Overview

  • Product name

    Anti-hnRNP M3-M4 antibody [2A6-2H3]
    See all hnRNP M3-M4 primary antibodies
  • Description

    Mouse monoclonal [2A6-2H3] to hnRNP M3-M4
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Full length native Human proteins hnRNP M3-M4 (NP_005959.2, NP_112480.2)

  • Positive control

    • 293T cell lysate, HeLa cell lysate, Jurkat cell lysate, NIH 3T3 cell lysate
  • General notes

    Shelf Life: 1 year from date of receipt

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 99% Tris buffered saline, 0.1% BSA

    pH 7 to 8
  • Concentration information loading...
  • Purity

    IgG fraction
  • Purification notes

    ab128143 was produced via Mouse Hybridoma. The hybridoma was grown in a bioreactor and the antibody was purified using a mouse immunoglobulin binding ligand.
  • Clonality

    Monoclonal
  • Clone number

    2A6-2H3
  • Isotype

    IgG2b
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab128143 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 77 kDa.
IP Use at 2-5 µg/mg of lysate.

Target

  • Function

    Pre-mRNA binding protein in vivo, binds avidly to poly(G) and poly(U) RNA homopolymers in vitro. Involved in splicing. Acts as a receptor for carcinoembryonic antigen in Kupffer cells, may initiate a series of signaling events leading to tyrosine phosphorylation of proteins and induction of IL-1 alpha, IL-6, IL-10 and tumor necrosis factor alpha cytokines.
  • Sequence similarities

    Contains 3 RRM (RNA recognition motif) domains.
  • Post-translational
    modifications

    Sumoylated.
  • Cellular localization

    Nucleus, nucleolus.
  • Information by UniProt
  • Database links

  • Alternative names

    • CEA receptor antibody
    • CEAR antibody
    • Heterogeneous nuclear ribonucleoprotein M antibody
    • Heterogenous nuclear ribonucleoprotein M4 antibody
    • hnRNA binding protein M4 antibody
    • hnRNP M antibody
    • Hnrnpm antibody
    • HNRNPM4 antibody
    • HNRPM antibody
    • HNRPM_HUMAN antibody
    • HNRPM4 antibody
    • HTGR1 antibody
    • M3 antibody
    • M4 antibody
    • M4 protein antibody
    • N-acetylglucosamine receptor 1 antibody
    • NAGR1 antibody
    see all

Images

  • All lanes : Anti-hnRNP M3-M4 antibody [2A6-2H3] (ab128143) at 0.04 µg/ml

    Lane 1 : 293T cell lysate at 50 µg
    Lane 2 : 293T cell lysate at 15 µg
    Lane 3 : HeLa cell lysate at 50 µg
    Lane 4 : Jurkat cell lysate at 50 µg
    Lane 5 : NIH 3T3 cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 77 kDa


    Exposure time: 30 seconds
  • Immunoprecipitation : 1 mg of 293T cell lysate immunoprecipitated with ab128143 at 3 µg/mg lysate; 20% of immunoprecipitate loaded in lane

    Western Blot: ab128143 at 0.04 µg/ml

    developed using the ECL technique

    Exposure time : 30 seconds

    Predicted band size : 77 kDa

References

ab128143 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab128143.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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