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    hnrnp-l-antibody-ab156682.pdf

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Tags & Cell Markers Subcellular Markers Nucleus Nucleoplasm
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Anti-hnRNP L antibody (ab156682)

  • Datasheet
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Western blot - Anti-hnRNP L antibody (ab156682)
  • Western blot - Anti-hnRNP L antibody (ab156682)
  • Immunoprecipitation - Anti-hnRNP L antibody (ab156682)

Key features and details

  • Rabbit polyclonal to hnRNP L
  • Suitable for: WB, IP
  • Reacts with: Mouse, Human
  • Isotype: IgG

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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

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Overview

  • Product name

    Anti-hnRNP L antibody
    See all hnRNP L primary antibodies
  • Description

    Rabbit polyclonal to hnRNP L
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Chimpanzee, Ferret, Non human primates, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to a region within amino acids 350-400 of Human hnRNP L (NP_001524.2).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • 293T, HeLa and Jurkat whole cell lysates; mouse NIH 3T3 whole cell lysates.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    pH: 7
    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate

    pH 7 to 8
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab156682 was affinity purified using an epitope specific to hnRNP L immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Subcellular Markers
    • Nucleus
    • Nucleoplasm
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • RNA Processing
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • RNA Processing
    • Other

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • HeLa whole cell lysate (ab29545)
    • NIH 3T3 whole cell lysate (ab7179)
    • Jurkat whole cell lysate (ab7899)

Applications

Our Abpromise guarantee covers the use of ab156682 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 64 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function

    This protein is a component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. Is associated with most nascent transcripts including those of the landmark giant loops of amphibian lampbrush chromosomes. Associates, together with APEX1, to the negative calcium responsive element (nCaRE) B2 of the APEX2 promoter.
  • Sequence similarities

    Contains 3 RRM (RNA recognition motif) domains.
  • Post-translational
    modifications

    Several isoelectric forms of the L protein are probably the results of post-translational modifications.
  • Cellular localization

    Nucleus > nucleoplasm. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
  • Target information above from: UniProt accession P14866 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 504520 Cow
    • Entrez Gene: 476466 Dog
    • Entrez Gene: 100734238 Guinea pig
    • Entrez Gene: 100066483 Horse
    • Entrez Gene: 3191 Human
    • Entrez Gene: 15388 Mouse
    • Entrez Gene: 80846 Rat
    • Omim: 603083 Human
    • SwissProt: P14866 Human
    • SwissProt: Q8R081 Mouse
    • Unigene: 446623 Human
    • Unigene: 644906 Human
    • Unigene: 9043 Mouse
    • Unigene: 33873 Rat
    see all
  • Alternative names

    • D830027H13Rik antibody
    • FLJ35509 antibody
    • Heterogeneous nuclear ribonucleoprotein L antibody
    • hnRNP L antibody
    • hnRNP-L antibody
    • Hnrnpl antibody
    • hnRPL antibody
    • HNRPL_HUMAN antibody
    • P/OKcl.14 antibody
    see all

Images

  • Western blot - Anti-hnRNP L antibody (ab156682)
    Western blot - Anti-hnRNP L antibody (ab156682)
    All lanes : Anti-hnRNP L antibody (ab156682) at 0.1 µg/ml

    Lane 1 : 293T whole cell lysate at 50 µg
    Lane 2 : 293T whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 50 µg
    Lane 4 : Jurkat whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 64 kDa


    Exposure time: 30 seconds
  • Western blot - Anti-hnRNP L antibody (ab156682)
    Western blot - Anti-hnRNP L antibody (ab156682)
    All lanes : Anti-hnRNP L antibody (ab156682) at 0.4 µg/ml

    Lane 1 : Mouse NIH 3T3 whole cell lysate at 50 µg
    Lane 2 : Mouse NIH 3T3 whole cell lysate at 15 µg

    Developed using the ECL technique.

    Predicted band size: 64 kDa


    Exposure time: 10 seconds
  • Immunoprecipitation - Anti-hnRNP L antibody (ab156682)
    Immunoprecipitation - Anti-hnRNP L antibody (ab156682)
    Detection of hnRNP L by Western blot of Immunoprecipitate
    Lane 1: Immunoprecipitation of 293T whole cell lysate using ab156682 at 6 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Lane 2: lysate is precipitated with an antibody to another region of hnRNP L.
    Lane 3: lysate is precipitated using an IgG control antibody.
    Detection: using ab156682 at 1 µg/ml.

Protocols

  • Western blot protocols
  • Immunoprecipitation protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
  • References (1)

    Publishing research using ab156682? Please let us know so that we can cite the reference in this datasheet.

    ab156682 has been referenced in 1 publication.

    • Cera I  et al. Genes encoding SATB2-interacting proteins in adult cerebral cortex contribute to human cognitive ability. PLoS Genet 15:e1007890 (2019). PubMed: 30726206

    Customer reviews and Q&As

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