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    hnrnp-h-antibody-ab154894.pdf

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Epigenetics and Nuclear Signaling DNA / RNA RNA Processing
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Anti-hnRNP H antibody (ab154894)

  • Datasheet
  • SDS
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Western blot - Anti-hnRNP H antibody (ab154894)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP H antibody (ab154894)
  • Immunocytochemistry/ Immunofluorescence - Anti-hnRNP H antibody (ab154894)
  • Western blot - Anti-hnRNP H antibody (ab154894)
  • Immunocytochemistry/ Immunofluorescence - Anti-hnRNP H antibody (ab154894)
  • Western blot - Anti-hnRNP H antibody (ab154894)

Key features and details

  • Rabbit polyclonal to hnRNP H
  • Suitable for: ICC/IF, WB, IHC-P
  • Reacts with: Mouse, Human
  • Isotype: IgG

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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

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Overview

  • Product name

    Anti-hnRNP H antibody
    See all hnRNP H primary antibodies
  • Description

    Rabbit polyclonal to hnRNP H
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Chicken, Cow, Xenopus laevis
  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 50-289 of Human hnRNP H (Uniprot ID P31943)

  • Positive control

    • WB: 293T, A431, H1299, HeLa, HepG2, Molt-4, Raji, Mouse brain lysates. Jurkat, Raji and NCI-H929 cells IHC-P: NCIN87 xenograft tissue. ICC/IF: HeLa and A431 cells
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • RNA Processing
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • RNA Processing
    • Other

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa whole cell lysate (ab29545)
    • Raji whole cell lysate (ab30124)
    • Mouse brain tissue lysate - total protein (ab30151)
    • A-431 whole cell lysate (ab7909)
    • MOLT-4 whole cell lysate (ab7912)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab154894 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
Use at an assay dependent concentration.
WB
1/500 - 1/3000. Predicted molecular weight: 49 kDa.
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)
Notes
ICC/IF
Use at an assay dependent concentration.
WB
1/500 - 1/3000. Predicted molecular weight: 49 kDa.
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)

Target

  • Function

    This protein is a component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. Mediates pre-mRNA alternative splicing regulation. Inhibits, together with CUGBP1, insulin receptor (IR) pre-mRNA exon 11 inclusion in myoblast. Binds to the IR RNA. Binds poly(RG).
  • Tissue specificity

    Expressed ubiquitously.
  • Sequence similarities

    Contains 3 RRM (RNA recognition motif) domains.
  • Domain

    Each quasi-RRM repeat bound poly(RG), while only the N-terminal QRRM bound poly(RC) and poly(RU). None of the repeats bound detectable amounts of poly(RA).
  • Cellular localization

    Nucleus > nucleoplasm.
  • Target information above from: UniProt accession P31943 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 395157 Chicken
    • Entrez Gene: 541202 Cow
    • Entrez Gene: 3187 Human
    • Entrez Gene: 59013 Mouse
    • Entrez Gene: 432071 Xenopus laevis
    • Entrez Gene: 446758 Xenopus laevis
    • Omim: 601035 Human
    • SwissProt: P31943 Human
    • SwissProt: O35737 Mouse
    • Unigene: 604001 Human
    • Unigene: 21740 Mouse
    see all
  • Alternative names

    • DKFZp686A15170 antibody
    • Heterogeneous nuclear ribonucleoprotein H antibody
    • Heterogeneous nuclear ribonucleoprotein H1 (H) antibody
    • Heterogeneous nuclear ribonucleoprotein H1 antibody
    • HNRH1_HUMAN antibody
    • hnRNP H antibody
    • hnRNPH antibody
    • Hnrnph1 antibody
    • HNRPH 1 antibody
    • HNRPH antibody
    • HNRPH1 protein antibody
    • N-terminally processed antibody
    see all

Images

  • Western blot - Anti-hnRNP H antibody (ab154894)
    Western blot - Anti-hnRNP H antibody (ab154894)
    All lanes : Anti-hnRNP H antibody (ab154894) at 1/1000 dilution

    Lane 1 : A431 whole cell lysate
    Lane 2 : H1299 whole cell lysate
    Lane 3 : HeLa whole cell lysate
    Lane 4 : HepG2 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 49 kDa



    10% SDS PAGE
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP H antibody (ab154894)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP H antibody (ab154894)
    Immunohistochemical analysis of paraffin embedded Human NCIN87 xenograft tissue labeling hnRNP H with ab154894 at a 1/100 dilution.
  • Immunocytochemistry/ Immunofluorescence - Anti-hnRNP H antibody (ab154894)
    Immunocytochemistry/ Immunofluorescence - Anti-hnRNP H antibody (ab154894)

    Immunocytochemistry/immunofluorescence analysis of A431 cells labelling hnRNP with ab154894 at 1/500 dilution (green). Cells were fixed with 4% paraformaldehyde. Cells were counterstained with alpha Tubulin - Microtubule Marker at 1/1000 dilution (red). 

  • Western blot - Anti-hnRNP H antibody (ab154894)
    Western blot - Anti-hnRNP H antibody (ab154894)
    All lanes : Anti-hnRNP H antibody (ab154894) at 1/1000 dilution

    Lane 1 : Jurkat cells
    Lane 2 : Raji cells
    Lane 3 : NCI-H2929 cells

    Predicted band size: 49 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-hnRNP H antibody (ab154894)
    Immunocytochemistry/ Immunofluorescence - Anti-hnRNP H antibody (ab154894)

    Immunocytochemistry/immunofluorescence analysis of HeLa cells labelling hnRNP with ab154894 at 1/200 dilution (green). Cells were fixed with 4% paraformaldehyde. Cells were counterstained with alpha Tubulin - Microtubule Marker at 1/10000 dilution (red). 

  • Western blot - Anti-hnRNP H antibody (ab154894)
    Western blot - Anti-hnRNP H antibody (ab154894)
    Anti-hnRNP H antibody (ab154894) at 1/1000 dilution + Mouse brain whole cell lysate at 50 µg

    Predicted band size: 49 kDa



    10% SDS PAGE

Protocols

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (4)

Publishing research using ab154894? Please let us know so that we can cite the reference in this datasheet.

ab154894 has been referenced in 4 publications.

  • Xu C  et al. HnRNP F/H associate with hTERC and telomerase holoenzyme to modulate telomerase function and promote cell proliferation. Cell Death Differ 27:1998-2013 (2020). PubMed: 31863069
  • Cozzolino F  et al. New label-free methods for protein relative quantification applied to the investigation of an animal model of Huntington Disease. PLoS One 15:e0238037 (2020). PubMed: 32886703
  • Koppers M  et al. Receptor-specific interactome as a hub for rapid cue-induced selective translation in axons. Elife 8:N/A (2019). PubMed: 31746735
  • Nikolaou KC  et al. The RNA-Binding Protein A1CF Regulates Hepatic Fructose and Glycerol Metabolism via Alternative RNA Splicing. Cell Rep 29:283-300.e8 (2019). PubMed: 31597092

Customer reviews and Q&As

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