Product nameAnti-hnRNP C1 + C2 antibody [4F4]
See all hnRNP C1 + C2 primary antibodies
DescriptionMouse monoclonal [4F4] to hnRNP C1 + C2
SpecificityThis antibody recognizes the two hnRNP C proteins of 41kDa (C1) and 43kDa (C2) in human cells, and corresponding proteins in monkey hamster & chicken.
Tested applicationsSuitable for: ICC/IF, IP, IHC-P, ELISA, WBmore details
Species reactivityReacts with: Mouse, Chicken, Hamster, Human, Monkey
RNP’s eluted from oligo (dT) cellulose column
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.1% Sodium azide
Concentration information loading...
PurityProtein A purified
Purification notesProtein A purified from tissue culture supernatant.
Our Abpromise guarantee covers the use of ab10294 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration.|
|IHC-P||Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|ELISA||Use at an assay dependent concentration.|
|WB||1/1000. Detects a band of approximately 36 kDa (predicted molecular weight: 41,43 kDa). Human nuclear HEK 293A lysate (see Abreview). Detects isoforms at molecular weight approximately 41,43 kDa.|
FunctionBinds pre-mRNA and nucleates the assembly of 40S hnRNP particles (PubMed:8264621). Specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs, a modification present at internal sites of mRNAs that affects mRNA splicing, processing and stability. M6A alters the local structure in mRNAs and long non-coding RNAs (lncRNAs) via a mechanism named 'm(6)A-switch' to facilitate binding of HNRNPC, leading to regulation of mRNA splicing (PubMed:25719671). Single HNRNPC tetramers bind 230-240 nucleotides. Trimers of HNRNPC tetramers bind 700 nucleotides. May play a role in the early steps of spliceosome assembly and pre-mRNA splicing. Interacts with poly-U tracts in the 3'-UTR or 5'-UTR of mRNA and modulates the stability and the level of translation of bound mRNA molecules (PubMed:12509468, PubMed:16010978, PubMed:7567451, PubMed:8264621).
Sequence similaritiesBelongs to the RRM HNRPC family. RALY subfamily.
Contains 1 RRM (RNA recognition motif) domain.
modificationsPhosphorylated on Ser-260 and Ser-299 in resting cells. Phosphorylated on Ser-253 and on 1 serine residue in the poly-Ser stretch at position 238 in response to hydrogen peroxide.
Sumoylated. Sumoylation reduces affinity for mRNA.
Cellular localizationNucleus. Component of ribonucleosomes.
- Information by UniProt
- C1 antibody
- C2 antibody
- Heterogeneous nuclear ribonucleoprotein C (C1/C2) antibody
All lanes : Anti-hnRNP C1 + C2 antibody [4F4] (ab10294) at 1/1000 dilution
Lane 1 : Nuclear lysate, HEK293A cells
Lane 2 : Cytoplasmic lysate, HEK293A cells
Lysates/proteins at 30 µg per lane.
All lanes : HRP conjugated ImmunoPure Goat Anti-Mouse IgG (H+L) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 41,43 kDa
Observed band size: 42,43 kDa why is the actual band size different from the predicted?
Exposure time: 1 second
Lysates were run on a 10% reducing gel, transferred to PVDF membrane and then incubated with primary antibody overnight. The HRP secondary antibody was incubated for 1 hour at room temperature and then the blot was visualized with ECL+. Cellular fractionation was achieved by Dounce homogenization. Cytoplasmic fraction was achieved by ultra-centrifugation.
ab10294 (1µg/ml) staining hnRNP in human placenta, using an automated system (DAKO Autostainer Plus). Using this protocol there is strong nuclear staining.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer citrate pH6.1 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
This product has been referenced in:
- Montellese C et al. Poly(A)-specific ribonuclease is a nuclear ribosome biogenesis factor involved in human 18S rRNA maturation. Nucleic Acids Res 45:6822-6836 (2017). Read more (PubMed: 28402503) »
- Sharma NR et al. Cell Type- and Tissue Context-dependent Nuclear Distribution of Human Ago2. J Biol Chem 291:2302-9 (2016). Read more (PubMed: 26699195) »