Anti-hnRNP L antibody (ab156682)
Key features and details
- Rabbit polyclonal to hnRNP L
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-hnRNP L antibody
See all hnRNP L primary antibodies -
Description
Rabbit polyclonal to hnRNP L -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Chimpanzee, Ferret, Non human primates, Rhesus monkey, Gorilla, Orangutan
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Immunogen
Synthetic peptide corresponding to a region within amino acids 350-400 of Human hnRNP L (NP_001524.2).
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Positive control
- 293T, HeLa and Jurkat whole cell lysates; mouse NIH 3T3 whole cell lysates.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab156682 was affinity purified using an epitope specific to hnRNP L immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab156682 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/2000 - 1/10000. Predicted molecular weight: 64 kDa. | |
| IP | Use at 2-10 µg/mg of lysate. |
Target
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Function
This protein is a component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. Is associated with most nascent transcripts including those of the landmark giant loops of amphibian lampbrush chromosomes. Associates, together with APEX1, to the negative calcium responsive element (nCaRE) B2 of the APEX2 promoter. -
Sequence similarities
Contains 3 RRM (RNA recognition motif) domains. -
Post-translational
modificationsSeveral isoelectric forms of the L protein are probably the results of post-translational modifications. -
Cellular localization
Nucleus > nucleoplasm. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. - Information by UniProt
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Database links
- Entrez Gene: 504520 Cow
- Entrez Gene: 476466 Dog
- Entrez Gene: 100734238 Guinea pig
- Entrez Gene: 100066483 Horse
- Entrez Gene: 3191 Human
- Entrez Gene: 15388 Mouse
- Entrez Gene: 80846 Rat
- Omim: 603083 Human
see all -
Alternative names
- D830027H13Rik antibody
- FLJ35509 antibody
- Heterogeneous nuclear ribonucleoprotein L antibody
see all
Images
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Western blot - Anti-hnRNP L antibody (ab156682)All lanes : Anti-hnRNP L antibody (ab156682) at 0.1 µg/ml
Lane 1 : 293T whole cell lysate at 50 µg
Lane 2 : 293T whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 64 kDa
Exposure time: 30 seconds -
Western blot - Anti-hnRNP L antibody (ab156682)All lanes : Anti-hnRNP L antibody (ab156682) at 0.4 µg/ml
Lane 1 : Mouse NIH 3T3 whole cell lysate at 50 µg
Lane 2 : Mouse NIH 3T3 whole cell lysate at 15 µg
Developed using the ECL technique.
Predicted band size: 64 kDa
Exposure time: 10 seconds -
Immunoprecipitation - Anti-hnRNP L antibody (ab156682)Detection of hnRNP L by Western blot of Immunoprecipitate
Lane 1: Immunoprecipitation of 293T whole cell lysate using ab156682 at 6 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Lane 2: lysate is precipitated with an antibody to another region of hnRNP L.
Lane 3: lysate is precipitated using an IgG control antibody.
Detection: using ab156682 at 1 µg/ml.
Protocols
Datasheets and documents
References (1)
ab156682 has been referenced in 1 publication.
- Cera I et al. Genes encoding SATB2-interacting proteins in adult cerebral cortex contribute to human cognitive ability. PLoS Genet 15:e1007890 (2019). PubMed: 30726206
