Anti-hnRNP L antibody (ab32680)
- Datasheet
- Specific References (1)
- Protocols
Overview
-
Product nameAnti-hnRNP L antibody
See all hnRNP L primary antibodies -
DescriptionRabbit polyclonal to hnRNP L
-
Host speciesRabbit
-
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
-
Species reactivityReacts with: Human
Predicted to work with: Mouse
-
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 250 - 350 of Human hnRNP L.
(Peptide available as ab45315.)
-
Positive control
- This antibody gave a positive signal in the following whole cell lysates: HeLa (Human epithelial carcinoma cell line); Jurkat (Human T cell lymphoblast-like cell line); A431 (Human epithelial carcinoma cell line); HEK293 (Human embryonic kidney cell line); HepG2 (Human hepatocellular liver carcinoma cell line); MCF7 (Human breast adenocarcinoma cell line); SHSY-5Y (Human neuroblastoma cell line) IHC-P: human normal cervix FFPE tissue sections
Properties
-
FormLiquid
-
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
-
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... -
PurityImmunogen affinity purified
-
ClonalityPolyclonal
-
IsotypeIgG
-
Research areas
Associated products
-
Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab32680 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 60 kDa). | |
| IHC-P | Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| ICC/IF | Use a concentration of 1 µg/ml. |
Target
-
FunctionThis protein is a component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. Is associated with most nascent transcripts including those of the landmark giant loops of amphibian lampbrush chromosomes. Associates, together with APEX1, to the negative calcium responsive element (nCaRE) B2 of the APEX2 promoter.
-
Sequence similaritiesContains 3 RRM (RNA recognition motif) domains.
-
Post-translational
modificationsSeveral isoelectric forms of the L protein are probably the results of post-translational modifications. -
Cellular localizationNucleus > nucleoplasm. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
- Information by UniProt
-
Database links
- Entrez Gene: 3191 Human
- Entrez Gene: 15388 Mouse
- Omim: 603083 Human
- SwissProt: P14866 Human
- SwissProt: Q8R081 Mouse
- Unigene: 446623 Human
- Unigene: 644906 Human
- Unigene: 9043 Mouse
-
Alternative names
- D830027H13Rik antibody
- FLJ35509 antibody
- Heterogeneous nuclear ribonucleoprotein L antibody
see all
Images
-
Western blot - Anti-hnRNP L antibody (ab32680)All lanes : Anti-hnRNP L antibody (ab32680) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 6 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 7 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 60 kDa
Observed band size: 65 kDa (why is the actual band size different from the predicted?) -
Immunocytochemistry/ Immunofluorescence - Anti-hnRNP L antibody (ab32680)ICC/IF image of ab32680 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in PBS-T (20 min) and incubated with the antibody (ab32680, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP L antibody (ab32680)IHC image of hnRNP L staining in human normal cervix formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab32680, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Protocols
References
This product has been referenced in:
- Gaudreau MC et al. Heterogeneous Nuclear Ribonucleoprotein L is required for the survival and functional integrity of murine hematopoietic stem cells. Sci Rep 6:27379 (2016). ChIP . Read more (PubMed: 27271479) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"