Anti-hnRNP K antibody [F45 P9 C7] (ab23644)

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Western blot - Anti-hnRNP K antibody [F45 P9 C7] (ab23644)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP K antibody [F45 P9 C7] (ab23644)
  • Immunocytochemistry/ Immunofluorescence - Anti-hnRNP K antibody [F45 P9 C7] (ab23644)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP K antibody [F45 P9 C7] (ab23644)
  • Western blot - Anti-hnRNP K antibody [F45 P9 C7] (ab23644)
  • Flow Cytometry - Anti-hnRNP K antibody [F45 P9 C7] (ab23644)
  • Immunocytochemistry/ Immunofluorescence - Anti-hnRNP K antibody [F45 P9 C7] (ab23644)

Key features and details

  • Mouse monoclonal [F45 P9 C7] to hnRNP K
  • Suitable for: ICC/IF, Flow Cyt, WB, IHC-P
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-hnRNP K antibody [F45 P9 C7]
    See all hnRNP K primary antibodies

  • Description

    Mouse monoclonal [F45 P9 C7] to hnRNP K

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide corresponding to Human hnRNP K aa 450 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab32584)

  • Positive control

    • This antibody gave a positive signal in the following lysates: NIH 3T3 whole cell, MEF1 whole cell; PC12 whole cell; mouse testis tissue; mouse lung tissue.

  • General notes

    This antibody clone is manufactured by Abcam.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Some batches contain 6.97% L-Arginine as a stabilizing agent. For lot-specific buffer information, please contact our Scientific Support team.
  • Concentration information loading...

  • Purity

    IgG fraction

  • Clonality

    Monoclonal

  • Clone number

    F45 P9 C7

  • Myeloma

    Ag8.653

  • Isotype

    IgG1

  • Light chain type

    kappa

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab23644 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
Flow Cyt
Human
ICC/IF
Human
IHC-P
Human
WB
Mouse
Human
All applications
Rabbit
Chicken
Application Abreviews Notes
ICC/IF
Use a concentration of 5 µg/ml.
Flow Cyt
Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 64 kDa (predicted molecular weight: 51 kDa).
IHC-P (1)
Use at an assay dependent concentration. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
Notes
ICC/IF
Use a concentration of 5 µg/ml.
Flow Cyt
Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 64 kDa (predicted molecular weight: 51 kDa).
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.

Target

  • Function

    One of the major pre-mRNA-binding proteins. Binds tenaciously to poly(C) sequences. Likely to play a role in the nuclear metabolism of hnRNAs, particularly for pre-mRNAs that contain cytidine-rich sequences. Can also bind poly(C) single-stranded DNA.

  • Sequence similarities

    Contains 3 KH domains.

  • Post-translational
    modifications

    Arg-296 and Arg-299 are dimethylated, probably to asymmetric dimethylarginine.

  • Cellular localization

    Cytoplasm. Nucleus > nucleoplasm. In case of ASFV infection, there is a shift in the localization which becomes predominantly nuclear.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • CSBP antibody
    • dC stretch binding protein antibody
    • FLJ41122 antibody
    • Heterogeneous nuclear ribonucleoprotein K antibody
    • hnRNP K antibody
    • HNRNPK antibody
    • HNRPK antibody
    • HNRPK_HUMAN antibody
    • Transformation up regulated nuclear protein antibody
    • Transformation up-regulated nuclear protein antibody
    • Transformation upregulated nuclear protein antibody
    • TUNP antibody
    see all

Images

  • Western blot - Anti-hnRNP K antibody [F45 P9 C7] (ab23644)
    Western blot - Anti-hnRNP K antibody [F45 P9 C7] (ab23644)
    Lane 1 : Marker
    Lanes 2-5 : Anti-hnRNP K antibody [F45 P9 C7] (ab23644) at 1 µg/ml

    Lane 2 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate (ab27251) at 20 µg
    Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate (ab27252) at 20 µg
    Lane 4 : Jurkat whole cell lysate (ab7899) at 20 µg
    Lane 5 : HEK-293 whole cell lysate (ab7902) at 20 µg

    Secondary
    Lanes 2-5 : Rabbit Anti-Mouse IgG H&L (HRP) (ab6728) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 51 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP K antibody [F45 P9 C7] (ab23644)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP K antibody [F45 P9 C7] (ab23644)
    Formalin-fixed, paraffin embedded tissue sections from human normal colon or human colon carcinoma stained with ab23644 mouse monoclonal to hnRNP K. hnRNP K expression is increased in the tumour tissue.
  • Immunocytochemistry/ Immunofluorescence - Anti-hnRNP K antibody [F45 P9 C7] (ab23644)
    Immunocytochemistry/ Immunofluorescence - Anti-hnRNP K antibody [F45 P9 C7] (ab23644)

    ICC/IF image of ab23644 stained human HepG2 cells. The cells were 4% PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab23644, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).  This antibody also gave a positive IF result in HeLa, HEK 293 and MCF7 cells.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP K antibody [F45 P9 C7] (ab23644)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP K antibody [F45 P9 C7] (ab23644)This image is courtesy of an Abreview submitted by Satyendra Tripathi
    ab23644 staining hnRNP K in Human head and neck tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA for 1 hour at 25°C; antigen retrieval was by heat mediation with Tris EDTA (pH9). Samples were incubated with primary antibody (1 µg/ml in TBST) for 16 hours at 4°C. An undiluted HRP-conjugated Rabbit anti-mouse polyclonal was used as the secondary antibody.

    See Abreview

  • Western blot - Anti-hnRNP K antibody [F45 P9 C7] (ab23644)
    Western blot - Anti-hnRNP K antibody [F45 P9 C7] (ab23644)
    All lanes : Anti-hnRNP K antibody [F45 P9 C7] (ab23644) at 5 µg/ml

    Lane 1 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
    Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
    Lane 3 : Testis (Mouse) Tissue Lysate
    Lane 4 : Lung (Mouse) Tissue Lysate
    Lane 5 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 51 kDa
    Observed band size: 56 kDa why is the actual band size different from the predicted?
    Additional bands at: 21 kDa, 24 kDa, 54 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 4 minutes
  • Flow Cytometry - Anti-hnRNP K antibody [F45 P9 C7] (ab23644)
    Flow Cytometry - Anti-hnRNP K antibody [F45 P9 C7] (ab23644)
    Overlay histogram showing HeLa cells stained with ab23644 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab23644, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

  • Immunocytochemistry/ Immunofluorescence - Anti-hnRNP K antibody [F45 P9 C7] (ab23644)
    Immunocytochemistry/ Immunofluorescence - Anti-hnRNP K antibody [F45 P9 C7] (ab23644)Image from Meyerowitz J et al., Mol Neurodegener. 2011 Aug 8;6:57. Fig 10.; doi:10.1186/1750-1326-6-57; 8 August 2011, Molecular Neurodegeneration 2011, 6:57
    Immunofluorescence analysis of SH-SY5Y cells staining hnRNP K with ab23644.

    Cells were fixed with paraformaldehyde in PBS for 30 min and permeabilized with 90% chilled methanol for 5 min. After blocking for 1 hour with 10% normal goat serum, cells were incubated with primary antibody (1/200) overnight at 4°C. An AlexaFluor®-conjugated goat anti-mouse IgG (1/500) was used as the secondary antibody.

References (10)

Publishing research using ab23644? Please let us know so that we can cite the reference in this datasheet.

ab23644 has been referenced in 10 publications.

  • Zhao S  et al. hnRNP K plays a protective role in TNF-a-induced apoptosis in podocytes. Biosci Rep 38:N/A (2018). WB . PubMed: 29724888
  • Pellegrini L  et al. Proteomic analysis reveals co-ordinated alterations in protein synthesis and degradation pathways in LRRK2 knockout mice. Hum Mol Genet 27:3257-3271 (2018). PubMed: 29917075
  • Hoch-Kraft P  et al. Dual role of the RNA helicase DDX5 in post-transcriptional regulation of myelin basic protein in oligodendrocytes. J Cell Sci 131:N/A (2018). PubMed: 29622601
  • Kumar M  et al. Nuclear heterogeneous nuclear ribonucleoprotein D is associated with poor prognosis and interactome analysis reveals its novel binding partners in oral cancer. J Transl Med 13:285 (2015). PubMed: 26318153
  • Chauhan SS  et al. Prediction of recurrence-free survival using a protein expression-based risk classifier for head and neck cancer. Oncogenesis 4:e147 (2015). IHC . PubMed: 25893634
  • Kaur J  et al. S100A7 overexpression is a predictive marker for high risk of malignant transformation in oral dysplasia. Int J Cancer 134:1379-88 (2014). PubMed: 24122701
  • Satkunanathan S  et al. Establishment of a novel cell line for the enhanced production of recombinant adeno-associated virus vectors for gene therapy. Hum Gene Ther 25:929-41 (2014). WB . PubMed: 25072415
  • van Domselaar R  et al. All Human Granzymes Target hnRNP K That Is Essential for Tumor Cell Viability. J Biol Chem 287:22854-64 (2012). WB . PubMed: 22582387
  • Meyerowitz J  et al. C-Jun N-terminal kinase controls TDP-43 accumulation in stress granules induced by oxidative stress. Mol Neurodegener 6:57 (2011). ICC/IF ; Human . PubMed: 21819629
  • Matta A  et al. Heterogeneous ribonucleoprotein K is a marker of oral leukoplakia and correlates with poor prognosis of squamous cell carcinoma. Int J Cancer 125:1398-406 (2009). IHC-P ; Human . PubMed: 19548310

Customer reviews and Q&As

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1-2 of 2 Abreviews or Q&A

Immunohistochemistry (Frozen sections) abreview for Anti-hnRNP K antibody [F45 P9 C7]

 Good
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Brain Frontal Cortex)
Specification
Brain Frontal Cortex
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: RT°C
Fixative
Acetone
Read More

Abcam user community

Verified customer

Submitted Jan 21 2020

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-hnRNP K antibody [F45 P9 C7]

 Excellent
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Head and neck)
Specification
Head and neck
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris EDTA pH 9.0
Permeabilization
No
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Read More

Dr. Satyendra Tripathi

Verified customer

Submitted Sep 14 2010

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