Recombinant Anti-hnRNP G-T antibody [EPR12593] (ab175228)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12593] to hnRNP G-T
- Suitable for: ICC/IF, IP, WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-hnRNP G-T antibody [EPR12593]
See all hnRNP G-T primary antibodies -
Description
Rabbit monoclonal [EPR12593] to hnRNP G-T -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IP, WBmore details
Unsuitable for: Flow Cyt or IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment within Human hnRNP G-T aa 150-350. The exact sequence is proprietary.
Database link: O75526 -
Positive control
- Mouse testis, rat testis, Human seminoma, Jurkat and HeLa lysates; Jurkat cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR12593 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab175228 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
1/100 - 1/250.
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IP |
1/10 - 1/100.
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WB |
1/10000 - 1/50000. Predicted molecular weight: 43 kDa.
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Notes |
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ICC/IF
1/100 - 1/250. |
IP
1/10 - 1/100. |
WB
1/10000 - 1/50000. Predicted molecular weight: 43 kDa. |
Target
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Tissue specificity
Expressed predominantly in spermatocytes and less in round spermatids (at protein level). Expressed in germ cells. -
Sequence similarities
Contains 1 RRM (RNA recognition motif) domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 27288 Human
- Entrez Gene: 76572 Mouse
- Entrez Gene: 365344 Rat
- Omim: 605444 Human
- SwissProt: O75526 Human
- Unigene: 121605 Human
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Alternative names
- Heterogeneous nuclear ribonucleoprotein G T antibody
- hnRNP G T antibody
- hnRNP G-T antibody
see all
Images
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All lanes : Anti-hnRNP G-T antibody [EPR12593] (ab175228) at 1/10000 dilution
Lane 1 : Mouse testis tissue lysate
Lane 2 : Rat testis tissue lysate
Lane 3 : Human seminoma lysate
Lane 4 : Jurkat lysate
Lane 5 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 43 kDa -
Western blot analysis on immunoprecipitation pellet from (1) HeLa cells lysate or (2) 1X PBS (negative control) using ab175228 and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
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Immunofluorescent analysis of Jurkat cells labeling hnRNP G-T with ab175228 at 1/100 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab175228 has not yet been referenced specifically in any publications.