminal). The exact sequence is proprietary.
- HeLa, 293T, MCF7 and human cerebellum lysates; human breast carcinoma tissue.
This product was previously labelled as hnRNP C1 + C2
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab75822 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/50000 - 1/100000. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|ICC||1/100 - 1/250.|
FunctionBinds pre-mRNA and nucleates the assembly of 40S hnRNP particles (PubMed:8264621). Specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs, a modification present at internal sites of mRNAs that affects mRNA splicing, processing and stability. M6A alters the local structure in mRNAs and long non-coding RNAs (lncRNAs) via a mechanism named 'm(6)A-switch' to facilitate binding of HNRNPC, leading to regulation of mRNA splicing (PubMed:25719671). Single HNRNPC tetramers bind 230-240 nucleotides. Trimers of HNRNPC tetramers bind 700 nucleotides. May play a role in the early steps of spliceosome assembly and pre-mRNA splicing. Interacts with poly-U tracts in the 3'-UTR or 5'-UTR of mRNA and modulates the stability and the level of translation of bound mRNA molecules (PubMed:12509468, PubMed:16010978, PubMed:7567451, PubMed:8264621).
Sequence similaritiesBelongs to the RRM HNRPC family. RALY subfamily.
Contains 1 RRM (RNA recognition motif) domain.
modificationsPhosphorylated on Ser-260 and Ser-299 in resting cells. Phosphorylated on Ser-253 and on 1 serine residue in the poly-Ser stretch at position 238 in response to hydrogen peroxide.
Sumoylated. Sumoylation reduces affinity for mRNA.
Cellular localizationNucleus. Component of ribonucleosomes.
- Information by UniProt
- C1 antibody
- C2 antibody
- Heterogeneous nuclear ribonucleoprotein C (C1/C2) antibody
ab75822 at 1/100 dilution staining hnRNP C1 + C2/HNRNPC in human breast carcinoma by Immunohistochemistry, Paraffin-embedded tissue.
All lanes : Anti-hnRNP C1 + C2/HNRNPC antibody [EP3034Y] (ab75822) at 1/100000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : 293T cell lysate
Lane 3 : MCF7 cell lysate
Lane 4 : human cerebellum lysate
Lysates/proteins at 10 µg per lane.
All lanes : goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 34 kDa
Observed band size: 34 kDa
This product has been referenced in:
- Rao GK et al. T cell LFA-1-induced proinflammatory mRNA stabilization is mediated by the p38 pathway kinase MK2 in a process regulated by hnRNPs C, H1 and K. PLoS One 13:e0201103 (2018). Read more (PubMed: 30048492) »
- Akhrymuk I et al. Evasion of the innate immune response: the Old World alphavirus nsP2 protein induces rapid degradation of Rpb1, a catalytic subunit of RNA polymerase II. J Virol 86:7180-91 (2012). WB . Read more (PubMed: 22514352) »