Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR944] to hnRNP K
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Product nameAnti-hnRNP K antibody [EPR944]
See all hnRNP K primary antibodies
DescriptionRabbit monoclonal [EPR944] to hnRNP K
Tested applicationsSuitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human hnRNP K (internal sequence). The exact sequence is proprietary.
- Jurkat and A549 cell lysates; Human breast carcinoma tissue.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab134060 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/50000 - 1/200000. Predicted molecular weight: 50 kDa.|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionOne of the major pre-mRNA-binding proteins. Binds tenaciously to poly(C) sequences. Likely to play a role in the nuclear metabolism of hnRNAs, particularly for pre-mRNAs that contain cytidine-rich sequences. Can also bind poly(C) single-stranded DNA.
Sequence similaritiesContains 3 KH domains.
modificationsArg-296 and Arg-299 are dimethylated, probably to asymmetric dimethylarginine.
Cellular localizationCytoplasm. Nucleus > nucleoplasm. In case of ASFV infection, there is a shift in the localization which becomes predominantly nuclear.
- Information by UniProt
- CSBP antibody
- dC stretch binding protein antibody
- FLJ41122 antibody
All lanes : Anti-hnRNP K antibody [EPR944] (ab134060) at 1/50000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : A549 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 50 kDa
Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labelling hnRNP K with ab134060 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ab134060 has been referenced in 1 publication.
- Xiao X et al. Structural determinants of APOBEC3B non-catalytic domain for molecular assembly and catalytic regulation. Nucleic Acids Res 45:7494-7506 (2017). WB ; Human . PubMed: 28575276