Overview

  • Product name

    Anti-hnRNP K (phospho S284) antibody
    See all hnRNP K primary antibodies
  • Description

    Rabbit polyclonal to hnRNP K (phospho S284)
  • Host species

    Rabbit
  • Specificity

    ab74066 detects endogenous levels of hnRNP K only when phosphorylated at serine 284.
  • Tested applications

    Suitable for: WB, ELISAmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic phosphopeptide derived from human hnRNP K around the phosphorylation site of serine 284 (D-M-SP-P-R).

  • Positive control

    • Extracts from 3T3 cells treated with EGF (200ng/ml, 30mins).

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS

    Without Mg2+ and Ca2+
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab74066 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab74066 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa).
ELISA 1/20000.

Target

  • Function

    One of the major pre-mRNA-binding proteins. Binds tenaciously to poly(C) sequences. Likely to play a role in the nuclear metabolism of hnRNAs, particularly for pre-mRNAs that contain cytidine-rich sequences. Can also bind poly(C) single-stranded DNA.
  • Sequence similarities

    Contains 3 KH domains.
  • Post-translational
    modifications

    Arg-296 and Arg-299 are dimethylated, probably to asymmetric dimethylarginine.
  • Cellular localization

    Cytoplasm. Nucleus > nucleoplasm. In case of ASFV infection, there is a shift in the localization which becomes predominantly nuclear.
  • Information by UniProt
  • Database links

  • Alternative names

    • CSBP antibody
    • dC stretch binding protein antibody
    • FLJ41122 antibody
    • Heterogeneous nuclear ribonucleoprotein K antibody
    • hnRNP K antibody
    • HNRNPK antibody
    • HNRPK antibody
    • HNRPK_HUMAN antibody
    • Transformation up regulated nuclear protein antibody
    • Transformation up-regulated nuclear protein antibody
    • Transformation upregulated nuclear protein antibody
    • TUNP antibody
    see all

Images

  • All lanes : Anti-hnRNP K (phospho S284) antibody (ab74066) at 1/500 dilution

    Lane 1 : Extracts from 3T3 cells treated
    with EGF (200ng/ml, 30mins)
    Lane 2 : Extracts from 3T3 cells treated
    with EGF (200ng/ml, 30mins) with immunising phosphopeptide at 10 µg

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 51 kDa
    Observed band size: 51 kDa

References

ab74066 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

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Unfortunately the blocking peptide for this antibody is not available.

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