Overview

  • Product name

    Anti-hnRNP K antibody [EPR944]
    See all hnRNP K primary antibodies
  • Description

    Rabbit monoclonal [EPR944] to hnRNP K
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human hnRNP K (internal sequence). The exact sequence is proprietary.

  • Positive control

    • Jurkat and A549 cell lysates; Human breast carcinoma tissue.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.40
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR944
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab134060 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50000 - 1/200000. Predicted molecular weight: 50 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IP.
  • Target

    • Function

      One of the major pre-mRNA-binding proteins. Binds tenaciously to poly(C) sequences. Likely to play a role in the nuclear metabolism of hnRNAs, particularly for pre-mRNAs that contain cytidine-rich sequences. Can also bind poly(C) single-stranded DNA.
    • Sequence similarities

      Contains 3 KH domains.
    • Post-translational
      modifications

      Arg-296 and Arg-299 are dimethylated, probably to asymmetric dimethylarginine.
    • Cellular localization

      Cytoplasm. Nucleus > nucleoplasm. In case of ASFV infection, there is a shift in the localization which becomes predominantly nuclear.
    • Information by UniProt
    • Database links

    • Alternative names

      • CSBP antibody
      • dC stretch binding protein antibody
      • FLJ41122 antibody
      • Heterogeneous nuclear ribonucleoprotein K antibody
      • hnRNP K antibody
      • HNRNPK antibody
      • HNRPK antibody
      • HNRPK_HUMAN antibody
      • Transformation up regulated nuclear protein antibody
      • Transformation up-regulated nuclear protein antibody
      • Transformation upregulated nuclear protein antibody
      • TUNP antibody
      see all

    Images

    • All lanes : Anti-hnRNP K antibody [EPR944] (ab134060) at 1/50000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : A549 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 50 kDa

    • Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labelling hnRNP K with ab134060 at 1/100 dilution.

    References

    This product has been referenced in:

    • Xiao X  et al. Structural determinants of APOBEC3B non-catalytic domain for molecular assembly and catalytic regulation. Nucleic Acids Res 45:7494-7506 (2017). WB ; Human . Read more (PubMed: 28575276) »
    See 1 Publication for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab134060.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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