Product nameAnti-hnRNP M3-M4 antibody
See all hnRNP M3-M4 primary antibodies
DescriptionRabbit polyclonal to hnRNP M3-M4
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Elephant
- 293T, HeLa, Jurkat and NIH 3T3 whole cell lysates.
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab157100 was affinity purified using an epitope specific to hnRNP M3-M4 immobilized on solid support.
Our Abpromise guarantee covers the use of ab157100 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 77 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionPre-mRNA binding protein in vivo, binds avidly to poly(G) and poly(U) RNA homopolymers in vitro. Involved in splicing. Acts as a receptor for carcinoembryonic antigen in Kupffer cells, may initiate a series of signaling events leading to tyrosine phosphorylation of proteins and induction of IL-1 alpha, IL-6, IL-10 and tumor necrosis factor alpha cytokines.
Sequence similaritiesContains 3 RRM (RNA recognition motif) domains.
Cellular localizationNucleus, nucleolus.
- Information by UniProt
- CEA receptor antibody
- CEAR antibody
- Heterogeneous nuclear ribonucleoprotein M antibody
All lanes : Anti-hnRNP M3-M4 antibody (ab157100) at 0.1 µg/ml
Lane 1 : 293T whole cell lysate at 50 µg
Lane 2 : 293T whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Lane 5 : NIH 3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 77 kDa
Exposure time: 3 minutes
Immunoprecipitation/ Western Blot of hnRNP M3-M4. Detection of hnRNP M3-M4 in Immunoprecipitates of 293T whole cell lysates (1 mg for IP, 20% of IP loaded) using ab157100 at 6 µg/mg lysate for IP (Lane 1). For WB detection an ab157100 was used at 0.4 µg/ml. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 10 seconds.
All lanes : Anti-hnRNP M3-M4 antibody (ab157100)
Lane 1 : whole cell lysate at 6 µg/ml
Lane 2 : Control IgG
ab157100 has not yet been referenced specifically in any publications.