Key features and details
- Rabbit polyclonal to hnRNP H
- Suitable for: WB, IP, IHC-Fr, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-hnRNP H antibody
See all hnRNP H primary antibodies
DescriptionRabbit polyclonal to hnRNP H
SpecificityThe antibody was designed against hnRNPH1 and does not recognize hnRNPH3. The epitope recognized by ab10374 maps to a region between residue 400 and the C-terminus (residue 448) of human Heterogeneous Nuclear Ribonucleoprotein H1 using the numbering given in entry NP_005511.1 (GeneID 3187).
Tested applicationsSuitable for: WB, IP, IHC-Fr, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Opossum, Orangutan, Elephant
Immunogen was a synthetic peptide, which represented a portion of the C-terminus of human heterogeneous nuclear ribonucleoprotein H1 (LocusLink ID 3187).
- nuclear lysate from HeLa cells.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAntibodies were affinity purified using the peptide immobilized on solid support.
Our Abpromise guarantee covers the use of ab10374 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/30000. Detects a band of approximately 49 kDa (predicted molecular weight: 53 kDa).|
|IP||Use at 4-8 µg/mg of lysate.|
|IHC-Fr||Use at an assay dependent concentration. PubMed: 24462217|
|IHC-P||Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||1/500. PubMed: 20020773|
FunctionThis protein is a component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. Mediates pre-mRNA alternative splicing regulation. Inhibits, together with CUGBP1, insulin receptor (IR) pre-mRNA exon 11 inclusion in myoblast. Binds to the IR RNA. Binds poly(RG).
Tissue specificityExpressed ubiquitously.
Sequence similaritiesContains 3 RRM (RNA recognition motif) domains.
DomainEach quasi-RRM repeat bound poly(RG), while only the N-terminal QRRM bound poly(RC) and poly(RU). None of the repeats bound detectable amounts of poly(RA).
Cellular localizationNucleus > nucleoplasm.
- Information by UniProt
- DKFZp686A15170 antibody
- Heterogeneous nuclear ribonucleoprotein H antibody
- Heterogeneous nuclear ribonucleoprotein H1 (H) antibody
ab10374 detection of human hnRNPH by Western Blot. Samples: Nuclear extract (NE; 40ab10374 detection of human hnRNPH by Western Blot. Samples: Nuclear extract (NE; 40 µg) from HeLa cells and recombinant human hnRNPH (rH; ~100 ng). Antibody: ab10374 used at the indicated concentrations. Detection: Protein A – HRP with chemiluminescence (20 second exposure after a 20 minute delay to allow signal to decay).
µg) from HeLa cells and recombinant human hnRNPH (rH; ~100 ng). Antibody: ab10374 used at the indicated concentrations. Detection: Protein A – HRP with chemiluminescence (20 second exposure after a 20 minute delay to allow signal to decay).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling hnRNP H with ab10374 at 1/1000 (1µg/ml). Detection: DAB.
ICC/IF image of ab10374 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab10374, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
IHC image of ab10374 staining in human normal cervical carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab10374, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
ab10374 has been referenced in 23 publications.
- Xu S et al. Ai-lncRNA EGOT enhancing autophagy sensitizes paclitaxel cytotoxicity via upregulation of ITPR1 expression by RNA-RNA and RNA-protein interactions in human cancer. Mol Cancer 18:89 (2019). PubMed: 30999914
- Jády BE et al. Dynamic association of human mRNP proteins with mitochondrial tRNAs in the cytosol. RNA 24:1706-1720 (2018). PubMed: 30139801
- Braun S et al. Decoding a cancer-relevant splicing decision in the RON proto-oncogene using high-throughput mutagenesis. Nat Commun 9:3315 (2018). PubMed: 30120239
- Accornero F et al. BEX1 is an RNA-dependent mediator of cardiomyopathy. Nat Commun 8:1875 (2017). PubMed: 29192139
- Subhasitanont P et al. Apigenin inhibits growth and induces apoptosis in human cholangiocarcinoma cells. Oncol Lett 14:4361-4371 (2017). PubMed: 28943950