Recombinant Anti-hnRNP G-T antibody [EPR12593] - BSA and Azide free (ab249879)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12593] to hnRNP G-T - BSA and Azide free
- Suitable for: ICC/IF, IP, WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-hnRNP G-T antibody [EPR12593] - BSA and Azide free
See all hnRNP G-T primary antibodies -
Description
Rabbit monoclonal [EPR12593] to hnRNP G-T - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IP, WBmore details
Unsuitable for: Flow Cyt or IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment within Human hnRNP G-T. The exact sequence is proprietary.
Database link: O75526 -
General notes
ab249879 is the carrier-free version of ab175228.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR12593 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab249879 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use at an assay dependent concentration.
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IP |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 43 kDa.
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Notes |
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ICC/IF
Use at an assay dependent concentration. |
IP
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 43 kDa. |
Target
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Tissue specificity
Expressed predominantly in spermatocytes and less in round spermatids (at protein level). Expressed in germ cells. -
Sequence similarities
Contains 1 RRM (RNA recognition motif) domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 27288 Human
- Entrez Gene: 76572 Mouse
- Entrez Gene: 365344 Rat
- Omim: 605444 Human
- SwissProt: O75526 Human
- Unigene: 121605 Human
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Alternative names
- Heterogeneous nuclear ribonucleoprotein G T antibody
- hnRNP G T antibody
- hnRNP G-T antibody
see all
Images
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All lanes : Anti-hnRNP G-T antibody [EPR12593] (ab175228) at 1/10000 dilution
Lane 1 : Mouse testis tissue lysate
Lane 2 : Rat testis tissue lysate
Lane 3 : Human seminoma lysate
Lane 4 : Jurkat lysate
Lane 5 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 43 kDaThis data was developed using ab175228, the same antibody clone in a different buffer formulation.
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This data was developed using ab175228, the same antibody clone in a different buffer formulation.Western blot analysis on immunoprecipitation pellet from (1) HeLa cells lysate or (2) 1X PBS (negative control) using ab175228 and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab249879 has not yet been referenced specifically in any publications.