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- Specific References
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Overview
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Product nameAnti-hnRNP L antibody
See all hnRNP L primary antibodies -
DescriptionRabbit polyclonal to hnRNP L
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Host speciesRabbit
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Tested applicationsSuitable for: WB, IPmore details
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Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Chimpanzee, Ferret, Non human primates, Rhesus monkey, Gorilla, Orangutan
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Immunogen
Synthetic peptide corresponding to a region within amino acids 350-400 of Human hnRNP L (NP_001524.2).
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Positive control
- 293T, HeLa and Jurkat whole cell lysates; mouse NIH 3T3 whole cell lysates.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C.
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Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
Note: pH 7 to 8 -
Concentration information loading... -
PurityImmunogen affinity purified
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Purification notesab156682 was affinity purified using an epitope specific to hnRNP L immobilized on solid support.
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab156682 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/2000 - 1/10000. Predicted molecular weight: 64 kDa. | |
| IP | Use at 2-10 µg/mg of lysate. |
Target
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FunctionThis protein is a component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. Is associated with most nascent transcripts including those of the landmark giant loops of amphibian lampbrush chromosomes. Associates, together with APEX1, to the negative calcium responsive element (nCaRE) B2 of the APEX2 promoter.
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Sequence similaritiesContains 3 RRM (RNA recognition motif) domains.
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Post-translational
modificationsSeveral isoelectric forms of the L protein are probably the results of post-translational modifications. -
Cellular localizationNucleus > nucleoplasm. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
- Information by UniProt
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Database links
- Entrez Gene: 504520 Cow
- Entrez Gene: 476466 Dog
- Entrez Gene: 100734238 Guinea pig
- Entrez Gene: 100066483 Horse
- Entrez Gene: 3191 Human
- Entrez Gene: 15388 Mouse
- Entrez Gene: 80846 Rat
- Omim: 603083 Human
see all -
Alternative names
- D830027H13Rik antibody
- FLJ35509 antibody
- Heterogeneous nuclear ribonucleoprotein L antibody
see all
Images
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Western blot - Anti-hnRNP L antibody (ab156682)All lanes : Anti-hnRNP L antibody (ab156682) at 0.1 µg/ml
Lane 1 : 293T whole cell lysate at 50 µg
Lane 2 : 293T whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 64 kDa
Exposure time: 30 seconds -
Western blot - Anti-hnRNP L antibody (ab156682)All lanes : Anti-hnRNP L antibody (ab156682) at 0.4 µg/ml
Lane 1 : Mouse NIH 3T3 whole cell lysate at 50 µg
Lane 2 : Mouse NIH 3T3 whole cell lysate at 15 µg
Developed using the ECL technique.
Predicted band size: 64 kDa
Exposure time: 10 seconds -
Immunoprecipitation - Anti-hnRNP L antibody (ab156682)Detection of hnRNP L by Western blot of Immunoprecipitate
Lane 1: Immunoprecipitation of 293T whole cell lysate using ab156682 at 6 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Lane 2: lysate is precipitated with an antibody to another region of hnRNP L.
Lane 3: lysate is precipitated using an IgG control antibody.
Detection: using ab156682 at 1 µg/ml.
References
ab156682 has not yet been referenced specifically in any publications.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"