Anti-hnRNP D/AUF1 antibody (ab168281)
Key features and details
- Mouse polyclonal to hnRNP D/AUF1
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-hnRNP D/AUF1 antibody
See all hnRNP D/AUF1 primary antibodies -
Description
Mouse polyclonal to hnRNP D/AUF1 -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Recombinant full length protein, corresponding to amino acids 1-355 of Human hnRNP D/AUF1 (NP_112738.1).
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Positive control
- hnRNP D/AUF1 transfected 293T cell line; HeLa cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.4
Constituent: 100% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab168281 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Predicted molecular weight: 38 kDa. | |
| ICC/IF | Use a concentration of 10 µg/ml. |
Target
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Function
Binds with high affinity to RNA molecules that contain AU-rich elements (AREs) found within the 3'-UTR of many proto-oncogenes and cytokine mRNAs. Also binds to double- and single-stranded DNA sequences in a specific manner and functions a transcription factor. Each of the RNA-binding domains specifically can bind solely to a single-stranded non-monotonous 5'-UUAG-3' sequence and also weaker to the single-stranded 5'-TTAGGG-3' telomeric DNA repeat. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats. Binding of RRM1 to DNA inhibits the formation of DNA quadruplex structure which may play a role in telomere elongation. May be involved in translationally coupled mRNA turnover. Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain. -
Sequence similarities
Contains 2 RRM (RNA recognition motif) domains. -
Post-translational
modificationsArg-345 is dimethylated, probably to asymmetric dimethylarginine.
Methylated by PRMT1, in an insulin-dependent manner. The PRMT1-mediated methylation regulates tyrosine phosphorylation. -
Cellular localization
Nucleus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Component of ribonucleosomes. - Information by UniProt
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Database links
- Entrez Gene: 3184 Human
- Entrez Gene: 11991 Mouse
- Entrez Gene: 79256 Rat
- GenBank: NP_112738.1 Human
- Omim: 601324 Human
- SwissProt: Q14103 Human
- SwissProt: Q60668 Mouse
- SwissProt: Q9JJ54 Rat
see all -
Alternative names
- ARE binding protein AUFI type A antibody
- AU-rich element RNA-binding protein 1 antibody
- AUF antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-hnRNP D/AUF1 antibody (ab168281)Immunofluorescence analysis of HeLa cells, labeling hnRNP D/AUF1 with ab168281 at 10µg/ml.
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Western blot - Anti-hnRNP D/AUF1 antibody (ab168281)All lanes : Anti-hnRNP D/AUF1 antibody (ab168281) at 1 µg/ml
Lane 1 : hnRNP D/AUF1 transfected 293T lysate
Lane 2 : Non-transfected 293T lysate
Lysates/proteins at 15 µl per lane.
Predicted band size: 38 kDa
Protocols
Datasheets and documents
References (0)
ab168281 has not yet been referenced specifically in any publications.
