Overview

  • Product name

  • Description

    Rabbit polyclonal to hnRNP L
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide corresponding to amino acid residues from an internal region of Human hnRNP L (NP001524.2).

  • Positive control

    • Human testis tissue; Human small intestine tissue; HeLa cell lysate; Jurkat cell lysate; HuvEc cell lysate; HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab139381 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 64 kDa.
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/500.

Target

  • Function

    This protein is a component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. Is associated with most nascent transcripts including those of the landmark giant loops of amphibian lampbrush chromosomes. Associates, together with APEX1, to the negative calcium responsive element (nCaRE) B2 of the APEX2 promoter.
  • Sequence similarities

    Contains 3 RRM (RNA recognition motif) domains.
  • Post-translational
    modifications

    Several isoelectric forms of the L protein are probably the results of post-translational modifications.
  • Cellular localization

    Nucleus > nucleoplasm. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
  • Information by UniProt
  • Database links

  • Alternative names

    • D830027H13Rik antibody
    • FLJ35509 antibody
    • Heterogeneous nuclear ribonucleoprotein L antibody
    • hnRNP L antibody
    • hnRNP-L antibody
    • Hnrnpl antibody
    • hnRPL antibody
    • HNRPL_HUMAN antibody
    • P/OKcl.14 antibody
    see all

Images

  • All lanes : Anti-hnRNP L antibody (ab139381) at 1/500 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : HuvEc cell lysate
    Lane 4 : Treated with the Synthesized peptide

    Predicted band size: 64 kDa

  • Immunohistochemistry of formalin-fixed, paraffin-embedded Human testis tissue labelling hnRNP L with ab139381 at 10µg/ml.
  • Immunohistochemistry of formalin-fixed, paraffin-embedded Human small intestine tissue labelling hnRNP L with ab139381 at 10µg/ml.
  • Immunofluorescence analysis of HeLa cells labelling hnRNP L with ab139381 at 1/100 dilution (left) and synthetized peptide (right).

References

ab139381 has not yet been referenced specifically in any publications.

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