Anti-hnRNP H antibody (ab154894)
Key features and details
- Rabbit polyclonal to hnRNP H
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-hnRNP H antibody
See all hnRNP H primary antibodies -
Description
Rabbit polyclonal to hnRNP H -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Chicken, Cow, Xenopus laevis
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Immunogen
Recombinant fragment corresponding to a region within amino acids 50-289 of Human hnRNP H (Uniprot ID P31943)
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Positive control
- WB: 293T, A431, H1299, HeLa, HepG2, Molt-4, Raji, Mouse brain lysates. Jurkat, Raji and NCI-H929 cells IHC-P: NCIN87 xenograft tissue. ICC/IF: HeLa and A431 cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab154894 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
Use at an assay dependent concentration.
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| WB |
1/500 - 1/3000. Predicted molecular weight: 49 kDa.
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| IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)
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| Notes |
|---|
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ICC/IF
Use at an assay dependent concentration. |
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WB
1/500 - 1/3000. Predicted molecular weight: 49 kDa. |
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IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0) |
Target
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Function
This protein is a component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. Mediates pre-mRNA alternative splicing regulation. Inhibits, together with CUGBP1, insulin receptor (IR) pre-mRNA exon 11 inclusion in myoblast. Binds to the IR RNA. Binds poly(RG). -
Tissue specificity
Expressed ubiquitously. -
Sequence similarities
Contains 3 RRM (RNA recognition motif) domains. -
Domain
Each quasi-RRM repeat bound poly(RG), while only the N-terminal QRRM bound poly(RC) and poly(RU). None of the repeats bound detectable amounts of poly(RA). -
Cellular localization
Nucleus > nucleoplasm. - Information by UniProt
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Database links
- Entrez Gene: 395157 Chicken
- Entrez Gene: 541202 Cow
- Entrez Gene: 3187 Human
- Entrez Gene: 59013 Mouse
- Entrez Gene: 432071 Xenopus laevis
- Entrez Gene: 446758 Xenopus laevis
- Omim: 601035 Human
- SwissProt: P31943 Human
see all -
Alternative names
- DKFZp686A15170 antibody
- Heterogeneous nuclear ribonucleoprotein H antibody
- Heterogeneous nuclear ribonucleoprotein H1 (H) antibody
see all
Images
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Western blot - Anti-hnRNP H antibody (ab154894)All lanes : Anti-hnRNP H antibody (ab154894) at 1/1000 dilution
Lane 1 : A431 whole cell lysate
Lane 2 : H1299 whole cell lysate
Lane 3 : HeLa whole cell lysate
Lane 4 : HepG2 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 49 kDa
10% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP H antibody (ab154894)Immunohistochemical analysis of paraffin embedded Human NCIN87 xenograft tissue labeling hnRNP H with ab154894 at a 1/100 dilution. -
Immunocytochemistry/ Immunofluorescence - Anti-hnRNP H antibody (ab154894)Immunocytochemistry/immunofluorescence analysis of A431 cells labelling hnRNP with ab154894 at 1/500 dilution (green). Cells were fixed with 4% paraformaldehyde. Cells were counterstained with alpha Tubulin - Microtubule Marker at 1/1000 dilution (red).
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Western blot - Anti-hnRNP H antibody (ab154894)All lanes : Anti-hnRNP H antibody (ab154894) at 1/1000 dilution
Lane 1 : Jurkat cells
Lane 2 : Raji cells
Lane 3 : NCI-H2929 cells
Predicted band size: 49 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-hnRNP H antibody (ab154894)Immunocytochemistry/immunofluorescence analysis of HeLa cells labelling hnRNP with ab154894 at 1/200 dilution (green). Cells were fixed with 4% paraformaldehyde. Cells were counterstained with alpha Tubulin - Microtubule Marker at 1/10000 dilution (red).
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Western blot - Anti-hnRNP H antibody (ab154894)Anti-hnRNP H antibody (ab154894) at 1/1000 dilution + Mouse brain whole cell lysate at 50 µg
Predicted band size: 49 kDa
10% SDS PAGE
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab154894 has been referenced in 4 publications.
- Xu C et al. HnRNP F/H associate with hTERC and telomerase holoenzyme to modulate telomerase function and promote cell proliferation. Cell Death Differ 27:1998-2013 (2020). PubMed: 31863069
- Cozzolino F et al. New label-free methods for protein relative quantification applied to the investigation of an animal model of Huntington Disease. PLoS One 15:e0238037 (2020). PubMed: 32886703
- Koppers M et al. Receptor-specific interactome as a hub for rapid cue-induced selective translation in axons. Elife 8:N/A (2019). PubMed: 31746735
- Nikolaou KC et al. The RNA-Binding Protein A1CF Regulates Hepatic Fructose and Glycerol Metabolism via Alternative RNA Splicing. Cell Rep 29:283-300.e8 (2019). PubMed: 31597092