Recombinant Anti-hnRNP C1/C2 antibody [EPNCIR152] (ab133607)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPNCIR152] to hnRNP C1/C2
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-hnRNP C1/C2 antibody [EPNCIR152]
See all hnRNP C1/C2 primary antibodies -
Description
Rabbit monoclonal [EPNCIR152] to hnRNP C1/C2 -
Host species
Rabbit -
Specificity
This antibody is specific to isoform C2. -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa, MCF7, HepG2, K562, and 293T cell lysates IHC-P: Human kidney and Human thyroid carcinoma tissues
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General notes
This antibody was developed as part of a collaboration between the National Cancer Institute's Center for Cancer Research and the lab of Mirit Aladjem. View antibodies from NCI Center for Cancer Research Collaboration.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPNCIR152 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab133607 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/10000 - 1/50000. Detects a band of approximately 42 kDa (predicted molecular weight: 33 kDa).
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IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
1/250 - 1/500.
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Notes |
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WB
1/10000 - 1/50000. Detects a band of approximately 42 kDa (predicted molecular weight: 33 kDa). |
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/250 - 1/500. |
Target
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Function
Binds pre-mRNA and nucleates the assembly of 40S hnRNP particles (PubMed:8264621). Specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs, a modification present at internal sites of mRNAs that affects mRNA splicing, processing and stability. M6A alters the local structure in mRNAs and long non-coding RNAs (lncRNAs) via a mechanism named 'm(6)A-switch' to facilitate binding of HNRNPC, leading to regulation of mRNA splicing (PubMed:25719671). Single HNRNPC tetramers bind 230-240 nucleotides. Trimers of HNRNPC tetramers bind 700 nucleotides. May play a role in the early steps of spliceosome assembly and pre-mRNA splicing. Interacts with poly-U tracts in the 3'-UTR or 5'-UTR of mRNA and modulates the stability and the level of translation of bound mRNA molecules (PubMed:12509468, PubMed:16010978, PubMed:7567451, PubMed:8264621). -
Sequence similarities
Belongs to the RRM HNRPC family. RALY subfamily.
Contains 1 RRM (RNA recognition motif) domain. -
Post-translational
modificationsPhosphorylated on Ser-260 and Ser-299 in resting cells. Phosphorylated on Ser-253 and on 1 serine residue in the poly-Ser stretch at position 238 in response to hydrogen peroxide.
Sumoylated. Sumoylation reduces affinity for mRNA. -
Cellular localization
Nucleus. Component of ribonucleosomes. - Information by UniProt
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Database links
- Entrez Gene: 3183 Human
- Entrez Gene: 15381 Mouse
- Entrez Gene: 290046 Rat
- Omim: 164020 Human
- SwissProt: P07910 Human
- SwissProt: Q9Z204 Mouse
- SwissProt: P17132 Rat
- Unigene: 508848 Human
see all -
Alternative names
- C1 antibody
- C2 antibody
- Heterogeneous nuclear ribonucleoprotein C (C1/C2) antibody
see all
Images
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All lanes : Anti-hnRNP C1/C2 antibody [EPNCIR152] (ab133607) at 1/10000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : MCF7 cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : K562 cell lysate
Lane 5 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 33 kDa
Actual band size : 42 kDa -
Immunofluorescence staining of MCF-7 cells with purified ab133607 at a working dilution of 1/500, counter-stained with DAPI. The secondary antibody was an Alexa Fluor® 488 conjugated goat anti-rabbit (ab150077), used at a dilution of 1/1000. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative control is shown in bottom right hand panel - for the negative control, PBS was used instead of the primary antibody.
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Immunohistochemical analysis of paraffin embedded Human kidney tissue labelling hnRNP C1/C2 with ab133607 antibody at a dilution of 1/250.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin embedded Human thyroid carcinoma tissue labelling hnRNP C1/C2 with ab133607 antibody at a dilution of 1/250.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (9)
ab133607 has been referenced in 9 publications.
- Hu X et al. Survey of the binding preferences of RNA-binding proteins to RNA editing events. Genome Biol 23:169 (2022). PubMed: 35927743
- Chen Y et al. Knockdown of LINC00662 represses AK4 and attenuates radioresistance of oral squamous cell carcinoma. Cancer Cell Int 20:244 (2020). PubMed: 32549791
- Hiltunen AE et al. Variant in NHLRC2 leads to increased hnRNP C2 in developing neurons and the hippocampus of a mouse model of FINCA disease. Mol Med 26:123 (2020). PubMed: 33297935
- Theurillat I et al. Extensive SUMO Modification of Repressive Chromatin Factors Distinguishes Pluripotent from Somatic Cells. Cell Rep 32:108146 (2020). PubMed: 32937131
- Fu K et al. Biological and RNA regulatory function of MOV10 in mammalian germ cells. BMC Biol 17:39 (2019). PubMed: 31088452
- Chen C et al. LNMAT1 promotes lymphatic metastasis of bladder cancer via CCL2 dependent macrophage recruitment. Nat Commun 9:3826 (2018). PubMed: 30237493
- Wu Y et al. Function of HNRNPC in breast cancer cells by controlling the dsRNA-induced interferon response. EMBO J 37:N/A (2018). PubMed: 30158112
- Roundtree IA et al. YTHDC1 mediates nuclear export of N6-methyladenosine methylated mRNAs. Elife 6:N/A (2017). PubMed: 28984244
- Sun DQ et al. Overexpression of hnRNPC2 induces multinucleation by repression of Aurora B in hepatocellular carcinoma cells. Oncol Lett 5:1243-1249 (2013). WB, IP ; Human . PubMed: 23599772