Anti-hnRNP D/AUF1 antibody (ab168281)
- Datasheet
- References
- Protocols
Overview
-
Product nameAnti-hnRNP D/AUF1 antibody
See all hnRNP D/AUF1 primary antibodies -
DescriptionMouse polyclonal to hnRNP D/AUF1
-
Host speciesMouse
-
Tested applicationsSuitable for: WB, ICC/IFmore details
-
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
-
Immunogen
Recombinant full length protein, corresponding to amino acids 1-355 of Human hnRNP D/AUF1 (NP_112738.1).
-
Positive control
- hnRNP D/AUF1 transfected 293T cell line; HeLa cells.
Properties
-
FormLiquid
-
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
-
Storage bufferpH: 7.20
Constituent: 100% PBS -
Concentration information loading... -
PurityProtein A purified
-
ClonalityPolyclonal
-
IsotypeIgG
-
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
-
Related Products
Applications
Our Abpromise guarantee covers the use of ab168281 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Predicted molecular weight: 38 kDa. | |
| ICC/IF | Use a concentration of 10 µg/ml. |
Target
-
FunctionBinds with high affinity to RNA molecules that contain AU-rich elements (AREs) found within the 3'-UTR of many proto-oncogenes and cytokine mRNAs. Also binds to double- and single-stranded DNA sequences in a specific manner and functions a transcription factor. Each of the RNA-binding domains specifically can bind solely to a single-stranded non-monotonous 5'-UUAG-3' sequence and also weaker to the single-stranded 5'-TTAGGG-3' telomeric DNA repeat. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats. Binding of RRM1 to DNA inhibits the formation of DNA quadruplex structure which may play a role in telomere elongation. May be involved in translationally coupled mRNA turnover. Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain.
-
Sequence similaritiesContains 2 RRM (RNA recognition motif) domains.
-
Post-translational
modificationsArg-345 is dimethylated, probably to asymmetric dimethylarginine.
Methylated by PRMT1, in an insulin-dependent manner. The PRMT1-mediated methylation regulates tyrosine phosphorylation. -
Cellular localizationNucleus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Component of ribonucleosomes.
- Information by UniProt
-
Database links
- Entrez Gene: 3184 Human
- Entrez Gene: 11991 Mouse
- Entrez Gene: 79256 Rat
- GenBank: NP_112738.1 Human
- Omim: 601324 Human
- SwissProt: Q14103 Human
- SwissProt: Q60668 Mouse
- SwissProt: Q9JJ54 Rat
see all -
Alternative names
- ARE binding protein AUFI type A antibody
- AU-rich element RNA-binding protein 1 antibody
- AUF antibody
see all
Images
-
Immunocytochemistry/ Immunofluorescence - Anti-hnRNP D/AUF1 antibody (ab168281)Immunofluorescence analysis of HeLa cells, labeling hnRNP D/AUF1 with ab168281 at 10µg/ml. -
Western blot - Anti-hnRNP D/AUF1 antibody (ab168281)All lanes : Anti-hnRNP D/AUF1 antibody (ab168281) at 1 µg/ml
Lane 1 : hnRNP D/AUF1 transfected 293T lysate
Lane 2 : Non-transfected 293T lysate
Lysates/proteins at 15 µl per lane.
Predicted band size: 38 kDa
Datasheets and documents
References
ab168281 has not yet been referenced specifically in any publications.
