Anti-hnRNP D/AUF1 antibody (ab168281)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-hnRNP D/AUF1 antibody
See all hnRNP D/AUF1 primary antibodies -
Description
Mouse polyclonal to hnRNP D/AUF1 -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Recombinant full length protein, corresponding to amino acids 1-355 of Human hnRNP D/AUF1 (NP_112738.1).
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Positive control
- hnRNP D/AUF1 transfected 293T cell line; HeLa cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.20
Constituent: 100% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab168281 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Predicted molecular weight: 38 kDa. | |
| ICC/IF | Use a concentration of 10 µg/ml. |
Target
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Function
Binds with high affinity to RNA molecules that contain AU-rich elements (AREs) found within the 3'-UTR of many proto-oncogenes and cytokine mRNAs. Also binds to double- and single-stranded DNA sequences in a specific manner and functions a transcription factor. Each of the RNA-binding domains specifically can bind solely to a single-stranded non-monotonous 5'-UUAG-3' sequence and also weaker to the single-stranded 5'-TTAGGG-3' telomeric DNA repeat. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats. Binding of RRM1 to DNA inhibits the formation of DNA quadruplex structure which may play a role in telomere elongation. May be involved in translationally coupled mRNA turnover. Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain. -
Sequence similarities
Contains 2 RRM (RNA recognition motif) domains. -
Post-translational
modificationsArg-345 is dimethylated, probably to asymmetric dimethylarginine.
Methylated by PRMT1, in an insulin-dependent manner. The PRMT1-mediated methylation regulates tyrosine phosphorylation. -
Cellular localization
Nucleus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Component of ribonucleosomes. - Information by UniProt
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Database links
- Entrez Gene: 3184 Human
- Entrez Gene: 11991 Mouse
- Entrez Gene: 79256 Rat
- GenBank: NP_112738.1 Human
- Omim: 601324 Human
- SwissProt: Q14103 Human
- SwissProt: Q60668 Mouse
- SwissProt: Q9JJ54 Rat
see all -
Alternative names
- ARE binding protein AUFI type A antibody
- AU-rich element RNA-binding protein 1 antibody
- AUF antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-hnRNP D/AUF1 antibody (ab168281)Immunofluorescence analysis of HeLa cells, labeling hnRNP D/AUF1 with ab168281 at 10µg/ml. -
Western blot - Anti-hnRNP D/AUF1 antibody (ab168281)All lanes : Anti-hnRNP D/AUF1 antibody (ab168281) at 1 µg/ml
Lane 1 : hnRNP D/AUF1 transfected 293T lysate
Lane 2 : Non-transfected 293T lysate
Lysates/proteins at 15 µl per lane.
Predicted band size: 38 kDa
Datasheets and documents
References
ab168281 has not yet been referenced specifically in any publications.
