Key features and details
- Rabbit polyclonal to hnRNP K (phospho S284)
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-hnRNP K (phospho S284) antibody
See all hnRNP K primary antibodies
DescriptionRabbit polyclonal to hnRNP K (phospho S284)
Specificityab74066 detects endogenous levels of hnRNP K only when phosphorylated at serine 284.
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Human
Synthetic phosphopeptide derived from human hnRNP K around the phosphorylation site of serine 284 (D-M-SP-P-R).
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab74066 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Our Abpromise guarantee covers the use of ab74066 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa).|
FunctionOne of the major pre-mRNA-binding proteins. Binds tenaciously to poly(C) sequences. Likely to play a role in the nuclear metabolism of hnRNAs, particularly for pre-mRNAs that contain cytidine-rich sequences. Can also bind poly(C) single-stranded DNA.
Sequence similaritiesContains 3 KH domains.
modificationsArg-296 and Arg-299 are dimethylated, probably to asymmetric dimethylarginine.
Cellular localizationCytoplasm. Nucleus > nucleoplasm. In case of ASFV infection, there is a shift in the localization which becomes predominantly nuclear.
- Information by UniProt
- CSBP antibody
- dC stretch binding protein antibody
- FLJ41122 antibody
All lanes : Anti-hnRNP K (phospho S284) antibody (ab74066) at 1/500 dilution
Lane 1 : Extracts from 3T3 cells treated
with EGF (200ng/ml, 30mins)
Lane 2 : Extracts from 3T3 cells treated
with EGF (200ng/ml, 30mins) with immunising phosphopeptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 51 kDa
Observed band size: 51 kDa
ab74066 has been referenced in 1 publication.
- Zhao L et al. The anticancer effects of cinobufagin on hepatocellular carcinoma Huh-7 cells are associated with activation of the p73 signaling pathway. Mol Med Rep 19:4119-4128 (2019). PubMed: 30942456