Product nameAnti-hnRNP C1 + C2/HNRNPC antibody [EPNCIR152]
See all hnRNP C1 + C2/HNRNPC primary antibodies
DescriptionRabbit monoclonal [EPNCIR152] to hnRNP C1 + C2/HNRNPC
SpecificityThis antibody is specific to isoform C2.
Tested applicationsSuitable for: WB, IHC-P, ICC/IF, IP, Flow Cytmore details
Species reactivityReacts with: Mouse, Rat, Human
corresponding to Human hnRNP C1 + C2/HNRNPC aa 100-200.
Database link: P07910
- WB: HeLa, MCF7, HepG2, K562, and 293T cell lysates IHC-P: Human kidney and Human thyroid carcinoma tissues
This antibody was developed as part of a collaboration between the National Cancer Institute's Center for Cancer Research and the lab of Mirit Aladjem. View antibodies from NCI Center for Cancer Research Collaboration.
This product was previously labelled as hnRNP C1 + C2.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
- Anti-hnRNP C1 + C2/HNRNPC antibody [EPNCIR152] (Alexa Fluor® 488) (ab208251)
- Anti-hnRNP C1 + C2/HNRNPC antibody [EPNCIR152] (Alexa Fluor® 647) (ab208765)
- Anti-hnRNP C1 + C2/HNRNPC antibody [EPNCIR152] (Alexa Fluor® 405) (ab210025)
- Anti-hnRNP C1 + C2/HNRNPC antibody [EPNCIR152] - BSA and Azide free (ab248575)
Our Abpromise guarantee covers the use of ab133607 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Detects a band of approximately 42 kDa (predicted molecular weight: 33 kDa).|
|IHC-P||1/250 - 1/500.|
|ICC/IF||1/250 - 1/500.|
|IP||1/10 - 1/100.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionBinds pre-mRNA and nucleates the assembly of 40S hnRNP particles (PubMed:8264621). Specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs, a modification present at internal sites of mRNAs that affects mRNA splicing, processing and stability. M6A alters the local structure in mRNAs and long non-coding RNAs (lncRNAs) via a mechanism named 'm(6)A-switch' to facilitate binding of HNRNPC, leading to regulation of mRNA splicing (PubMed:25719671). Single HNRNPC tetramers bind 230-240 nucleotides. Trimers of HNRNPC tetramers bind 700 nucleotides. May play a role in the early steps of spliceosome assembly and pre-mRNA splicing. Interacts with poly-U tracts in the 3'-UTR or 5'-UTR of mRNA and modulates the stability and the level of translation of bound mRNA molecules (PubMed:12509468, PubMed:16010978, PubMed:7567451, PubMed:8264621).
Sequence similaritiesBelongs to the RRM HNRPC family. RALY subfamily.
Contains 1 RRM (RNA recognition motif) domain.
modificationsPhosphorylated on Ser-260 and Ser-299 in resting cells. Phosphorylated on Ser-253 and on 1 serine residue in the poly-Ser stretch at position 238 in response to hydrogen peroxide.
Sumoylated. Sumoylation reduces affinity for mRNA.
Cellular localizationNucleus. Component of ribonucleosomes.
- Information by UniProt
- C1 antibody
- C2 antibody
- Heterogeneous nuclear ribonucleoprotein C (C1/C2) antibody
All lanes : Anti-hnRNP C1 + C2/HNRNPC antibody [EPNCIR152] (ab133607) at 1/10000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : MCF7 cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : K562 cell lysate
Lane 5 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 33 kDa
Actual band size : 42 kDa
Immunofluorescence staining of MCF-7 cells with purified ab133607 at a working dilution of 1/500, counter-stained with DAPI. The secondary antibody was an Alexa Fluor® 488 conjugated goat anti-rabbit (ab150077), used at a dilution of 1/1000. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative control is shown in bottom right hand panel - for the negative control, PBS was used instead of the primary antibody.
Immunohistochemical analysis of paraffin embedded Human kidney tissue labelling hnRNP C1 + C2/HNRNPC with ab133607 antibody at a dilution of 1/250.
Immunohistochemical analysis of paraffin embedded Human thyroid carcinoma tissue labelling hnRNP C1 + C2/HNRNPC with ab133607 antibody at a dilution of 1/250.
This product has been referenced in:
- Chen C et al. LNMAT1 promotes lymphatic metastasis of bladder cancer via CCL2 dependent macrophage recruitment. Nat Commun 9:3826 (2018). Read more (PubMed: 30237493) »
- Wu Y et al. Function of HNRNPC in breast cancer cells by controlling the dsRNA-induced interferon response. EMBO J 37:N/A (2018). Read more (PubMed: 30158112) »