Anti-hnRNP R antibody (ab151251)
Key features and details
- Rabbit polyclonal to hnRNP R
- Suitable for: WB
- Knockout validated
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-hnRNP R antibody
See all hnRNP R primary antibodies -
Description
Rabbit polyclonal to hnRNP R -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Chicken, Cow -
Immunogen
Recombinant fragment corresponding to a region within amino acids 1 and 187 of Human hnRNP R (Uniprot ID: O43390)
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Positive control
- WB: Wild-type HEK-293T, MOLT-4, Caco-2, 293T, A431 and HeLa cell lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab151251 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 71 kDa.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 71 kDa. |
Target
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Function
Component of ribonucleosomes, which are complexes of at least 20 other different heterogenious nuclear ribonucleoproteins (hnRNP). hnRNP play an important role in processing of precursor mRNA in the nucleus. -
Sequence similarities
Contains 3 RRM (RNA recognition motif) domains. -
Cellular localization
Nucleus > nucleoplasm. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. - Information by UniProt
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Database links
- Entrez Gene: 419570 Chicken
- Entrez Gene: 512479 Cow
- Entrez Gene: 10236 Human
- Entrez Gene: 74326 Mouse
- Entrez Gene: 319110 Rat
- Omim: 607201 Human
- SwissProt: O43390 Human
- Unigene: 373763 Human
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Alternative names
- FLJ25714 antibody
- Heterogeneous nuclear ribonucleoprotein R antibody
- hnRNP R antibody
see all
Images
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All lanes : Anti-hnRNP R antibody (ab151251) at 1/500 dilution
Lane 1 : Wild-type HEK-293T cell lysate
Lane 2 : HNRNPR knockout HEK-293T cell lysate
Lane 3 : MOLT-4 cell lysate
Lane 4 : Caco-2 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 71 kDa
Observed band size: 78 kDa why is the actual band size different from the predicted?False colour image of Western blot: Anti-hnRNP R antibody staining at 1/500 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab151251 was shown to bind specifically to hnRNP R. A band was observed at 78 kDa in wild-type HEK-293T cell lysates with no signal observed at this size in HNRNPR knockout cell line ab266723 (knockout cell lysate ab257992). To generate this image, wild-type and HNRNPR knockout HEK-293T cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
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All lanes : Anti-hnRNP R antibody (ab151251) at 1/5000 dilution
Lane 1 : WT 293T cell extracts
Lane 2 : hnRNP R Knockout 293T cell extracts
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 71 kDa -
All lanes : Anti-hnRNP R antibody (ab151251) at 1/3000 dilution
Lane 1 : 293T cell whole lysate
Lane 2 : A431 cell whole lysate
Lane 3 : HeLa cell whole lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 71 kDa
7.5% SDS PAGE
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab151251 has not yet been referenced specifically in any publications.