Overview

  • Product name
    Anti-hnRNP F+H antibody [1G11]
  • Description
    Mouse monoclonal [1G11] to hnRNP F+H
  • Host species
    Mouse
  • Specificity
    Human hnRNP F/H.
  • Tested applications
    Suitable for: IHC-P, ELISA, WB, IP, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human, Xenopus laevis
  • Immunogen

    Unfortunately, this information is considered to be commercially sensitive

Properties

Applications

Our Abpromise guarantee covers the use of ab10689 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ELISA Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 53 kDa (hnRNP F), 56 kDa (hnRNP H). (predicted molecular weight: hnRNP F = 49.8 kDa, hnRNP H = 53.9kDa).
IP Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.

Target

  • Relevance
    The hnRNPs belong to a subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins. hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties.
  • Database links
  • Alternative names
    • Heterogeneous nuclear ribonucleoprotein F antibody
    • Heterogeneous nuclear ribonucleoprotein H1 antibody
    • hnRNP F antibody
    • hnRNP H antibody
    • HNRNPF antibody
    • HNRNPH1 antibody
    • HNRPH1 antibody
    • mcs94 1 antibody
    • Nucleolin like protein antibody
    • Nucleolin like protein mcs94 1 antibody
    see all

Images

  • IHC image of ab10689 staining in human kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab10689, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References

This product has been referenced in:
  • Li R  et al. Misregulation of Alternative Splicing in a Mouse Model of Rett Syndrome. PLoS Genet 12:e1006129 (2016). Read more (PubMed: 27352031) »
  • Cooper-Knock J  et al. Antisense RNA foci in the motor neurons of C9ORF72-ALS patients are associated with TDP-43 proteinopathy. Acta Neuropathol 130:63-75 (2015). IHC . Read more (PubMed: 25943887) »
See all 13 Publications for this product

Customer reviews and Q&As

1-7 of 7 Abreviews or Q&A

Answer

Thank you very much for your review of ab10689.

I have added the discount code to our internal notes of your review, so the code is active and can now be redeemed for a free primary antibody in our catalog.

Please let me know if you have any questions or if there is anything else that we can do for you. Have a nice day.

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - nuclear (Brain)
Loading amount
40 µg
Specification
Brain
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jul 20 2012

Answer

DISCOUNT CODE: ***
Expiration date: November 7, 2012

This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for ab10689 with mouse samples and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

Read More

Question
Answer

Thank you for your call today and for your questions.

Sinceab10689 has not been testedwith mouse samples,I can offer a discount off a future purchase if you buy ab10689 now, test itwith mouse samplesand submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be worth 1 free primary antibody.

If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and test ab10689 with mouse samples. I will then send a discount code. This code must be issued before purchasing ab10689 so please wait for my reply before ordering.

2. Purchase ab10689 either by phone, fax, or online (www.abcam.com).

3. Test it with mouse samples.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue. Please remember that submission of the Abreview is sufficient for the discount code to become active.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab10689 turns out to be unsuitable for use with mouse samples, you will still receive the discount on your next purchase after your Abreview has been submitted.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

Read More

Answer

Thank you for contacting Abcam regarding the IF protocol for ab10689. If you are staining cells, I have provided two protocols below, depending on your fixation method.  If you are staining tissue, the protocol is available on the datasheet (under image using DAB detection).  I hope this information is helpful.  Please do not hesitate to contact me if you have any additional questions or concerns. Immunofluorescence protocol - Formaldehyde fixation    1. Collect cells from T.c.unit and remove media from petri dish using suction.    2. Wash with 1x PBS and remove.    3. Incubate cells in pre-warm (37°C) Para-Formaldehyde for 12 minutes at room temperature on an orbital shaker.    4. Remove PFA and incubate in 0.5% Triton X-IOO in 1x PBS for 5 minutes at room temperature.    5. Prepare blocking reagent, this is also the antibody diluent.    6. Wash cells 2x with 1x PBS at room temperature, for 4 minutes/wash on an orbital shaker.    7. Block with 1 % NCS and 1x PBS for 30 minutes at room temperature.    8. Prepare primary antibodies (50ul/coverslip) and moist staining chambers.    9. Wash cells 2x with lx PBS at room temperature and air dry briefly.   10. Incubate with primary antibody for 1 hr at room temperature in the dark in staining chambers. During this time prepare the secondary antibody.   11. Wash cells 5x with 1x PBS (5 beaker changes/5 counts in each beaker)   12. Incubate with secondary antibody for 1 hour at room temperature in the dark in staining chambers.   13. Wash cells 5x with 1x PBS.   14. Mount in Dapi. Solutions (prepare fresh the same day of staining).     * 1x Phosphate buffered saline.     * Blocking reagent: 1% NCS in 1x PBS (use fresh l0x PBS).     * Fixation solution: 3.5% Para formaldehyde. 1.75g PFA in 20 ml d.H20 plus 5 drops 1M NaOH. Stir on a hot plate at 50-60°C until dissolved. Add 4 drops IN HCI and check pH indicator strip. PH should be 7.4. Complete volume with d.H20 to 25ml and add 25ml 2xPBS. Check pH before adding to cover slips. Immunofluorescence protocol - Methanol/acetone fixation    1. Collect cells from T.C.unit and remove media from petri dish using suction.    2. Wash with 1x PBS and remove.    3. Fix cells with cold methanol: acetone 1: 1 for 10 minutes on ice.    4. Prepare blocking reagent, this is also the diluent for the antibodies.    5. Remove fixative and wash cells 3x with Ix PBS at RT, for 4 minutes/wash on orbital shaker.    6. Block with 1% NCS and Ix PBS for 30 minutes at RT.    7. Prepare primary antibodies (50ul/coverslip) and moist staining chambers.    8. Wash cells 2x with 1 x PBS at RT and air dry for approximately 7 minutes.    9. Incubate with primary antibody for 1 hr at RT in the dark in staining chambers.  During this time prepare secondary antibody.   10. Wash cells 5x with 1x PBS (5 beaker changes/5 counts in each beaker)   11. Incubate with secondary antibody for 1 hr at R T in the dark in staining chambers.   12. Wash cells 5x with 1x PBS.   13. Mount in Dapi. Solutions (prepare fresh the same day of staining)     * 1x Phosphate buffered saline.     * Blocking reagent: 1% NCS in 1x PBS (use fresh 10x PBS).     * Fixation solution: methanol:acetone 1: 1 ice cold.  

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunoprecipitation
Sample
Human Cell lysate - other (HeLa cytosolic extract)
Total protein in input
500 µg
Specification
HeLa cytosolic extract
Immuno-precipitation step
Protein G

Abcam user community

Verified customer

Submitted Oct 30 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa cytosolic and nuclear extracts)
Loading amount
50 µg
Specification
HeLa cytosolic and nuclear extracts
Gel Running Conditions
Reduced Denaturing (8% gel)
Blocking step
Milk as blocking agent for 14 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Abcam user community

Verified customer

Submitted Oct 30 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up