Overview

  • Product name

    Anti-hnRNP K antibody [F45 P9 C7] (Alexa Fluor® 647)
    See all hnRNP K primary antibodies
  • Description

    Mouse monoclonal [F45 P9 C7] to hnRNP K (Alexa Fluor® 647)
  • Host species

    Mouse
  • Conjugation

    Alexa Fluor® 647. Ex: 652nm, Em: 668nm
  • Tested applications

    Suitable for: ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human hnRNP K aa 450 to the C-terminus conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    Database link: P61978
    (Peptide available as ab32584)

  • Positive control

    • ICC/IF: HeLa cells

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark.
  • Storage buffer

    pH: 7.4
    Preservative: 0.02% Sodium azide
    Constituents: 30% Glycerol, 1% BSA, PBS
  • Concentration information loading...
  • Purity

    IgG fraction
  • Clonality

    Monoclonal
  • Clone number

    F45 P9 C7
  • Myeloma

    Ag8.653
  • Isotype

    IgG1
  • Light chain type

    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab204455 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100.

This product gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min).

Target

  • Function

    One of the major pre-mRNA-binding proteins. Binds tenaciously to poly(C) sequences. Likely to play a role in the nuclear metabolism of hnRNAs, particularly for pre-mRNAs that contain cytidine-rich sequences. Can also bind poly(C) single-stranded DNA.
  • Sequence similarities

    Contains 3 KH domains.
  • Post-translational
    modifications

    Arg-296 and Arg-299 are dimethylated, probably to asymmetric dimethylarginine.
  • Cellular localization

    Cytoplasm. Nucleus > nucleoplasm. In case of ASFV infection, there is a shift in the localization which becomes predominantly nuclear.
  • Information by UniProt
  • Database links

  • Alternative names

    • CSBP antibody
    • dC stretch binding protein antibody
    • FLJ41122 antibody
    • Heterogeneous nuclear ribonucleoprotein K antibody
    • hnRNP K antibody
    • HNRNPK antibody
    • HNRPK antibody
    • HNRPK_HUMAN antibody
    • Transformation up regulated nuclear protein antibody
    • Transformation up-regulated nuclear protein antibody
    • Transformation upregulated nuclear protein antibody
    • TUNP antibody
    see all

Images

  • ab204455 staining hnRNP K in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab204455 at a 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at a 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 4% formaldehyde (10 min).

References

ab204455 has not yet been referenced specifically in any publications.

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