Anti-hnRNP Q antibody (ab189405)
Key features and details
- Rabbit polyclonal to hnRNP Q
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-hnRNP Q antibody
See all hnRNP Q primary antibodies -
Description
Rabbit polyclonal to hnRNP Q -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IF, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide within Human hnRNP Q aa 200-300. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. (NP_006363.4).
Database link: O60506 -
Positive control
- Human brain, neurons, tissue; HepG2 cells; HUVEC, Jurjkat and 293 cell extracts.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 0.87% Sodium chloride, 50% Glycerol (glycerin, glycerine)
PBS without Mg2+ and Ca2+. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab189405 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use a concentration of 20 µg/ml.
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ICC/IF |
1/100 - 1/500.
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WB |
1/500 - 1/1000. Predicted molecular weight: 70 kDa.
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Notes |
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IHC-P
Use a concentration of 20 µg/ml. |
ICC/IF
1/100 - 1/500. |
WB
1/500 - 1/1000. Predicted molecular weight: 70 kDa. |
Target
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Function
Heterogenous nuclear ribonucleoprotein (hnRNP) implicated in mRNA processing mechanisms. Component of the CRD-mediated complex that promotes MYC mRNA stability. Isoform 1, isoform 2 and isoform 3 are associated in vitro with pre-mRNA, splicing intermediates and mature mRNA protein complexes. Isoform 1 binds to apoB mRNA AU-rich sequences. Isoform 1 is part of the APOB mRNA editosome complex and may modulate the postranscriptional C to U RNA-editing of the APOB mRNA through either by binding to A1CF (APOBEC1 complementation factor), to APOBEC1 or to RNA itself. May be involved in translationally coupled mRNA turnover. Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain. Interacts in vitro preferentially with poly(A) and poly(U) RNA sequences. Isoform 3 may be involved in cytoplasmic vesicle-based mRNA transport through interaction with synaptotagmins. -
Tissue specificity
Ubiquitously expressed. Detected in heart, brain, pancreas, placenta, spleen, lung, liver, skeletal muscle, kidney, thymus, prostate, uterus, small intestine, colon, peripheral blood and testis. -
Sequence similarities
Contains 3 RRM (RNA recognition motif) domains. -
Domain
The domain containing eight Arg-Gly-Gly repeats may be involved in RNA-binding and protein-protein interactions. -
Post-translational
modificationsPhosphorylated on tyrosine. The membrane-bound form found in microsomes is phosphorylated in vitro by insulin receptor tyrosine kinase (INSR). Phosphorylation is inhibited upon binding to RNA, whereas the cytoplasmic form is poorly phosphorylated (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Nucleus > nucleoplasm. Expressed predominantly in the nucleoplasm and Cytoplasm. Microsome. Endoplasmic reticulum. Nucleus. The tyrosine phosphorylated form bound to RNA is found in microsomes (By similarity). Localized in cytoplasmic mRNP granules containing untranslated mRNAs. - Information by UniProt
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Database links
- Entrez Gene: 10492 Human
- Entrez Gene: 56403 Mouse
- Entrez Gene: 363113 Rat
- SwissProt: O60506 Human
- SwissProt: Q7TMK9 Mouse
- SwissProt: Q7TP47 Rat
- Unigene: 571177 Human
- Unigene: 260545 Mouse
see all -
Alternative names
- cytoplasmic RNA-interacting protein antibody
- dJ3J17.2 antibody
- Glycine and tyrosine rich RNA binding protein antibody
see all
Images
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All lanes : Anti-hnRNP Q antibody (ab189405) at 1/500 dilution
Lane 1 : HUVEC cell extract
Lane 2 : Jurkat cell extract
Lane 3 : 293 cell extract
Lane 4 : 293 cell extract with immunizing peptide
Predicted band size: 70 kDa -
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human brain, neurons, tissue labeling hnRNP Q with ab189405 at 20 µg/ml.
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Immunofluorescent analysis of HepG2 cells labeling hnRNP Q with ab189405 at 1/100 dilution, in the presence (right panel) or absence (left panel) of immunizing peptide.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab189405 has been referenced in 1 publication.
- Lai MC et al. Haplotype-specific MAPT exon 3 expression regulated by common intronic polymorphisms associated with Parkinsonian disorders. Mol Neurodegener 12:79 (2017). PubMed: 29084565