Overview

  • Product name

    Anti-hnRNP D/AUF1 antibody
    See all hnRNP D/AUF1 primary antibodies
  • Description

    Rabbit polyclonal to hnRNP D/AUF1
  • Host species

    Rabbit
  • Specificity

    Detects total hnRNP D/AUF1 levels.
  • Tested applications

    Suitable for: IP, WB, ICC/IF, IHC-P, ELISAmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic non-phosphopeptide (Human) from around the phosphorylation site of serine 83 (NSSPPR)

  • Positive control

    • Lung carcinoma tissue. 293 cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride

    Without Mg2+ and Ca2+
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab61193 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration. PubMed: 20435889
WB 1/500 - 1/1000. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa).
ICC/IF 1/500 - 1/1000.
IHC-P 1/50 - 1/100.
ELISA 1/20000.

Target

  • Function

    Binds with high affinity to RNA molecules that contain AU-rich elements (AREs) found within the 3'-UTR of many proto-oncogenes and cytokine mRNAs. Also binds to double- and single-stranded DNA sequences in a specific manner and functions a transcription factor. Each of the RNA-binding domains specifically can bind solely to a single-stranded non-monotonous 5'-UUAG-3' sequence and also weaker to the single-stranded 5'-TTAGGG-3' telomeric DNA repeat. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats. Binding of RRM1 to DNA inhibits the formation of DNA quadruplex structure which may play a role in telomere elongation. May be involved in translationally coupled mRNA turnover. Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain.
  • Sequence similarities

    Contains 2 RRM (RNA recognition motif) domains.
  • Post-translational
    modifications

    Arg-345 is dimethylated, probably to asymmetric dimethylarginine.
    Methylated by PRMT1, in an insulin-dependent manner. The PRMT1-mediated methylation regulates tyrosine phosphorylation.
  • Cellular localization

    Nucleus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Component of ribonucleosomes.
  • Information by UniProt
  • Database links

  • Alternative names

    • ARE binding protein AUFI type A antibody
    • AU-rich element RNA-binding protein 1 antibody
    • AUF antibody
    • AUF1 antibody
    • AUF1A antibody
    • Heterogeneous nuclear ribonucleoprotein D0 antibody
    • hnRNP D antibody
    • hnRNP D0 antibody
    • Hnrnpd antibody
    • hnRNPD0 antibody
    • HNRPD antibody
    • HNRPD_HUMAN antibody
    • P37 antibody
    see all

Images

  • All lanes : Anti-hnRNP D/AUF1 antibody (ab61193) at 1/500 dilution

    Lane 1 : 293 cell extract
    Lane 2 : 293 cell extract with immunizing peptide

    Predicted band size: 38 kDa
    Observed band size: 38 kDa

  • Immunohistochemical analysis of paraffin embedded human lung carcinoma tissue using ab61193 at 1/50-1/100 dilution. Samples were treated -/+ peptide.
  • ICC/IF image of ab61193 stained Hek293 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab61193, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

This product has been referenced in:

  • Dai W  et al. Berberine Promotes Apoptosis of Colorectal Cancer via Regulation of the Long Non-Coding RNA (lncRNA) Cancer Susceptibility Candidate 2 (CASC2)/AU-Binding Factor 1 (AUF1)/B-Cell CLL/Lymphoma 2 (Bcl-2) Axis. Med Sci Monit 25:730-738 (2019). Read more (PubMed: 30681073) »
  • Wang H  et al. Embryonic lethal abnormal vision proteins and adenine and uridine-rich element mRNAs after global cerebral ischemia and reperfusion in the rat. J Cereb Blood Flow Metab 37:1494-1507 (2017). Read more (PubMed: 27381823) »
See all 12 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Answer

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number xxx with ab50692.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

Read More

Answer

Thank you for your reply.

I'd be happy to send you another vial of ab61193, but unfortunately, we only have the same lot in stock (GR45177-1). I am sorry about that.

Another option would be a replacement with another hnRNP D/AUF1 such as ab50692. Please let me know if that would be a good option for you.
https://www.abcam.com/hnRNP-D-AUF1-antibody-ab50692.html

Alternatively, a credit or refund can be arranged.

I look forward to hear back from you.

Read More

Answer

Thank you for your email and for contacting us. I am sorry to hear this antibody is not providing satisfactory results in WB.

The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality. I appreciate the time you have spent in the laboratory and understand your concerns. It is regrettable the results have not been successful.

Having reviewed the protocol details, I believe this product should have given satisfactory WB results. I am not certain why so many non-specific bands are being obtained. It might bethat you may have received a faulty vial.

I apologize for the inconvenience and am pleased to offer you a free of charge replacement, credit note, or refund in compensation. Please let me know which option would be best for you.

I look forward to hear back from you and resolve this case for you.

Read More

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