Recombinant
RabMAb

Recombinant Anti-hnRNP C1 + C2/HNRNPC antibody [EPNCIR152] (ab133607)

Overview

  • Product name

    Anti-hnRNP C1 + C2/HNRNPC antibody [EPNCIR152]
    See all hnRNP C1 + C2/HNRNPC primary antibodies
  • Description

    Rabbit monoclonal [EPNCIR152] to hnRNP C1 + C2/HNRNPC
  • Host species

    Rabbit
  • Specificity

    This antibody is specific to isoform C2.
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, IP, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    corresponding to Human hnRNP C1 + C2/HNRNPC aa 100-200.
    Database link: P07910

  • Positive control

    • WB: HeLa, MCF7, HepG2, K562, and 293T cell lysates IHC-P: Human kidney and Human thyroid carcinoma tissues
  • General notes

    This antibody was developed as part of a collaboration between the National Cancer Institute's Center for Cancer Research and the lab of Mirit Aladjem. View antibodies from NCI Center for Cancer Research Collaboration.

    This product was previously labelled as hnRNP C1 + C2.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab133607 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 42 kDa (predicted molecular weight: 33 kDa).
IHC-P 1/250 - 1/500.
ICC/IF 1/250 - 1/500.
IP 1/10 - 1/100.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    Binds pre-mRNA and nucleates the assembly of 40S hnRNP particles (PubMed:8264621). Specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs, a modification present at internal sites of mRNAs that affects mRNA splicing, processing and stability. M6A alters the local structure in mRNAs and long non-coding RNAs (lncRNAs) via a mechanism named 'm(6)A-switch' to facilitate binding of HNRNPC, leading to regulation of mRNA splicing (PubMed:25719671). Single HNRNPC tetramers bind 230-240 nucleotides. Trimers of HNRNPC tetramers bind 700 nucleotides. May play a role in the early steps of spliceosome assembly and pre-mRNA splicing. Interacts with poly-U tracts in the 3'-UTR or 5'-UTR of mRNA and modulates the stability and the level of translation of bound mRNA molecules (PubMed:12509468, PubMed:16010978, PubMed:7567451, PubMed:8264621).
  • Sequence similarities

    Belongs to the RRM HNRPC family. RALY subfamily.
    Contains 1 RRM (RNA recognition motif) domain.
  • Post-translational
    modifications

    Phosphorylated on Ser-260 and Ser-299 in resting cells. Phosphorylated on Ser-253 and on 1 serine residue in the poly-Ser stretch at position 238 in response to hydrogen peroxide.
    Sumoylated. Sumoylation reduces affinity for mRNA.
  • Cellular localization

    Nucleus. Component of ribonucleosomes.
  • Information by UniProt
  • Database links

  • Alternative names

    • C1 antibody
    • C2 antibody
    • Heterogeneous nuclear ribonucleoprotein C (C1/C2) antibody
    • Heterogeneous nuclear ribonucleoprotein C antibody
    • Heterogeneous nuclear ribonucleoproteins C1/C2 antibody
    • HNRNP antibody
    • hnRNP C1 / hnRNP C2 antibody
    • hnRNP C1/C2 antibody
    • Hnrnpc antibody
    • HNRPC antibody
    • HNRPC_HUMAN antibody
    • MGC104306 antibody
    • MGC105117 antibody
    • MGC117353 antibody
    • MGC131677 antibody
    • Nuclear ribonucleoprotein particle C1 protein antibody
    • Nuclear ribonucleoprotein particle C2 protein antibody
    • SNRPC antibody
    see all

Images

  • All lanes : Anti-hnRNP C1 + C2/HNRNPC antibody [EPNCIR152] (ab133607) at 1/10000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : MCF7 cell lysate
    Lane 3 : HepG2 cell lysate
    Lane 4 : K562 cell lysate
    Lane 5 : 293T cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 33 kDa



    Actual band size : 42 kDa
  • Immunofluorescence staining of MCF-7 cells with purified ab133607 at a working dilution of 1/500, counter-stained with DAPI. The secondary antibody was an Alexa Fluor® 488 conjugated goat anti-rabbit (ab150077), used at a dilution of 1/1000. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative control is shown in bottom right hand panel - for the negative control, PBS was used instead of the primary antibody.

  • Immunohistochemical analysis of paraffin embedded Human kidney tissue labelling hnRNP C1 + C2/HNRNPC with ab133607 antibody at a dilution of 1/250.

  • Immunohistochemical analysis of paraffin embedded Human thyroid carcinoma tissue labelling hnRNP C1 + C2/HNRNPC with ab133607 antibody at a dilution of 1/250.

References

This product has been referenced in:

  • Chen C  et al. LNMAT1 promotes lymphatic metastasis of bladder cancer via CCL2 dependent macrophage recruitment. Nat Commun 9:3826 (2018). Read more (PubMed: 30237493) »
  • Wu Y  et al. Function of HNRNPC in breast cancer cells by controlling the dsRNA-induced interferon response. EMBO J 37:N/A (2018). Read more (PubMed: 30158112) »
See all 4 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (human fibroblasts)
Total protein in input
500 µg
Immuno-precipitation step
Protein A
Specification
human fibroblasts

Abcam user community

Verified customer

Submitted Jan 05 2015

Application
Western blot
Sample
Human Cell lysate - whole cell (human fibroblasts)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
15 µg
Specification
human fibroblasts
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Dec 26 2014

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