Recombinant
RabMAb

Recombinant Anti-hnRNP G antibody [EPR16038] - BSA and Azide free (ab238987)

Overview

  • Product name

    Anti-hnRNP G antibody [EPR16038] - BSA and Azide free
    See all hnRNP G primary antibodies
  • Description

    Rabbit monoclonal [EPR16038] to hnRNP G - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human hnRNP G aa 300 to the C-terminus. The exact sequence is proprietary.
    Database link: P38159

  • Positive control

    • IHC-P: Human transitional cell carcinoma tissue.
  • General notes

    Ab238987 is the carrier-free version of ab190352. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab238987 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab238987 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).
ICC/IF Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Relevance

    Heterogeneous nuclear ribonuclearproteins (hnRNPs) are nucleic acid binding proteins and have critical roles in DNA repair, telomere regulation, and transcriptional gene regulation. hnRNP G has tumor suppressive activity and this RNA binding protein which may be involved in pre-mRNA splicing.
  • Cellular localization

    Nuclear
  • Database links

  • Alternative names

    • Glycoprotein p43 antibody
    • Heterogeneous nuclear ribonucleoprotein G antibody
    • hnRNP G antibody
    • HNRNPG antibody
    • HNRPG antibody
    • RBMX antibody
    • RBMXP1 antibody
    • RBMXRT antibody
    • RNA binding motif protein, X chromosome antibody
    • RNA binding motif protein, X linked antibody
    • RNMX antibody
    see all

Images

  • Immunofluorescent analysis of 4% paraformaldehyde fixed HeLa cells labeling hnRNP G with ab190352 at a 1/250 dilution followed by Goat anti rabbit IgG (Alexa Fluor®555) at a 1/200 dilution (Red). Negative controls and DAPI staining also shown.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab190352).

  • Immunofluorescent analysis of 4% paraformaldehyde fixed A431 cells labeling hnRNP G with ab190352 at a 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor®555) at a 1/200 dilution (Red). Negative controls and DAPI staining also shown.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab190352).

  • Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling hnRNP G with ab190352 at 1/250 dilution. Counter stain Hematoxylin. Negative control also shown lower left.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab190352).

  • Immunohistochemical analysis of paraffin-embedded human transitional cell carcinoma of bladder tissue labeling hnRNP G with ab190352 at 1/250 dilution. Counter stain Hematoxylin. Negative control also shown lower left.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab190352).

References

ab238987 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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