Overview

  • Product name
    Anti-hnRNP C1 + C2 (phospho S260) antibody
    See all hnRNP C1 + C2 primary antibodies
  • Description
    Rabbit polyclonal to hnRNP C1 + C2 (phospho S260)
  • Host species
    Rabbit
  • Specificity
    ab61796 detects endogenous levels of hnRNP C1 + C2 only when phosphorylated at serine 260.
  • Tested applications
    Suitable for: ELISA, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic phosphopeptide derived from human hnRNP C1 + C2 around the phosphorylation site of serine 260 (D-D-SP-A-E).

  • Positive control
    • Human brain tissue and 293 cell extracts treated with H2O2 (100uM, 15mins).

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer
    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab61796 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab61796 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/20000.
IHC-P 1/50 - 1/100.
WB 1/500 - 1/1000. Detects a band of approximately 40, 45 kDa (predicted molecular weight: 34 kDa).

Target

  • Function
    Binds pre-mRNA and nucleates the assembly of 40S hnRNP particles (PubMed:8264621). Specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs, a modification present at internal sites of mRNAs that affects mRNA splicing, processing and stability. M6A alters the local structure in mRNAs and long non-coding RNAs (lncRNAs) via a mechanism named 'm(6)A-switch' to facilitate binding of HNRNPC, leading to regulation of mRNA splicing (PubMed:25719671). Single HNRNPC tetramers bind 230-240 nucleotides. Trimers of HNRNPC tetramers bind 700 nucleotides. May play a role in the early steps of spliceosome assembly and pre-mRNA splicing. Interacts with poly-U tracts in the 3'-UTR or 5'-UTR of mRNA and modulates the stability and the level of translation of bound mRNA molecules (PubMed:12509468, PubMed:16010978, PubMed:7567451, PubMed:8264621).
  • Sequence similarities
    Belongs to the RRM HNRPC family. RALY subfamily.
    Contains 1 RRM (RNA recognition motif) domain.
  • Post-translational
    modifications
    Phosphorylated on Ser-260 and Ser-299 in resting cells. Phosphorylated on Ser-253 and on 1 serine residue in the poly-Ser stretch at position 238 in response to hydrogen peroxide.
    Sumoylated. Sumoylation reduces affinity for mRNA.
  • Cellular localization
    Nucleus. Component of ribonucleosomes.
  • Information by UniProt
  • Database links
  • Alternative names
    • C1 antibody
    • C2 antibody
    • Heterogeneous nuclear ribonucleoprotein C (C1/C2) antibody
    • Heterogeneous nuclear ribonucleoprotein C antibody
    • Heterogeneous nuclear ribonucleoproteins C1/C2 antibody
    • HNRNP antibody
    • hnRNP C1 / hnRNP C2 antibody
    • hnRNP C1/C2 antibody
    • Hnrnpc antibody
    • HNRPC antibody
    • HNRPC_HUMAN antibody
    • MGC104306 antibody
    • MGC105117 antibody
    • MGC117353 antibody
    • MGC131677 antibody
    • Nuclear ribonucleoprotein particle C1 protein antibody
    • Nuclear ribonucleoprotein particle C2 protein antibody
    • SNRPC antibody
    see all

Images

  • All lanes : Anti-hnRNP C1 + C2 (phospho S260) antibody (ab61796) at 1/500 dilution

    Lane 1 : 293 cell extracts treated
    with H2O2 (100uM, 15mins)
    Lane 2 : 293 cell extracts treated
    with H2O2 (100uM, 15mins) with immunising peptide at 5 µg

    Lysates/proteins at 5 µg per lane.

    Predicted band size: 34 kDa
    Observed band size: 45 kDa (why is the actual band size different from the predicted?)
    Additional bands at: 40 kDa. We are unsure as to the identity of these extra bands.

  • ab61796 at 1/50 - 1/100 dilution staining hnRNP C1 + C2 in human brain Left apical lobe by Immunohistochemistry, Paraffin-embedded tissue, in the absence or presence of the immunising peptide.

References

This product has been referenced in:
  • Chen S  et al. Reduced expression of lamin A/C results in modified cell signaling and metabolism coupled with changes in expression of structural proteins. J Proteome Res 8:5196-211 (2009). Read more (PubMed: 19775189) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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