Overview

  • Product name
    Anti-hnRNP H antibody
    See all hnRNP H primary antibodies
  • Description
    Rabbit polyclonal to hnRNP H
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Chicken, Cow, Xenopus laevis
  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 50-289 of Human hnRNP H (Uniprot ID P31943)

  • Positive control
    • WB: 293T, A431, H1299, HeLa, HepG2, Molt-4, Raji, Mouse brain lysates. IHC-P: NCIN87 xenograft tissue. ICC/IF: HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab154894 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 49 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)
ICC/IF 1/100 - 1/1000.

Target

  • Function
    This protein is a component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. Mediates pre-mRNA alternative splicing regulation. Inhibits, together with CUGBP1, insulin receptor (IR) pre-mRNA exon 11 inclusion in myoblast. Binds to the IR RNA. Binds poly(RG).
  • Tissue specificity
    Expressed ubiquitously.
  • Sequence similarities
    Contains 3 RRM (RNA recognition motif) domains.
  • Domain
    Each quasi-RRM repeat bound poly(RG), while only the N-terminal QRRM bound poly(RC) and poly(RU). None of the repeats bound detectable amounts of poly(RA).
  • Cellular localization
    Nucleus > nucleoplasm.
  • Information by UniProt
  • Database links
    see all
  • Alternative names
    • DKFZp686A15170 antibody
    • Heterogeneous nuclear ribonucleoprotein H antibody
    • Heterogeneous nuclear ribonucleoprotein H1 (H) antibody
    • Heterogeneous nuclear ribonucleoprotein H1 antibody
    • HNRH1_HUMAN antibody
    • hnRNP H antibody
    • hnRNPH antibody
    • Hnrnph1 antibody
    • HNRPH 1 antibody
    • HNRPH antibody
    • HNRPH1 protein antibody
    • N-terminally processed antibody
    see all

Images

  • Western blot - Anti-hnRNP H antibody (ab154894)
    Western blot - Anti-hnRNP H antibody (ab154894)
    All lanes : Anti-hnRNP H antibody (ab154894) at 1/1000 dilution

    Lane 1 : A431 whole cell lysate
    Lane 2 : H1299 whole cell lysate
    Lane 3 : HeLa whole cell lysate
    Lane 4 : HepG2 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 49 kDa



    10% SDS PAGE
  • Immunocytochemistry/ Immunofluorescence - Anti-hnRNP H antibody (ab154894)
    Immunocytochemistry/ Immunofluorescence - Anti-hnRNP H antibody (ab154894)
    Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, labeling hnRNP H with ab154894 at a 1/200 dilution (top image), and costained with Hoescht 33342 (bottom image)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP H antibody (ab154894)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP H antibody (ab154894)
    Immunohistochemical analysis of paraffin embedded Human NCIN87 xenograft tissue labeling hnRNP H with ab154894 at a 1/100 dilution.
  • Western blot - Anti-hnRNP H antibody (ab154894)
    Western blot - Anti-hnRNP H antibody (ab154894)
    Anti-hnRNP H antibody (ab154894) at 1/1000 dilution + Mouse brain whole cell lysate at 50 µg

    Predicted band size: 49 kDa



    10% SDS PAGE

References

ab154894 has not yet been referenced specifically in any publications.

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