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Overview
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Product nameAnti-hnRNP D/AUF1 antibody
See all hnRNP D/AUF1 primary antibodies -
DescriptionMouse polyclonal to hnRNP D/AUF1
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Host speciesMouse
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Tested applicationsSuitable for: WB, ICC/IFmore details
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Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Recombinant full length protein, corresponding to amino acids 1-355 of Human hnRNP D/AUF1 (NP_112738.1).
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Positive control
- hnRNP D/AUF1 transfected 293T cell line; HeLa cells.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
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Storage bufferpH: 7.20
Constituent: 100% PBS -
Concentration information loading... -
PurityProtein A purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Related Products
Applications
Our Abpromise guarantee covers the use of ab168281 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Predicted molecular weight: 38 kDa. | |
| ICC/IF | Use a concentration of 10 µg/ml. |
Target
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FunctionBinds with high affinity to RNA molecules that contain AU-rich elements (AREs) found within the 3'-UTR of many proto-oncogenes and cytokine mRNAs. Also binds to double- and single-stranded DNA sequences in a specific manner and functions a transcription factor. Each of the RNA-binding domains specifically can bind solely to a single-stranded non-monotonous 5'-UUAG-3' sequence and also weaker to the single-stranded 5'-TTAGGG-3' telomeric DNA repeat. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats. Binding of RRM1 to DNA inhibits the formation of DNA quadruplex structure which may play a role in telomere elongation. May be involved in translationally coupled mRNA turnover. Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain.
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Sequence similaritiesContains 2 RRM (RNA recognition motif) domains.
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Post-translational
modificationsArg-345 is dimethylated, probably to asymmetric dimethylarginine.
Methylated by PRMT1, in an insulin-dependent manner. The PRMT1-mediated methylation regulates tyrosine phosphorylation. -
Cellular localizationNucleus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Component of ribonucleosomes.
- Information by UniProt
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Database links
- Entrez Gene: 3184 Human
- Entrez Gene: 11991 Mouse
- Entrez Gene: 79256 Rat
- GenBank: NP_112738.1 Human
- Omim: 601324 Human
- SwissProt: Q14103 Human
- SwissProt: Q60668 Mouse
- SwissProt: Q9JJ54 Rat
see all -
Alternative names
- ARE binding protein AUFI type A antibody
- AU-rich element RNA-binding protein 1 antibody
- AUF antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-hnRNP D/AUF1 antibody (ab168281)Immunofluorescence analysis of HeLa cells, labeling hnRNP D/AUF1 with ab168281 at 10µg/ml. -
Western blot - Anti-hnRNP D/AUF1 antibody (ab168281)All lanes : Anti-hnRNP D/AUF1 antibody (ab168281) at 1 µg/ml
Lane 1 : hnRNP D/AUF1 transfected 293T lysate
Lane 2 : Non-transfected 293T lysate
Lysates/proteins at 15 µl per lane.
Predicted band size: 38 kDa
References
ab168281 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"