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We ordered an AUF1 antibody from your company last week.
( Anti-hnRNP D/AUF1 antibody ) ( ab61193 )
We used it previously for immunohistochemistry ( IHC )on human
sections and its results were pretty good.
Afterwards, we worked with it a trial Western Blot and we detected six
bands between 25 kDa and 75 kDa. Also you noticed just one single band
on 38 kDa in datasheet !
( We run it on proteins which derived from human endometrial stromal
cell culture. We diluted the ab. 1/1000 in milk 10%) ( C=Control ,
E2=Estrogen treatment P4=Progesteron treatment )
We used Peroxidase labeled Anti-Rabbit IgG from Vector Laboratories
as secondary antibody and diluted the sec. ab. 1/5000 in milk 10%.
So I send you WB results ( Results of AUF1 and Actin ) and I look
forward your solution about this situation.
Asked on Feb 28 2012
Thank you for your email and for contacting us. I am sorry to hear this antibody is not providing satisfactory results in WB.
The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality. I appreciate the time you have spent in the laboratory and understand your concerns. It is regrettable the results have not been successful.
Having reviewed the protocol details, I believe this product should have given satisfactory WB results. I am not certain why so many non-specific bands are being obtained. It might bethat you may have received a faulty vial.
I apologize for the inconvenience and am pleased to offer you a free of charge replacement, credit note, or refund in compensation. Please let me know which option would be best for you.
I look forward to hear back from you and resolve this case for you.
Answered on Feb 28 2012