Product nameAnti-hnRNP L antibody [4D11]
See all hnRNP L primary antibodies
DescriptionMouse monoclonal [4D11] to hnRNP L
Tested applicationsSuitable for: IHC-Fr, IHC-P, ELISA, WB, IP, Flow Cyt, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human, Newt
Human hnRNP proteins (from HeLa cells) purified by affinity chromatography on ssDNA agarose.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.1% Sodium azide
Concentration information loading...
PurityProtein A purified
Purification notesPurified from supernatant.
Our Abpromise guarantee covers the use of ab6106 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use at an assay dependent concentration.|
|IHC-P||Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ELISA||Use at an assay dependent concentration.|
|WB||1/2000. Detects a band of approximately 64 kDa (predicted molecular weight: 64 kDa).|
|IP||Use at an assay dependent concentration.|
|Flow Cyt||Use 1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|ICC/IF||Use at an assay dependent concentration.|
FunctionThis protein is a component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. Is associated with most nascent transcripts including those of the landmark giant loops of amphibian lampbrush chromosomes. Associates, together with APEX1, to the negative calcium responsive element (nCaRE) B2 of the APEX2 promoter.
Sequence similaritiesContains 3 RRM (RNA recognition motif) domains.
modificationsSeveral isoelectric forms of the L protein are probably the results of post-translational modifications.
Cellular localizationNucleus > nucleoplasm. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
- Information by UniProt
- D830027H13Rik antibody
- FLJ35509 antibody
- Heterogeneous nuclear ribonucleoprotein L antibody
All lanes : Anti-hnRNP L antibody [4D11] (ab6106) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 3 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 64 kDa
Observed band size: 64 kDa
Additional bands at: 60 kDa, 68 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 1 minute
IHC image of ab6106 staining in human normal skin formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab6106, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
hnRNP L was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Mouse monoclonal to hnRNP L (ab6106) and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab6106.
Secondary: Goat polyclonal to mouse IgG light chain specific (HRP) at 1/5000 dilution.
Band: 65kDa: hnRNP L. Non specific - 70kDa and 55kDa: We are unsure as to the identity of this extra band.
Overlay histogram showing Jurkat cells stained with ab6106 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab6106, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Immunofluorescence analysis of Human corneal endothelial cells, staining hnRNP L with ab6106.
Cells were fixed with formaldehyde, permeabilized with 1% Triton X-100 and blocked with serum for 30 minutes at 37°C. Samples were incubated with primary antibody (1/200 in 2% BSA + 2% goat serum) for 1 hour at 37°C. An AlexaFluor®488-conjugated goat anti-mouse polyclonal IgG (1/500) was used as the secondary antibody.
This product has been referenced in:
- Majewski L et al. Myosin VI in the nucleus of neurosecretory PC12 cells: Stimulation-dependent nuclear translocation and interaction with nuclear proteins. Nucleus 9:125-141 (2018). Read more (PubMed: 29293066) »
- Orecchini E et al. ADAR1 restricts LINE-1 retrotransposition. Nucleic Acids Res 45:155-168 (2017). Read more (PubMed: 27658966) »