Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12593] to hnRNP G-T
- Suitable for: ICC/IF, IP, WB
- Reacts with: Mouse, Rat, Human
Product nameAnti-hnRNP G-T antibody [EPR12593]
See all hnRNP G-T primary antibodies
DescriptionRabbit monoclonal [EPR12593] to hnRNP G-T
Tested applicationsSuitable for: ICC/IF, IP, WBmore details
Unsuitable for: Flow Cyt or IHC-P
Species reactivityReacts with: Mouse, Rat, Human
Recombinant fragment within Human hnRNP G-T aa 150-350. The exact sequence is proprietary.
Database link: O75526
- Mouse testis, rat testis, Human seminoma, Jurkat and HeLa lysates; Jurkat cells.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab175228 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/100 - 1/250.|
|IP||1/10 - 1/100.|
|WB||1/10000 - 1/50000. Predicted molecular weight: 43 kDa.|
Tissue specificityExpressed predominantly in spermatocytes and less in round spermatids (at protein level). Expressed in germ cells.
Sequence similaritiesContains 1 RRM (RNA recognition motif) domain.
- Information by UniProt
- Heterogeneous nuclear ribonucleoprotein G T antibody
- hnRNP G T antibody
- hnRNP G-T antibody
All lanes : Anti-hnRNP G-T antibody [EPR12593] (ab175228) at 1/10000 dilution
Lane 1 : Mouse testis lysate
Lane 2 : Rat testis lysate
Lane 3 : Human seminoma lysate
Lane 4 : Jurkat lysate
Lane 5 : HeLa lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 43 kDa
Western blot analysis on immunoprecipitation pellet from (1) HeLa cells lysate or (2) 1X PBS (negative control) using ab175228 and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
Immunofluorescent analysis of Jurkat cells labeling hnRNP G-T with ab175228 at 1/100 dilution.
ab175228 has not yet been referenced specifically in any publications.