Key features and details
- Rabbit polyclonal to hnRNP L
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-hnRNP L antibody
See all hnRNP L primary antibodies
DescriptionRabbit polyclonal to hnRNP L
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
Synthetic peptide corresponding to amino acid residues from an internal region of Human hnRNP L (NP001524.2).
- Human testis tissue; Human small intestine tissue; HeLa cell lysate; Jurkat cell lysate; HuvEc cell lysate; HeLa cells.
Storage instructionsShipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Store undiluted.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 0.88% Sodium chloride, 50% Glycerol, 49% PBS
PBS without Ca2+ and Mg2+
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab139381 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 64 kDa.|
|IHC-P||Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|ICC/IF||1/100 - 1/500.|
FunctionThis protein is a component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. Is associated with most nascent transcripts including those of the landmark giant loops of amphibian lampbrush chromosomes. Associates, together with APEX1, to the negative calcium responsive element (nCaRE) B2 of the APEX2 promoter.
Sequence similaritiesContains 3 RRM (RNA recognition motif) domains.
modificationsSeveral isoelectric forms of the L protein are probably the results of post-translational modifications.
Cellular localizationNucleus > nucleoplasm. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
- Information by UniProt
- D830027H13Rik antibody
- FLJ35509 antibody
- Heterogeneous nuclear ribonucleoprotein L antibody
All lanes : Anti-hnRNP L antibody (ab139381) at 1/500 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : HuvEc cell lysate
Lane 4 : Treated with the Synthesized peptide
Predicted band size: 64 kDa
Immunohistochemistry of formalin-fixed, paraffin-embedded Human testis tissue labelling hnRNP L with ab139381 at 10µg/ml.
Immunohistochemistry of formalin-fixed, paraffin-embedded Human small intestine tissue labelling hnRNP L with ab139381 at 10µg/ml.
Immunofluorescence analysis of HeLa cells labelling hnRNP L with ab139381 at 1/100 dilution (left) and synthetized peptide (right).
ab139381 has not yet been referenced specifically in any publications.