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    hnrnp-l-antibody-ab32680.pdf

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Tags & Cell Markers Subcellular Markers Nucleus Nucleoplasm
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Anti-hnRNP L antibody (ab32680)

  • Datasheet
  • SDS
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Immunocytochemistry/ Immunofluorescence - Anti-hnRNP L antibody (ab32680)
  • Western blot - Anti-hnRNP L antibody (ab32680)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP L antibody (ab32680)

Key features and details

  • Rabbit polyclonal to hnRNP L
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

You may also be interested in

Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-hnRNP L antibody
    See all hnRNP L primary antibodies
  • Description

    Rabbit polyclonal to hnRNP L
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 250 - 350 of Human hnRNP L.

    Read Abcam's proprietary immunogen policy (Peptide available as ab45315.)
  • Positive control

    • This antibody gave a positive signal in the following whole cell lysates: HeLa (Human epithelial carcinoma cell line); Jurkat (Human T cell lymphoblast-like cell line); A431 (Human epithelial carcinoma cell line); HEK293 (Human embryonic kidney cell line); HepG2 (Human hepatocellular liver carcinoma cell line); MCF7 (Human breast adenocarcinoma cell line); SHSY-5Y (Human neuroblastoma cell line) IHC-P: human normal cervix FFPE tissue sections
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Subcellular Markers
    • Nucleus
    • Nucleoplasm
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • RNA Processing
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • RNA Processing
    • Other

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

Applications

Our Abpromise guarantee covers the use of ab32680 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 60 kDa).
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 µg/ml.

Target

  • Function

    This protein is a component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. Is associated with most nascent transcripts including those of the landmark giant loops of amphibian lampbrush chromosomes. Associates, together with APEX1, to the negative calcium responsive element (nCaRE) B2 of the APEX2 promoter.
  • Sequence similarities

    Contains 3 RRM (RNA recognition motif) domains.
  • Post-translational
    modifications

    Several isoelectric forms of the L protein are probably the results of post-translational modifications.
  • Cellular localization

    Nucleus > nucleoplasm. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
  • Target information above from: UniProt accession P14866 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 3191 Human
    • Entrez Gene: 15388 Mouse
    • Omim: 603083 Human
    • SwissProt: P14866 Human
    • SwissProt: Q8R081 Mouse
    • Unigene: 446623 Human
    • Unigene: 644906 Human
    • Unigene: 9043 Mouse
    • Alternative names

      • D830027H13Rik antibody
      • FLJ35509 antibody
      • Heterogeneous nuclear ribonucleoprotein L antibody
      • hnRNP L antibody
      • hnRNP-L antibody
      • Hnrnpl antibody
      • hnRPL antibody
      • HNRPL_HUMAN antibody
      • P/OKcl.14 antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-hnRNP L antibody (ab32680)
      Immunocytochemistry/ Immunofluorescence - Anti-hnRNP L antibody (ab32680)
      ICC/IF image of ab32680 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in PBS-T (20 min) and incubated with the antibody (ab32680, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

    • Western blot - Anti-hnRNP L antibody (ab32680)
      Western blot - Anti-hnRNP L antibody (ab32680)
      All lanes : Anti-hnRNP L antibody (ab32680) at 1 µg/ml

      Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
      Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
      Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
      Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
      Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
      Lane 6 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
      Lane 7 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Performed under reducing conditions.

      Predicted band size: 60 kDa
      Observed band size: 65 kDa
      why is the actual band size different from the predicted?

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP L antibody (ab32680)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP L antibody (ab32680)
      IHC image of hnRNP L staining in human normal cervix formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab32680, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    Protocols

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (2)

    Publishing research using ab32680? Please let us know so that we can cite the reference in this datasheet.

    ab32680 has been referenced in 2 publications.

    • Mittenberg AG  et al. Combined treatment of human multiple myeloma cells with bortezomib and doxorubicin alters the interactome of 20S proteasomes. Cell Cycle 17:1745-1756 (2018). PubMed: 30009671
    • Gaudreau MC  et al. Heterogeneous Nuclear Ribonucleoprotein L is required for the survival and functional integrity of murine hematopoietic stem cells. Sci Rep 6:27379 (2016). ChIP . PubMed: 27271479

    Customer reviews and Q&As

    Show All Reviews Q&A
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    There are currently no Customer reviews or Questions for ab32680.
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