Recombinant

Recombinant Anti-hnRNP M1-M4 antibody [HL374] (ab255958)

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Immunocytochemistry/ Immunofluorescence - Anti-hnRNP M1-M4 antibody [HL374] (ab255958)
  • Western blot - Anti-hnRNP M1-M4 antibody [HL374] (ab255958)
  • Anti-hnRNP M1-M4 antibody [HL374] (ab255958)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [HL374] to hnRNP M1-M4
  • Suitable for: ICC/IF, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-hnRNP M1-M4 antibody [HL374]
    See all hnRNP M1-M4 primary antibodies

  • Description

    Mouse monoclonal [HL374] to hnRNP M1-M4

  • Host species

    Mouse

  • Tested applications

    Suitable for: ICC/IF, WBmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is considered to be commercially sensitive.

  • Positive control

    • WB: HeLa and MCF7 whole cell lysate. ICC/IF: HeLa cells.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab255958 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 9.26 µg/ml.
WB Use a concentration of 0.463 µg/ml. Detects a band of approximately 78 kDa (predicted molecular weight: 78 kDa).
  • Application notes
    Is unsuitable for IP.

    • Target

    • Function

      Pre-mRNA binding protein in vivo, binds avidly to poly(G) and poly(U) RNA homopolymers in vitro. Involved in splicing. Acts as a receptor for carcinoembryonic antigen in Kupffer cells, may initiate a series of signaling events leading to tyrosine phosphorylation of proteins and induction of IL-1 alpha, IL-6, IL-10 and tumor necrosis factor alpha cytokines.

    • Sequence similarities

      Contains 3 RRM (RNA recognition motif) domains.

    • Post-translational
      modifications

      Sumoylated.

    • Cellular localization

      Nucleus > nucleolus.
    • Information by UniProt

    • Database links

      • Alternative names

        • CEA receptor antibody
        • CEAR antibody
        • DKFZp547H118 antibody
        • Heterogeneous nuclear ribonucleoprotein M antibody
        • Heterogeneous nuclear ribonucleoprotein M4 antibody
        • Heterogenous nuclear ribonucleoprotein M4 antibody
        • hnRNA binding protein M4 antibody
        • hnRNP M antibody
        • Hnrnpm antibody
        • HNRNPM4 antibody
        • HNRPM antibody
        • HNRPM_HUMAN antibody
        • HNRPM4 antibody
        • HTGR1 antibody
        • N acetylglucosamine receptor 1 antibody
        • NAGR1 antibody
        see all

      Images

      • Immunocytochemistry/ Immunofluorescence - Anti-hnRNP M1-M4 antibody [HL374] (ab255958)
        Immunocytochemistry/ Immunofluorescence - Anti-hnRNP M1-M4 antibody [HL374] (ab255958)

        Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labeling hnRNP M1-M4 with ab255958 at 9.26µg/ml, followed by Goat Anti-Rat IgG (Alexa Fluor® 488) (ab150157) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear staining in HeLa cell line. The nuclear counterstain is DAPI (blue).
        Tubulin is detected with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1/200 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 594) (ab150080) secondary antibody at 1/1000 dilution (red).

      • Western blot - Anti-hnRNP M1-M4 antibody [HL374] (ab255958)
        Western blot - Anti-hnRNP M1-M4 antibody [HL374] (ab255958)
        All lanes : Anti-hnRNP M1-M4 antibody [HL374] (ab255958) at 0.463 µg/ml

        Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
        Lane 2 : MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate

        Lysates/proteins at 20 µg per lane.

        Secondary
        All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/5000 dilution

        Predicted band size: 78 kDa



        Blocking/Dilution buffer: 5% NFDM/TBST.

        Exposure time: Lane 1: 1 sec; Lane 2: 3 secs.

      • Anti-hnRNP M1-M4 antibody [HL374] (ab255958)
        Anti-hnRNP M1-M4 antibody [HL374] (ab255958)

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      References (1)

      Publishing research using ab255958? Please let us know so that we can cite the reference in this datasheet.

      ab255958 has been referenced in 1 publication.

      • Datar KV  et al. The human hnRNP M proteins: identification of a methionine/arginine-rich repeat motif in ribonucleoproteins. Nucleic Acids Res 21:439-46 (1993). PubMed: 8441656

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