Overview

  • Product name
    Anti-hnRNP K antibody [EPR10933]
    See all hnRNP K primary antibodies
  • Description
    Rabbit monoclonal [EPR10933] to hnRNP K
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human hnRNP K (P61978).

  • Positive control
    • HeLa and Jurkat cell lysates, Human glioma tissue
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR10933
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab156570 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 65 kDa (predicted molecular weight: 50 kDa).
IHC-P 1/50 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IP.
  • Target

    • Function
      One of the major pre-mRNA-binding proteins. Binds tenaciously to poly(C) sequences. Likely to play a role in the nuclear metabolism of hnRNAs, particularly for pre-mRNAs that contain cytidine-rich sequences. Can also bind poly(C) single-stranded DNA.
    • Sequence similarities
      Contains 3 KH domains.
    • Post-translational
      modifications
      Arg-296 and Arg-299 are dimethylated, probably to asymmetric dimethylarginine.
    • Cellular localization
      Cytoplasm. Nucleus > nucleoplasm. In case of ASFV infection, there is a shift in the localization which becomes predominantly nuclear.
    • Information by UniProt
    • Database links
    • Alternative names
      • CSBP antibody
      • dC stretch binding protein antibody
      • FLJ41122 antibody
      • Heterogeneous nuclear ribonucleoprotein K antibody
      • hnRNP K antibody
      • HNRNPK antibody
      • HNRPK antibody
      • HNRPK_HUMAN antibody
      • Transformation up regulated nuclear protein antibody
      • Transformation up-regulated nuclear protein antibody
      • Transformation upregulated nuclear protein antibody
      • TUNP antibody
      see all

    Images

    • ab156570 showing +ve staining in Human normal tonsil.
    • ab156570 showing +ve staining in Human thyroid gland carcinoma.
    • ab156570 showing +ve staining in Human normal pancreas.
    • ab156570 showing +ve staining in Human lung adenocarcinoma.
    • ab156570 showing +ve staining in Human urinary bladder carcinoma.
    • All lanes : Anti-hnRNP K antibody [EPR10933] (ab156570) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 50 kDa
      Observed band size: 65 kDa (why is the actual band size different from the predicted?)

    • Immunohistochemical analysis of paraffin embedded Human glioma tissue labeling hnRNP K with ab156570 antibody at 1/50.

    References

    This product has been referenced in:
    • Sawhney S  et al. Alpha-enolase is upregulated on the cell surface and responds to plasminogen activation in mice expressing a ?133p53a mimic. PLoS One 10:e0116270 (2015). WB ; Mouse . Read more (PubMed: 25643152) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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