Key features and details
- Mouse monoclonal [2A6-2H3] to hnRNP M3-M4
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG2b
Product nameAnti-hnRNP M3-M4 antibody [2A6-2H3]
See all hnRNP M3-M4 primary antibodies
DescriptionMouse monoclonal [2A6-2H3] to hnRNP M3-M4
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Human
Full length native Human proteins hnRNP M3-M4 (NP_005959.2, NP_112480.2)
- 293T cell lysate, HeLa cell lysate, Jurkat cell lysate, NIH 3T3 cell lysate
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at -20°C. The product can be stored for up to 12 months.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 99% Tris buffered saline, 0.1% BSA
pH 7 to 8
Concentration information loading...
Purification notesab128143 was produced via Mouse Hybridoma. The hybridoma was grown in a bioreactor and the antibody was purified using a mouse immunoglobulin binding ligand.
Our Abpromise guarantee covers the use of ab128143 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 77 kDa.|
|IP||Use at 2-5 µg/mg of lysate.|
FunctionPre-mRNA binding protein in vivo, binds avidly to poly(G) and poly(U) RNA homopolymers in vitro. Involved in splicing. Acts as a receptor for carcinoembryonic antigen in Kupffer cells, may initiate a series of signaling events leading to tyrosine phosphorylation of proteins and induction of IL-1 alpha, IL-6, IL-10 and tumor necrosis factor alpha cytokines.
Sequence similaritiesContains 3 RRM (RNA recognition motif) domains.
Cellular localizationNucleus, nucleolus.
- Information by UniProt
- CEA receptor antibody
- CEAR antibody
- Heterogeneous nuclear ribonucleoprotein M antibody
All lanes : Anti-hnRNP M3-M4 antibody [2A6-2H3] (ab128143) at 0.04 µg/ml
Lane 1 : 293T cell lysate at 50 µg
Lane 2 : 293T cell lysate at 15 µg
Lane 3 : HeLa cell lysate at 50 µg
Lane 4 : Jurkat cell lysate at 50 µg
Lane 5 : NIH 3T3 cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 77 kDa
Exposure time: 30 seconds
Immunoprecipitation : 1 mg of 293T cell lysate immunoprecipitated with ab128143 at 3 µg/mg lysate; 20% of immunoprecipitate loaded in lane
Western Blot: ab128143 at 0.04 µg/ml
developed using the ECL technique
Exposure time : 30 seconds
Predicted band size : 77 kDa
ab128143 has not yet been referenced specifically in any publications.